Nicolas Servant

This paper introduces an alignment-last approach to transcriptomic analysis, enabling rapid biomarker discovery by using reads directly and revealing new transposable-element signatures and prognostic reads in cancer.

The paper investigates the role of CTCF and cohesin in X-inactivation escapee domains and finds that these domains are self-sustaining chromatin compartments independent of CTCF and cohesin.

The paper shows clonal memory in human HSPCs persists across divisions and is disrupted in AML, with potential for therapeutic modulation.

The paper shows that Wnt signaling in mesenchymal TNBC cells increases inflammation and EMT-related gene expression, and identifies a 55-gene signature associated with these effects.

The paper introduces OneCell CUT&Tag, a single-cell multi-omics method that captures chromatin, transcriptome, and surface marker data, revealing epigenomic reprogramming during mammary gland basal-to-luminal transition.

This study shows that ether lipids in cancer cells regulate membrane properties to influence iron uptake, ferroptosis, and metastasis, offering a therapeutic target.

The paper investigates a BRCA2 truncating variant's effect on NF-κB-driven transcription by showing it disrupts the BRCA2-PCAF complex, reducing histone acetylation and impairing cell migration.

This study identifies ORF1p interactions with proteins and chromatin in neurons, showing its role in gene regulation and neurite morphology.

The paper identifies an endogenous retroviral element that uses an upstream enhancer for somatic expression and mobility in Drosophila intestine.

The paper maps X-linked gene reactivation and epigenetic silencing in developing PGCs, showing partial resistance to reactivation and enrichment of repressive marks.

This study shows CCG interruptions in DM1 are unstable and hypermethylated, contributing to CTG repeat stabilization.

The paper shows that conserved RNA-binding protein interactions mediate the function of syntologous lncRNAs despite lacking sequence similarity.

This study shows Xist RNA modulates X-chromosome inactivation beyond early development, reversibly silencing escapee genes and altering topological domain structures in mouse embryos.

The paper investigates LINE-1 ORF1p protein and RNA expression in mouse and human brains, finding increased levels with aging and neuron-specific expression patterns.

The paper identifies microproteins encoded by intronic polyadenylation isoforms in the 5' part of genes and shows their role in modulating cisplatin response in cancer cells.

The paper uses multi-omics analysis to compare malignant and normal uveal melanocytes, identifying genomic instability and a BAP1 promoter deletion as key features of uveal melanoma.

The paper shows that intronic polyadenylation (IPA) of MET affects tumour progression and therapy resistance by producing a short MET isoform that reduces invasiveness and enhances response to MAPK-targeted therapy.

The paper shows that post-transcriptional PAS cleavage of p53 pre-mRNA allows it to escape DNA damage inhibition, and identifies other DDR-related pre-mRNAs with similar mechanisms.

This study identifies mitotic recombination as the primary mechanism for loss of heterozygosity in Drosophila intestinal stem cells, showing increased LOH frequency with specific bacterial infection.

The paper inverts a topological domain and shows it causes limited gene expression changes but affects inter-domain communication, particularly in Xist expression.

ChromSCape is an R/Shiny tool for analyzing single-cell chromatin data, enabling subpopulation identification and differential region analysis in epigenomic studies.

This study identifies EZHIP as a novel cofactor of PRC2 that limits its activity in germ cells, leading to altered H3K27me2/3 deposition and impaired fertility in female mice.

This paper introduces a normalization method for Hi-C data that accounts for copy number variations, improving downstream analysis of genome organization.

The paper identifies BAP1 as a general transcriptional co-activator through CRISPR/Cas9-generated isogenic cell lines and shows it functions independently of the Polycomb machinery.

The paper investigates X-linked gene reactivation in mouse inner cell mass by analyzing epigenetic marks and transcription factors, finding differential reactivation speeds and mechanisms.