Cellular transport and secretion

This paper identifies a novel PEX14/PEX5 interface involved in peroxisomal protein import and receptor recycling through biochemical and structural analysis.

This paper models clathrin-mediated endocytosis to show that the switch between productive and abortive CCPs is an emergent property of clathrin-membrane interactions and EAPs.

This study identifies Osbp and PI4KII as mutually dependent regulators of secretory granule maturation in Drosophila, showing their roles in PI4P enrichment and membrane tubule formation.

This study identifies suppressors of RDGB function in Drosophila and links them to novel lipids and ubiquitination processes in retinal degeneration and neurodevelopmental diseases.

This study shows that Mon1a and FCHO2 are required for maintaining Golgi architecture, with Mon1a interacting with dynein and FCHO2 forming lateral links between Golgi ministacks.

This study shows that deleting VPS50 in mice brains impairs synaptic function and behavior by disrupting V-ATPase pump localization and vesicle acidification.

This study identifies alternative cell entry mechanisms for SARS-CoV-2 and other viruses, showing some rely on dynamin while others use actin-dependent pathways.

This study identifies dAsap as a regulator of NMJ morphology and synaptic calcium homeostasis in Drosophila through Arf6-dependent signaling.

The paper reconstitutes core synaptic machinery to reveal DAG's role in vesicle priming and Ca2+-triggered release, showing DAG accelerates SNAREpin assembly and affects release dynamics.

The paper shows that MARCKS masks PIP2, arresting SNARE assembly and vesicle fusion, while PIP2 unmasking by Ca2+/CaM or PKC-epsilon rescues fusion and enhances synaptotagmin-1 activity.

The paper identifies GEFs and GAPs modulating Rab10 phosphorylation and abundance by LRRK2-G2019S in neurons, showing Crag acts as a GEF and plx as a conventional GAP.

This study investigates Arf6's role in angiogenesis by analyzing its impact on actin assembly and clathrin-mediated endocytosis in endothelial cells.

This study identifies TMEM87A as a novel Golgi-resident cation channel, GolpHCat, which regulates pH homeostasis and is essential for brain function and memory.

The paper shows that SV2A-Syt1 nanoclusters limit dynamin-1 recruitment, thereby controlling the re-entry of Syt1 into recycling synaptic vesicles.

The paper develops an automated assay to analyze Golgi morphology and single-cell transcriptomes, revealing that late endosomal pathways compensate for Golgi disruption caused by USO1 knockdown.

The paper investigates how Ca2+ and chelators like ATP and EGTA regulate the cis- and trans-membrane interactions of synaptotagmin-1 using purified vesicles and liposomes.

The paper identifies Fwd as a PI 4-kinase that regulates cell surface expansion during Drosophila epithelial morphogenesis, showing its role in apical constriction and tissue formation.

The paper quantifies the organization of the synaptic periactive zone (PAZ) and identifies relationships between endocytic proteins and active zone release properties in Drosophila synapses.

The paper shows that Dynamin 1xA and Syndapin 1 form liquid-like condensates at synapses, which facilitate ultrafast endocytosis by enabling molecular crowding and intermolecular interactions.

The paper shows that the Habc domain of syntaxin is required for Unc18 to open, enabling SNARE complex formation in synaptic transmission.

This study shows cholesterol is required for Ca2+-dependent vesicle fusion by stabilizing synaptotagmin-1-induced membrane bending.

The paper identifies SNARE and related vesicle trafficking factors in giant viruses, suggesting they may hijack host trafficking mechanisms for replication.

This study shows the exocyst complex facilitates multiple steps in SNARE complex assembly and vesicle fusion using purified yeast exocyst in reconstituted systems.

This study shows that the SV2A-Syt1 interaction forms surface nanoclusters, which regulate their entry into recycling synaptic vesicles.

The paper investigates active zone functions by knocking out RIM+ELKS and reconstituting key processes, showing that docking and fusion competence are separable and scaffolding is not required for release.

This study identifies a novel CCK complex involving CLN6, CRMP2, and KLC4 that regulates ER-derived vesicle transport in axons, with implications for neurodegenerative disease therapy.

The paper develops a kinetic model of vesicle fusion transitions that reproduces neuromuscular transmission dynamics, including facilitation and depression.

This study shows that high-fat diet feeding reduces Rab3a, Rab3gap1, and Rab3gap2 gene expression in the hypothalamus, potentially disrupting neuronal exocytosis and receptor recycling.

The paper shows Myosin-1b is essential for intestinal epithelial folding in zebrafish and contributes to villous atrophy when UNC45A is lost.

This study uses smFRET to show that syntaxin-3b's conformational changes regulate SNARE complex assembly in ribbon synapses, with phosphorylation promoting SNARE complex formation.