CRISPR screen

The paper identifies a regulatory SNP rs60464856 that increases RUVBL1 expression and contributes to prostate cancer progression through cohesin-CTCF binding and cell-cycle activation.

This study shows that cooperative self-assembly of transcriptional regulators can improve regulatory specificity and long-term stability of synthetic gene circuits in yeast.

The paper uses CRISPR/Cas9 screens to identify essential MYC binding sites and target genes in cancer cells, revealing key genes involved in cancer pathways.

This study uses hiPSC-derived cardiomyocytes to assess MYH7 variants' effects, showing pathogenic variants reduce protein abundance and cell survival.

This study uses a CRISPR screen to identify HIV-1 latency reversal factors, finding that CCNT1 is non-essential for T cell activation but critical for HIV reactivation from latency.

The paper uses a CRISPRi screen in hESC differentiation to identify enhancers with nonlinear regulatory activity, revealing a CTCF loop-constrained model for improved enhancer prediction.

The paper introduces CCTK, a toolkit for analyzing CRISPR array diversity, including tools for identifying arrays, visualizing similarities, and inferring phylogenetic trees.

This paper introduces SMuRF, a framework for deep mutational scanning in disease-related genes, generating functional scores for all coding variants and aiding in variant interpretation.

This study compares robotic and manual injection methods for CRISPR-CasRx RNA knockdown in zebrafish embryos, finding similar efficiency and phenotype rates between approaches.

The paper reports the structure of AsCas12f1 and engineers it to enhance genome editing activity in human cells.

The paper identifies NFATC2IP as a mediator of SUMO-dependent genome integrity through CRISPR/Cas9 screens and functional validation.

This study identifies epigenomic and sequence features that influence prime editing efficiency and provides guidelines for optimizing prime editor designs.

This study shows that post-transplant G-CSF administration impairs engraftment of CRISPR-edited HSPCs by worsening the p53 DNA damage response, but not unedited or lentiviral-transduced HSPCs.

This paper describes a genome editing strategy to reprogram human B cells to express custom heavy chain antibodies that respond to immunization.

This study shows that HD mutations cause a dominant negative effect on HTT function by inducing HD-like phenotypes in non-HD cells.

This study disrupts thymidylate synthase to control cell proliferation in cell therapies without affecting cell function.

This paper describes a method to engineer large focal amplifications in primary cells using ecDNAs, showing they promote proliferation and transformation.

This paper develops FUS-CRISPR(a/i) tools to remotely control gene expression and epigenetic modifications in vivo, showing therapeutic effects in tumor models.

The paper introduces a CRISPR/Cas9 HDR plasmid platform for Drosophila with higher efficiency than existing methods, enabling new genetic experiments.

The paper introduces CrAAVe-seq, an AAV-based CRISPR screening platform for in vivo cell type-specific analysis, demonstrating its scalability and sensitivity in identifying neuron-essential genes.

This study identifies conditionally essential genes in Zymomonas mobilis for aerobic and anaerobic growth using CRISPRi-seq, revealing roles of DNA repair and respiratory complexes in energy coupling and isoprenoid biosynthesis.

The paper develops a modular exosome-based platform for targeted CRISPR/Cas9 genome editing, demonstrating efficient delivery and gene editing in vitro and in vivo.

The paper systematically evaluates the transferability of synthetic biology parts across multiple bacterial hosts and identifies host-specific variations in gene expression.

acCRISPR improves CRISPR screen accuracy by correcting sgRNA activity, identifying essential genes in yeast under various conditions.

The paper shows that NbaAgo forms a complex with an effector nuclease to cleave DNA/RNA upon target recognition, providing bacterial immunity against invaders.

This paper develops an allele-specific gene editing approach to target the T17M RHO mutant allele in retinitis pigmentosa, showing improved retinal function and photoreceptor preservation in mice.

CasTuner enables analog tuning of endogenous gene expression using CRISPR/Cas systems and a degron domain, showing dose-dependent repression and application in studying gene dosage effects.

The paper identifies compact CRISPR-based activators with durable gene activation, improving upon existing tools in potency and persistence.

This study uses CRISPR-Cas9 to edit the endogenous {delta}-globin promoter to increase HbA2 expression in human erythroid cells, showing that multiple transcription factor elements are required for effective upregulation.