A. K. Shukla

A genetically-encoded nanobody sensor reveals conformational diversity in beta-arrestins orchestrated by distinct seven transmembrane receptors

This study introduces a nanobody sensor to detect conformational changes in beta-arrestins upon GPCR activation, revealing receptor-specific conformational diversity.

Molecular basis of ligand promiscuity, structural mimicry, and atypical dimerization in the chemokine receptors

This study investigates ligand promiscuity and signaling bias in chemokine receptors by analyzing binding modes and determining cryo-EM structures of receptor-ligand complexes.

Structure of the human Duffy antigen receptor

This study determines the cryo-EM structure of the Duffy antigen receptor bound to CCL7, revealing structural features that explain its unique functional properties and lack of G protein coupling.

Molecular insights into atypical modes of beta-arrestin interaction with seven transmembrane receptors

This study presents cryo-EM structures and experimental data revealing how beta-arrestins interact with GPCRs, particularly those with phosphorylation in the third intracellular loop and intrinsically biased receptors.

Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution

This study presents cryo-EM structures of GPR109A-G-protein complexes with agonists and uses them to engineer biased agonists with reduced flushing effects.

pH - responsive, reversible A-motif based DNA hydrogels: synthesis and biosensing applications

This paper develops a pH-responsive A-motif DNA hydrogel that forms reversible structures and demonstrates its use in biosensing and cell surface detection.

Structural insights into agonist-binding and activation of the human complement C3a receptor

This study presents structural insights into C3a-C3aR interactions and identifies a G-protein-biased agonist for the receptor.

Structural insights into ligand-recognition, activation, and signaling-bias at the complement C5a receptor, C5aR1

This study presents cryo-EM structures of C5aR1 bound to C5a and a synthetic peptide, revealing conformational changes and G-protein coupling mechanisms.

Single-molecule analysis of receptor-beta-arrestin interactions in living cells

This study uses single-molecule microscopy and simulations to show that beta-arrestin inserts into the lipid bilayer and interacts with receptors through lateral diffusion, challenging existing models of its function.

A key GPCR phosphorylation motif discovered in arrestin2-CCR5 phosphopeptide complexes

The paper identifies a phosphorylation motif in arrestin2-CCR5 complexes that is essential for stable interactions and functional activation of CCR5.

Structural snapshots uncover a lock-and-key type conserved activation mechanism of β-arrestins by GPCRs

The paper presents structural snapshots of β-arrestins interacting with GPCRs, revealing a conserved 'lock-and-key' activation mechanism through specific phosphorylation patterns.

Molecular insights into intrinsic transducer-coupling bias in the CXCR4-CXCR7 system

This study characterizes the biased agonism of CXCR4 and CXCR7, revealing differences in G-protein coupling, β-arrestin recruitment, and ERK1/2 activation.

Allosteric modulation of GPCR-induced β-arrestin trafficking and signaling by a synthetic intrabody

This paper shows that a synthetic intrabody enhances β-arrestin endosomal trafficking and ERK1/2 activation in a GPCR mutant by promoting an active-like conformation.

Intrinsic bias at non-canonical, β-arrestin-coupled seven transmembrane receptors

The paper identifies two non-canonical GPCRs, D6R and C5aR2, that exclusively couple to β-arrestins and exhibit distinct GRK preferences and downstream signaling.

Crystal structure of β-arrestin 2 in complex with an atypical chemokine receptor phosphopeptide reveals an alternative active conformation

The paper presents a crystal structure of β-arrestin 2 bound to a phosphopeptide from ACKR3, revealing an alternative active conformation distinct from β-arrestin 1.

Purification of native CCL7 and its functional interaction with selected chemokine receptors

This study describes a method to purify native CCL7 and its isotopically labeled derivatives, enabling structural and functional studies of chemokine receptor interactions.

Conformational sensors and domain-swapping reveal structural and functional differences between β-arrestin isoforms

The paper identifies structural differences between β-arrestin isoforms using conformational sensors and domain-swapping, linking these differences to their distinct functional outcomes in GPCR signaling.

Genetically encoded intrabody sensors illuminate structural and functional diversity in GPCR-β-arrestin complexes

This study uses genetically encoded intrabody sensors to reveal structural and functional diversity in GPCR-β-arrestin complexes, showing distinct functional outcomes across different receptors.

Partial ligand-receptor engagement yields functional bias at the human complement receptor, C5aR1

This study investigates ligand bias at C5aR1 by comparing C5a and C5apep, showing differential activation of G proteins, βarr recruitment, endocytosis, and ERK1/2 phosphorylation.