David Clifford Henshall

CDKL5 deficiency alters cell subtype-specific molecular programmes and intercellular signalling in the mouse hippocampus

This study uses snRNA-seq to analyze gene expression changes in the hippocampus of CDKL5-deficient mice, identifying cell-type-specific transcriptional alterations and intercellular signaling changes.

VECTR-Clasp: An open machine-learning and vector-based framework for objective quantification of motor dysfunction during hind-limb clasping in Cdkl5-deficient mice

VECTR-Clasp provides a continuous geometric analysis of motor behavior in Cdkl5-deficient mice, revealing previously undetected motor microphenotypes.

Spatiotemporal transcriptomics reveals distinct responses of ALDH1A1-positive and ALDH1A1-negative midbrain dopaminergic neurons to alpha-synuclein overexpression

This study uses spatial transcriptomics to show that ALDH1A1+ and ALDH1A1- midbrain dopaminergic neurons respond differently to alpha-synuclein overexpression, with distinct transcriptional changes over time.

Attenuated single neuron and network hyperexcitability following microRNA-134 inhibition in mice with drug-resistant temporal lobe epilepsy

This study shows inhibiting miR-134 reduces single neuron and network hyperexcitability in mice with drug-resistant temporal lobe epilepsy.

Shared and distinct microRNA profiles between HT22, N2A and SH-SY5Y cell lines and primary mouse hippocampal neurons

The paper compares miRNA profiles across HT22, N2A, SH-SY5Y cell lines and primary mouse hippocampal neurons to assess their similarity to primary neurons.

Transcriptomic profiles from stereo-EEGs reveal the local cell microenvironment in human epilepsy

The paper uses transcriptomic data from SEEG electrodes to characterize the molecular microenvironment in human epilepsy, showing gene expression reflects local brain activity and cell types.

Endothelial tight junctions and cell-matrix adhesions reciprocally control blood-brain barrier integrity

This study shows that tight junctions and cell-matrix adhesions in brain endothelial cells reciprocally control blood-brain barrier integrity through mechanical forces and protein expression changes.

A stolen future: aberrant hippocampal neurogenesis produces glial cells in epilepsy

The paper investigates how epilepsy causes early neuroblasts to switch fate to astrocytes in both mouse and human tissue using immunolabelling and single-cell RNA sequencing.

High-Resolution Multimodal Profiling of Human Epileptic Brain Activity via Explanted Depth Electrodes

This study uses explanted SEEG electrodes to integrate RNA sequencing, DNA methylation, and genomic data with electrophysiological recordings, revealing distinct molecular landscapes in epilepsies and identifying potential seizure zones.

EPILEPTOGENESIS INHIBITS THE CIRCADIAN CLOCK AND RESHAPES THE DIURNAL TRANSCRIPTOMIC RHYTHMICITY IN THE MOUSE HIPPOCAMPUS

This study shows that epileptogenesis disrupts the circadian clock and alters diurnal transcriptomic rhythms in the mouse hippocampus, with implications for human epilepsy.

Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsy

The paper identifies brain-originating microRNAs in the blood of mice with experimental epilepsy, supporting their use as biomarkers.

MicroRNA-335-5p suppresses voltage-gated sodium channel expression and may be a target for seizure control

This study identifies miR-335-5p as a regulator of voltage-gated sodium channels, showing that its modulation can influence seizure activity in epilepsy models.

Immunoproteasome deficiency results in accelerated brain aging and epilepsy

This study shows that immunoproteasome deficiency accelerates brain aging and causes epilepsy in mice, with reduced immunoproteasome expression also observed in human epilepsy patients.

A role for myeloid miR-155 in regulating hypoxia induced seizures in neonatal C57BL/J6 mice

This study shows that deleting miR-155 in myeloid cells reduces seizure severity and inflammation in a neonatal hypoxia-induced seizures model.

MicroRNA inhibition using antimiRs in acute human brain tissue sections

This paper demonstrates the effectiveness of antimiR-134 in reducing miR-134 levels in human brain tissue sections, supporting its potential as a treatment for pharmacoresistant epilepsy.

Sexual dimorphism in epilepsy and comorbidities in Dravet syndrome mice carrying a targeted deletion of exon 1 of the Scn1a gene

This study investigates sex differences and long-term comorbidities in Scn1a(+/-)tm1Kea mice modeling Dravet Syndrome, finding sex-specific seizure patterns and reduced behavioral impairments in older mice.

Functional expression of the ATP-gated P2X7 receptor in human iPSC-derived neurons and astrocytes

This study demonstrates functional P2X7R expression in human iPSC-derived neurons and astrocytes using calcium imaging and immunostaining.

High concordance between hippocampal transcriptome of the intraamygdala kainic acid model and human temporal lobe epilepsy

The paper compares gene expression in a mouse model of status epilepticus to human temporal lobe epilepsy tissue, finding high concordance in gene dysregulation and shared molecular features.

Potent and lasting seizure suppression by systemic delivery of antagomirs targeting miR-134 timed with blood-brain barrier disruption

This study shows that systemic delivery of Ant-134, timed with BBB disruption after status epilepticus, suppresses seizures in mice with long-lasting effects.

A systems approach delivers a functional microRNA catalog and expanded targets for seizure suppression in temporal lobe epilepsy

The paper identifies functional miRNAs and their targets involved in seizure suppression in temporal lobe epilepsy using animal models and functional screens.

Large-scale changes to mRNA polyadenylation in temporal lobe epilepsy

This study identifies widespread changes in mRNA polyadenylation in temporal lobe epilepsy, highlighting the role of CPEB4 in seizure severity and neurodegeneration.