Bacterial Genetics and Biotechnology

The paper shows that KorB, a DNA-sliding clamp, switches to a repressor role with KorA to mediate long-range gene silencing on plasmid RK2.

The paper investigates how the integrative and conjugative element ICEBs1 modulates the host SOS response to DNA damage in Bacillus subtilis.

The paper investigates the transmembrane signaling mechanism of the CitA histidine kinase using structural biology techniques and shows how structural changes in the sensor domain are transmitted across the membrane.

This study identifies the SpoVAF/FigP complex's role in Bacillus subtilis spore germination under moderate high pressure and examines factors affecting its function.

The paper identifies two temperature-responsive RNAs, CyaR and ompX, showing their cooperative role in regulating ompX expression through structural changes and RNA-RNA interactions at different temperatures.

The paper develops a co-evolution algorithm to predict bacterial iron interaction networks based on siderophore synthetase-receptor gene pairs across Pseudomonas genomes, revealing habitat- and pathogen-specific interactions.

The paper determines the cryo-EM structure of TraT proteins from plasmids and reveals their evolutionary history, showing independent acquisition by different plasmid backbones.

The paper investigates how variability in DnaA/DNA ratios contributes to phenotypic variability in E. coli by affecting replication initiation timing and nucleoid segregation.

The paper identifies two conserved residues in Hfq critical for stabilizing small regulatory RNAs under nitrogen starvation.

The paper shows RapA disrupts post-termination complexes in E. coli to prevent R-loop formation and maintain genome stability.

This study uses NMR and modeling to determine how MinC interacts with FtsZ, revealing that MinC caps FtsZ filaments to inhibit Z-ring formation.

The paper identifies two binding sites of the SinR*SlrR heteromer in the fla/che operon that cause premature termination, reducing SigD expression and motility in Bacillus subtilis.

The paper introduces ChIP-mini, a low-input ChIP-exo method, to study DNA-binding protein dynamics in Salmonella Typhimurium within macrophages, revealing H-NS and RpoD binding sites and their roles in virulence gene transcription.

The paper investigates the regulatory role of TezRs in Staphylococcus aureus gene transcription and identifies differentially expressed genes and pathways following TezR destruction.

This study shows that capsular polysaccharide in Acinetobacter baumannii reduces both its own and rival bacteria's T6SS efficacy, with increased CPS production exacerbating self-T6SS impairment.

The paper tracks ribosome localization during sporulation in B. subtilis and shows peptidoglycan rearrangement is essential for ribosome packing into the forespore.

This study identifies RbpB as a key RNA-binding protein regulating polysaccharide utilization in gut Bacteroides through post-transcriptional control of PULs.

This study identifies purine nucleotide limitation as a mechanism conferring antibiotic resistance in E. coli by reducing ATP supply and upregulating the purine nucleoside transporter PunC.

This study reveals the structural basis of RecA-LexA interaction in Pseudomonas aeruginosa, showing how RecA triggers LexA cleavage and inactivation, which is critical for the SOS response.

This study develops inducible conjugative plasmids for controlled horizontal gene transfer to combat antimicrobial resistance, showing increased conjugation efficiency upon induction.

This study investigates how transcription-translation coupling state affects termination in the E. coli galactose operon, showing that loop length determines terminator usage.

This study shows that adding trace elements to chemically defined media reduces variability in microbial growth experiments, supporting their routine use for more reproducible results.

This study identifies two distinct Type IV pili in Saccharibacteria, showing their roles in host binding, competition, and motility.

The paper identifies active promoters in Curvibacter sp. AEP1-3 using an oligonucleotide library and flow cytometry, revealing distinct temporal expression dynamics across growth phases.

This study visualizes peptidoglycan layers in gliding diderm bacteria using electron microscopy, revealing structural differences that may explain force transmission during locomotion.

The paper identifies a co-transformation artifact in Gateway cloning that challenges existing beliefs about plasmid maintenance and selection in E. coli.

The paper maps the evolution of flagellar motility across bacterial species by analyzing genome data and developing a classifier to predict motility based on protein homology.

The paper investigates how Bacteroides use the Y-Z regulatory system to control capsule polysaccharide (CPS) transcription and create subpopulations for fitness.

This study shows that in E. coli, the stringent response and polyP synthesis jointly regulate cell division, morphology, and metabolism under non-stress conditions.

The paper identifies a Pseudomonas aeruginosa-specific small molecule, BRD1401, that targets OprH and disrupts its interaction with LPS, increasing membrane fluidity.