CRISPR screen

This paper describes a workflow for dual-guide CRISPR-Cas9 editing of Pla2g6 in mouse BMDMs, showing high editing efficiency and specific deletion patterns.

This study uses CRISPRi-seq to identify essential genes affecting aminoglycoside susceptibility in Pseudomonas aeruginosa, revealing gene-specific fitness effects and potential targets for antibiotic enhancement.

The paper uses base editing screens to identify second-site mutations that correct misfolded NPC1 proteins, enabling therapeutic applications for diseases like Niemann-Pick type C.

This study edits mtDNA variants in human iPSCs to show that heteroplasmic complex I variants impair neuronal commitment.

This paper develops CMD-coated magnetic nanoparticles for delivering CRISPR RNPs, demonstrating efficient genome editing in cell lines and a skin disorder model.

PrEditR is a tool for designing sgRNAs for CRISPR-based base editors, allowing users to target specific amino acids and annotate resulting mutations.

This paper develops a tumor-targeting engineered bacterium that improves therapeutic efficacy and safety in cancer treatment.

This study identifies genetic vulnerabilities from chromosome loss events in cancer by analyzing CRISPR screening data and copy number profiles, revealing paralogous gene dependencies as therapeutic targets.

A genome-integrated Cas12m in E. coli shows no measurable growth cost, reverses repression faster than dCas9, and supports NOT gates, a two-layer buffer and a two-pathway pigment circuit.

This study evaluates Cas9 fusion proteins in Drosophila to enhance homing gene drive performance by improving HDR and reducing resistance.

The paper describes the development of En-ISPmu1, a modified TnpB nuclease, and demonstrates its ability to achieve targeted genome integration in mouse liver with a single AAV vector.

This study shows that Parkin deficiency alters genetic dependencies for neuronal survival, identifying key pathways and genes involved in this rewiring.

This study identifies SLC25A32 as a critical mitochondrial folate transporter that becomes essential in serine/glycine-starved triple-negative breast cancer, making it a potential therapeutic target.

The paper identifies and characterizes U6 promoters in Ulva prolifera, showing their dual transcriptional activity and utility for CRISPR-Cas9 genome editing.

CRISPR-dPCR quantifies CRISPR knock-in by distinguishing integration from donor abundance and background, using multiplex digital PCR with orthogonal strategies.

The paper uses CRISPR-LICHT to screen SOX2 targets in human brain organoids, identifying 28 new genes involved in neural progenitor lineage expansion and survival.

This study develops a light-responsive lipid nanoparticle platform for VEGFA gene editing to suppress choroidal neovascularisation, showing reduced vascular leakage and structural abnormalities in a rat model.

This study uses multiple functional genomic approaches to identify and characterize cis-regulatory elements (CREs) influencing LDLR expression, revealing overlapping yet distinct CREs across methods.

This study identifies 38 expression-modulating variants associated with neurodevelopmental disorders and links them to neurodevelopmental transcriptional programs using MPRA and CROP-seq.

Quality-controlled, cycle-certified E-Flux Human-GEM models of twelve regions from one prostate tumor curb implementation-driven flux inflation and show predicted metabolic similarity tracks spatial region, not histology.

The paper develops a CRISPR guide-discovery pipeline using locally deployed AI agents and evaluates it with large language models.

Host-measured parts and inducible systems for Brucella melitensis ΔvjbR guided a modeled Cas9 kill-switch, which cut mouse liver bacterial burden by about 91% at 30 mg/kg doxycycline.

This study designs synthetic activators for programmable gene expression by engineering coactivator-binding proteins and demonstrates their enhanced transcriptional activation capabilities.

This paper demonstrates efficient gene editing and phenotype generation in avian embryos using lipid nanoparticles as a delivery platform, showing successful CRISPR/Cas9 editing and long-term labeling of cranial NCC derivatives.

The paper introduces OPTIMuS PRIME-Del, a CRISPR-based method for multiplexed, precise deletion of cis-regulatory elements, validated through molecular tagging and scRNA-seq analysis.

The paper generates isogenic iPSC lines with CAG repeats and HTT KO for Huntington's disease study, showing differentiation potential and altered gene expression in neurons and astrocytes.

The paper identifies a compact regulatory logic governing fibroblast plasticity in fibrosis by analyzing enhancer repression and gene perturbations across multiple tissues.

This paper introduces ARI-HDR, a method for efficient internal-exon editing using splicing-guaranteed artificial introns, achieving over 80% editing efficiency in human genes.

This paper characterizes DO1Cas9, a cold-adapted Cas9 nuclease from the deep ocean, showing improved activity under cold conditions and broader organismal applicability.