Force Microscopy Techniques and Applications

Light-guided molecular patterning for programmable multiplexed single-molecule manipulation

This paper introduces a light-guided surface patterning method for organizing oligonucleotides, enabling precise control and high-throughput single-molecule experiments.

High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell Level

This paper introduces a high-throughput centrifuge force microscope to quantify single-cell immune interactions, revealing receptor-specific binding dynamics and time-dependent avidity changes in BiTE-mediated T-cell binding.

AFMnanoSALQ: An Accurate Detection Framework for Semi-Automatic Labeling and Quantitative Analysis of α-Hemolysin Nanopores Using Intensity-Height Cues in HS-AFM Data

AFMnanoSALQ is a semi-automatic framework for labeling and quantifying α-hemolysin nanopores in HS-AFM data using intensity-height cues, offering fast deployment without training.

Scanning probe microscopy elucidates gelation and rejuvenation of biomolecular condensates

This paper uses scanning probe microscopy to study the mechanical properties of biomolecular condensates, revealing their gelation and rejuvenation processes.

A look beyond topography: transient phenomena of Escherichia coli cell division captured with high-speed in-line force mapping

This paper uses high-speed in-line force mapping to study dynamic mechanical properties of living E. coli during division, revealing transient phenomena like localized stiffening and membrane pore formation.

A hermetically closed sample chamber enables time-lapse nano-characterization of pathogenic microorganisms in-vitro

This paper introduces a hermetically sealed AFM sample chamber for safe, high-resolution time-lapse imaging of pathogenic microorganisms in vitro.

Co2+-mediated adsorption facilitates atomic force microscopy of DNA molecules at double-helix resolution

This paper introduces a Co2+-mediated adsorption method for DNA on mica to improve AFM imaging resolution by reducing salt precipitation compared to Ni2+.

An Effective Surface Passivation Assay for Single-Molecule Studies of Chromatin and Topoisomerase II

This paper describes a surface passivation assay to reduce non-specific adsorption for single-molecule studies of chromatin and topoisomerase II.

The Influence of Ionic Environment on Nucleosome-Mica Interactions Revealed via Molecular Dynamics Simulations

This study uses molecular dynamics simulations to show how potassium and magnesium ions affect nucleosome binding to mica surfaces, with potassium allowing stable binding with less structural change.

Engineering the Mechanical Stability of a Therapeutic Affibody/PD-L1 Complex by Anchor Point Selection

The paper investigates how anchor point selection affects the mechanical stability of an Affibody/PD-L1 complex using multiple experimental and computational methods.

Accurate Drift-Invariant Single-Molecule Force Calibration Using the Hadamard Variance

This paper introduces a drift-invariant force calibration method for multiplexed magnetic tweezers using Hadamard variance, showing higher precision and accuracy than existing techniques.

Single-molecule magnetic tweezers reveals that TAV2b-derived peptides underwind and stabilize double-stranded RNA

The paper uses single-molecule magnetic tweezers to study how TAV2b-derived peptides affect dsRNA structure and stability, finding they underwind and stabilize the duplex.

Force-Enhanced Sensitive Detection of New DNA-Interactive Agents from Microorganisms at the Single-Molecule Level

This paper introduces a single-molecule stretching assay to detect DNA-intercalating agents from microorganisms, identifying two strains and three compounds with anti-cancer activity.

In-Silico Analyses of Molecular Force Sensors for Mechanical Characterization of Biological Systems

This paper uses molecular dynamics simulations to analyze how design parameters affect the mechanical properties of three types of molecular force sensors.

Multiscale topological analysis of kinetoplast DNA via high-resolution AFM

This study uses high-resolution AFM and image analysis to analyze the topology of kinetoplast DNA, revealing structural properties and changes upon digestion.

Nonlinear compliance of NompC gating spring and its implication in mechanotransduction

The paper measures the mechanical properties of NompC gating springs and finds nonlinear compliance that may regulate mechanotransduction sensitivity.

Detecting genetic variation and base modifications together in the same single molecules of DNA and RNA at base pair resolution using a magnetic tweezer platform.

Supercoiling DNA locates mismatches

This study demonstrates a method using magnetic tweezers to detect single base pair mismatches in DNA by observing plectoneme pinning under specific conditions.