Bacteriophages and microbial interactions

Insights into the spool-like architecture and infectionstrategy of the archaeal virus SEV1

This study uses cryo-electron tomography to reveal the spool-like architecture and infection strategies of the archaeal virus SEV1, showing its flexibility and unique assembly process.

Fold first, ask later: structure-informed function annotation of Pseudomonas phage proteins

This study uses structure-informed methods to annotate hypothetical proteins from Pseudomonas phages, showing that combining structural and sequence-based approaches can annotate up to 43% of previously unannotated proteins.

Arctic Ocean virus communities: seasonality, bipolarity, and prokaryotic interactions

The paper studies Arctic Ocean virus communities over four years, revealing seasonality, bipolar distribution, and interactions with prokaryotes.

Bacteriophage FNU1 negates Fusobacterium nucleatum induced cell growth and chemotherapy resistance in gastrointestinal cancer cells.

This study shows that bacteriophage FNU1 reduces Fusobacterium nucleatum-induced cancer cell growth and chemotherapy resistance in colon and gastric cancer models.

Nuclease-NTPase systems use shared molecular features to controlbacterial anti-phage defense

The paper analyzes 16 nuclease-NTPase systems to identify shared features controlling bacterial anti-phage defense, revealing universal protein complex formation and broad nucleic acid degradation capabilities.

Bacteriophage infection drives loss of β-lactam resistance in methicillin-resistant Staphylococcus aureus

This study shows that phage infection reduces β-lactam resistance and virulence in MRSA by altering gene expression and genetic profiles.

Negativeome in Early-Life Virome Studies: Characterization and Decontamination

The paper identifies viral contaminants in early-life gut viromes and shows the effectiveness of strain-aware decontamination to preserve biological signals.

The role and effects of the phage T4 Ac protein on infection

This study investigates the role of the T4 phage Ac protein in infection by showing it interacts with the AcrAB-TolC efflux pump, preventing acriflavine from hindering DNA replication.

Characterisation of a temperate phage induced from Alicyclobacillus acidoterrestris DSM 3922T: the Alicyclobacillus phage MMB025

This study isolates and characterizes a temperate phage, MMB025, that targets Alicyclobacillus acidoterrestris, showing its stability under various conditions and potential as a preservation strategy against food spoilage.

High-yield bioproduction of virus-free virus-like P4-EKORhE multi-lysin transducing particles as an antimicrobial gene therapeutic

This paper describes the construction and high-yield production of virus-like particles for delivering antimicrobial genes, demonstrating their effectiveness in bacterial cultures and human cell models.

A Novel Genus of Virulent Phage Targeting Acinetobacter baumannii: Efficacy and Safety in a Murine Model of Pulmonary Infection

This paper isolates and characterizes a novel virulent phage against Acinetobacter baumannii, showing efficacy in a murine model and safety in high-dose administration.

Phage-based microbiome manipulation elucidates interactions within community context

The paper uses phages to manipulate Variovorax species in microbiomes, confirming thiamine interdependencies and laying groundwork for targeted microbiome research.

Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDA{varphi}

This study presents the structure of the Neisseria meningitidis filamentous bacteriophage MDA{Phi}, revealing its organization and molecular mechanisms of biofilm formation and infection.

A TIR-SAVED effector mediates antiviral immunity via a conserved host signal

This study shows a TIR-SAVED effector can act independently as an antiviral defense, clearing viruses without its cyclase partner and using a host-produced second messenger.

Bacteria-phage (co)evolution is constrained in a synthetic community across multiple bacteria-phage pairs

This study shows that phage resistance evolution is inhibited in mixed bacterial communities compared to isolated pairs, highlighting the complexity of coevolution in natural systems.

Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance to Streptococcus mutans phage ΦAPCM01

This study identifies CRISPR spacers and RGP gene mutations in Streptococcus mutans conferring resistance to phage ΦAPCM01, with fitness costs and potential for cross-serotype infection.

Small viruses reveal bidirectional evolution between HK97-fold viruses and encapsulins via procapsids

The paper investigates the evolutionary relationship between HK97-fold viruses and encapsulins, finding bidirectional transitions through procapsids.

Phenotypic resistance to phage infection through capsule expression shutdown in Klebsiella pneumoniae

The paper shows that Klebsiella pneumoniae can resist phage infection through reversible downregulation of capsule biosynthesis without genetic mutations.

The complete genome and comparative analysis of a new Tequintavirus: Salmonella phage Tennessee Salten

The paper reports the genome analysis of a new Tequintavirus bacteriophage, Salmonella phage Tennessee Salten, and compares it to its closest relative, identifying unique and divergent genes.

Giant virus transcription and translation occur at well-defined locations within amoeba host cells

This study uses smFISH and other techniques to visualize Mimivirus transcription and translation sites in Acanthamoeba host cells, showing transcription occurs at defined spots and translation occurs in a ring around them.

High-resolution cryo-EM analysis of the therapeutic Pseudomonas phage Pa223

This paper presents a high-resolution cryo-EM structure of the Pseudomonas phage Pa223, revealing hybrid structural features shared with P22-like, T7-like, and Litunavirus phages.

Phage Steering in the Presence of a Competing Bacterial Pathogen

This study shows that Phage Steering with Luz19 can steer Pseudomonas aeruginosa to lose type IV pilus function even in the presence of Staphylococcus aureus.

Bacterial peptide deformylase inhibitors induce prophages in competitors

The paper shows that a metabolite from Vibrio ordalii induces prophages in competing Vibrio species by inhibiting peptide deformylase, enhancing competitive advantage through prophage activation.

Elucidating the mechanisms of action and evolutionary relationships of phage anti-defence proteins

The paper develops EVADES, a database of phage anti-defence proteins, predicts mechanisms for 21 uncharacterized ADPs, and explores their evolutionary and functional relationships.

PglZ from Type I BREX phage defence systems is a metal-dependent nuclease that forms a sub-complex with BrxB

This study identifies and characterizes the PglZ:BrxB sub-complex of BREX phage defence systems, revealing PglZ as a metal-dependent nuclease that contributes to phage defence.

Predicting clinical phage therapy outcomes in vitro: results using mixed versus single isolates from an MRSA case study

This study compares phage therapy outcomes using mixed versus single bacterial isolates from an MRSA case study, finding mixed isolates better predict lung infection outcomes than blood infection outcomes.

Enhancing Strain-level Phage-Host Prediction through Experimentally Vali-dated Negatives and Feature Optimization Strategies

This study improves strain-level phage-host prediction by using experimentally validated negatives and optimized features, showing better performance with protein-based features and experimental negatives.

Ultrastructural and transcriptional changes during a giant virus infection of a green alga

This study investigates the infection cycle of a giant virus in a green alga, documenting ultrastructural changes and transcriptional responses at different stages of infection.

Hidden viral players: Diversity and ecological roles of viruses in groundwater microbiomes

The paper analyzes groundwater viromes to show viruses influence microbial metabolism and nutrient cycling through auxiliary metabolic genes and host-targeting mechanisms.

Eliminating the type I restriction endonuclease from Pseudomonas aeruginosa PAO1 for optimised phage isolation

This paper removes the type I restriction endonuclease HsdR from Pseudomonas aeruginosa PAO1 to improve phage propagation efficiency and yield, enhancing phage isolation for clinical therapy.