Monoclonal and Polyclonal Antibodies Research

The paper evaluates yeast-bacterial cell-cell interactions using flow cytometry and FACS to characterize nanobody-antigen binding.

NAb-seq provides a rapid, cost-effective, and species-independent method for sequencing full-length antibody genes from hybridoma cell lines and single B cells, outperforming Sanger sequencing in accuracy and throughput.

The paper develops a method to improve antibody thermostability by combining consensus sequence analysis with structural residue pair covariance from 3D structures.

The paper demonstrates antibody-mediated knockdown of intracellular protein expression to target specific protein variants, offering new therapeutic strategies for genotype-specific inhibition of pathogenic variants.

AbDiver enables comparison of therapeutic antibody sequences to natural antibody diversity, aiding in rational design by identifying mutation patterns and relevant sequence matches.

The paper develops nanobodies targeting the human transcobalamin-mediated vitamin B12 uptake route and shows their potential to inhibit cancer cell growth.

BioPhi provides automated tools for antibody humanization and humanness evaluation using deep learning and natural antibody repertoires, achieving results comparable to human experts.

The paper introduces two short NanoTag epitopes and their nanobodies for in vivo protein visualization and manipulation in Drosophila, demonstrating their utility in multiple assays and CRISPR-based tagging.

Llamanade is an open-source pipeline that humanizes nanobodies using sequence and structural analysis, improving their translational potential for therapeutic applications.

The paper identifies a high-homology light chain sequence from a hybridoma that is antigen-specific, highlighting challenges in variable domain sequencing.

This study demonstrates how ssDNA recombineering in bacteria enables hyper-diversification of nanobodies, resulting in variants that recognize new antigens while retaining original specificity.

The paper identifies and characterizes the diversity of antibody upstream sequences (AUS) across multiple species using Rep-Seq and 5RACE, revealing new variants and their implications for antibody biology.

The paper identifies and characterizes anti-TREM2 scFv antibodies that inhibit soluble TREM2 production.

This paper introduces light-controllable antibody mimetics, moonbody and sunbody, enabling rapid and reversible antigen recognition and precise control of cellular processes using light.

The paper analyzes antibody sequences from patents to assess their utility for therapeutic antibody engineering and finds they reflect clinical sequences.

The paper introduces a method to identify novel cross-clonotype antibodies by clustering based on predicted paratope features, validated on a Pertussis toxoid dataset.

The paper investigates how varying signal peptides and amino acid counts affect recombinant antibody production rates in HEK293 cells, developing a predictive model for transient production.

BASE is a software tool for analyzing single-cell immunoglobulin cloning data, providing automated analysis with high accuracy and additional plasmid sequence confirmation.

The paper investigates the interaction of rhTNF with Adalimumab and Etanercept, revealing new binding patterns and stoichiometry of their complexes.

AncesTree provides an interactive GUI for visualizing immunoglobulin lineage trees and mutations derived from Phylip analysis of antibody repertoires.

The paper develops PETAL, a library of 62,208 mAbs against 15,199 peptides, enabling proteome-scale antibody generation and target discovery across species.

This paper describes an engineered nanobody that modulates GFP binding with small molecules, enabling controlled study of GFP fusion proteins in biological processes.

The paper analyzes the structure and sequence of the antibody DE loop, revealing its role in antigen binding and its variability in HIV-1 antibodies.

cAb-Rep is a curated database of human B cell immunoglobulin sequence repertoires, providing annotated datasets for studying antibody diversity and prevalence.

The paper compares therapeutic antibodies to NGS-derived antibody sequences to identify sequence matches and convergence between natural and artificial antibody development.

The paper describes a method to generate and validate recombinant monoclonal antibodies for neuroscience research, enabling multiplex immunolabeling of brain tissue.

The paper introduces RANbodies, nanobody-reporter fusions that enable sensitive and scalable detection of molecules in cells and tissues.

This paper introduces a PCR-free NGS approach and software (DEAL) to accurately estimate antibody library diversity by accounting for sequencing errors.