Jonathan B. Grimm

A unified photosensitizer platform for in situ DNA, RNA, and protein directed proximity labeling

The paper introduces POCA, a photosensitizer platform for proximity labeling of proteins, RNA, and DNA in fixed cells without genetic engineering, demonstrating its utility across multiple molecular targets.

Relative phase of membrane potential theta oscillations between individual hippocampal neurons code space

The paper analyzes the relative phase of membrane potential theta oscillations between hippocampal neurons during a virtual spatial task, revealing spatially coded phase shifts independent of spiking activity.

Design of Orthogonal Far-Red, Orange and Green Fluorophore-binding Proteins for Multiplex Imaging

The paper designs orthogonal fluorophore-binding proteins using deep learning for multiplex imaging, demonstrating high specificity and utility in labeling and monitoring subcellular processes.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy

This paper designs de novo Rhodamine Binder (Rhobin) tags with high brightness, stability, and applicability in live-cell imaging and extreme conditions.

In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors

The paper introduces a far-red dopamine sensor, HaloDA1.0, enabling multiplex imaging of neurochemical networks in vivo with high sensitivity and versatility.

FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly

FilaBuster enables rapid, specific, and spatiotemporally controlled disassembly of intermediate filaments in living cells using a three-step photochemical strategy.

Imaging the extracellular matrix in live tissues and organisms with a glycan-binding fluorophore

This paper introduces a fluorophore for imaging extracellular matrix in live tissues and organisms by binding to glycans, enabling nonperturbative and wash-free visualization.

Dendritic excitations govern back-propagation via a spike-rate accelerometer

The paper investigates dendritic excitations in CA1 pyramidal neurons, showing how locally generated Na+ spikes drive history-dependent spike back-propagation and influence associative plasticity.

A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light

The paper introduces WHaloCaMP, a near-infrared calcium indicator, and demonstrates its use in imaging calcium responses in various organisms.

Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation

The paper introduces EPSILON, a technique to map AMPA receptor dynamics during memory formation and finds a correlation between synaptic plasticity and cFos expression.

Voltage dynamics of dendritic integration and back-propagation in vivo

The paper measures voltage dynamics in dendrites of mice neurons during synaptic input integration and action potential propagation, finding shared membrane voltage and rate-dependent propagation of back-propagating action potentials.

Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator

The paper identifies a mutation that enhances the sensitivity of a fluorescent voltage indicator, improving its ability to detect both spiking and subthreshold voltage signals in multiple organisms.

Time-tagged ticker tapes for intracellular recordings

The paper develops a protein-based ticker tape system to record neural activation histories with high temporal resolution.

Brain-wide measurement of synaptic protein turnover reveals localized plasticity during learning

The paper develops DELTA, a method to measure brain-wide synaptic protein turnover at single-synapse resolution, revealing localized plasticity in hippocampal CA1 during learning.

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

The paper introduces a new genetically encoded voltage indicator, Voltron, which improves brightness and photostability for extended in vivo voltage imaging in mice, zebrafish, and fruit flies.