Author

C.-L. Hsieh

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Immunology· 7 Microbiology· 4 Biophysics· 3 Biochemistry· 1 Cell Biology· 1 Plant Biology· 1

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Approach:experimental assay· 14 Research Focus:host-pathogen & immune response· 8 Organism:human· 7 Assay:proteomics· 7 Research Focus:drug response / drug discovery· 3 Research Focus:cell signaling & trafficking· 2 Research Focus:cell type annotation· 2 Assay:cryo-EM· 2

Frequent Co-Authors

J. S. McLellan· 10 shared D. Wrapp· 4 shared A. W. Nguyen· 3 shared J. A. Maynard· 3 shared N. V. Johnson· 3 shared N. Wang· 3 shared A. C. Walls· 2 shared A. M. DiVenere· 2 shared

Activity by Year

2018-2023 · peak 6/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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10 shared papersImmunology

J. S. McLellan

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17 papers

Recent·Most cited

Structural and functional analyses of viral H2 protein of the vaccinia virus entry fusion complex

C.-F. Kao, C.-Y. Liu, C.-L. Hsieh, K. J. D. Carillo, D.-L. M. Tzou, H.-C. Wang, W. Chang

This study determines the structure and function of the H2 protein in the vaccinia virus entry fusion complex, identifying key regions involved in membrane fusion and complex formation.

bioRxiv · Aug 28, 2023Microbiology

Pannexin 1 modulates angiogenic activities of human endothelial colony-forming cells through IGF-1 mechanism and is a marker of senescence

H.-I. Yeh, T.-Y. Tien, Y.-J. Wu, C.-H. Su, C.-L. Hsieh, B.-J. Wang, Y.-N. Lee, Y. Su

This study shows that reducing Panx1 in senescent endothelial colony-forming cells increases IGF-1 production, improving angiogenic activity and limb perfusion in mice.

bioRxiv · May 1, 2023Cell Biology

Identification of a conserved neutralizing epitope present on spike proteins from all highly pathogenic coronaviruses

Y. Huang, A. W. Nguyen, C.-L. Hsieh, R. Silva, O. S. Olaluwoye, R. Wilen, T. S. Kaoud, L. R. Azouz, A. N. Qerqez, K. C. Le, A. L. Bohanon, A. M. DiVenere, Y. Liu, D. Amengor, S. R. Shoemaker, , J. A. Maynard

The paper identifies a conserved neutralizing epitope on the S2 domain of SARS-CoV, SARS-CoV-2, and MERS-CoV spike proteins that is targeted by antibody 3A3.

eLife · Mar 21, 2023Peer-reviewedBiochemistry

Amis Pacilo and Yami Cipoho are not the same as the Pacific breadfruit starch crop--Target enrichment phylogenomics of a long-misidentified Artocarpus species sheds light on the northward Austronesian migration from the Philippines to Taiwan

C.-R. Chuang, C.-L. Hsieh, C.-S. Chang, C.-M. Wang, D. N. Tandang, E. M. Gardner, L. Audi, N. J. C. Zerega, K.-F. Chung

bioRxiv · Jul 28, 2022Plant Biology

Characterization of prefusion-F-specific antibodies elicited by natural infection with human metapneumovirus

S. A. Rush, G. Brar, C.-L. Hsieh, E. Chautard, J. N. Rainho-Tomko, C. Slade, C. A. Bricault, A. Kume, J. Kearns, R. Groppo, S. Mundle, L. Zhang, D. Casimiro, T.-M. Fu, J. M. DiNapoli, J. S. McLellan

This study characterizes prefusion-F-specific antibodies from elderly individuals naturally infected with hMPV, revealing their epitope recognition and neutralizing activity.

bioRxiv · Mar 28, 2022Immunology

Structural basis for ultrapotent neutralization of human metapneumovirus

A. Banerjee, J. Huang, S. Rush, J. Murray, A. D. Gingerich, F. Royer, C.-L. Hsieh, R. A. Tripp, J. S. McLellan, J. J. Mousa

This study identifies a potent monoclonal antibody against hMPV and determines its structure, revealing a novel epitope for therapeutic development.

bioRxiv · Mar 14, 2022 · PreprintImmunology

The SARS-CoV-2 spike reversibly samples an open-trimer conformation exposing novel epitopes

S. M. Costello, S. R. Shoemaker, H. T. Hobbs, A. W. Nguyen, C.-L. Hsieh, J. A. Maynard, J. S. McLellan, J. E. Pak, S. Marqusee

The paper identifies an alternative open-trimer conformation of the SARS-CoV-2 spike ectodomain that exposes novel epitopes and is modulated by temperature, binding, and sequence variants.

bioRxiv · Jul 11, 2021Biophysics

Potent neutralization of SARS-CoV-2 variants of concern by an antibody with a unique genetic signature and structural mode of spike recognition

K. J. Kramer, N. V. Johnson, A. R. Shiakolas, N. Suryadevara, S. Periasamy, N. Raju, J. K. Williams, D. Wrapp, S. J. Zost, C. M. Holt, C.-L. Hsieh, R. E. Sutton, A. Paulo, E. Davidson, B. J. Doranz, , I. S. Georgiev

This study identifies a potent SARS-CoV-2 antibody, 54042-4, that neutralizes variants of concern and has a unique epitope and genetic structure.

bioRxiv · May 16, 2021Immunology

Early cross-coronavirus reactive signatures of protective humoral immunity against COVID-19

P. Kaplonek, C. Wang, Y. Bartsch, S. Fischinger, M. J. Gorman, K. Bowman, J. Kang, D. Dayal, P. Martin, R. Nowak, C.-L. Hsieh, J. Feldman, B. Juelg, E. Nilles, E. R. Musk, , G. Alter

The paper profiles early humoral immune responses in COVID-19 patients, finding that survivors show rapid SARS-CoV-2-specific immunity linked to pre-existing OC43 immunity, while non-survivors have delayed and blunted responses.

bioRxiv · May 11, 2021 · PreprintImmunology

Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccines

A. C. Walls, M. C. Miranda, M. N. Pham, A. Schaefer, A. Greaney, P. S. Arunachalam, M.-J. Navarro, M. A. Tortorici, K. Rogers, M. A. O'Connor, L. Shireff, D. E. Ferrell, N. Brunette, E. Kepl, J. E. Bowen, , Veesler

This study shows that a SARS-CoV-2 RBD nanoparticle vaccine protects against SARS-CoV-2 and related sarbecoviruses, including variants, in mice and non-human primates.

bioRxiv · Mar 15, 2021Immunology

Adjuvanting a subunit SARS-CoV-2 nanoparticle vaccine to induce protective immunity in non-human primates

P. S Arunachalam, A. C. Walls, N. Golden, C. Atyeo, S. Fischinger, C. Li, P. Aye, M. J. Navarro, L. Lai, V. V. Edara, K. Roltgen, K. Rogers, L. Shirreff, D. E. Ferrell, S. Wrenn, , Coffma

This study evaluates adjuvants for a subunit SARS-CoV-2 nanoparticle vaccine in non-human primates, showing durable neutralizing antibody responses and protection against infection.

bioRxiv · Feb 10, 2021 · PreprintImmunology

D614G Substitution of SARS-CoV-2 Spike Protein Increases Syncytium Formation and Viral Transmission via Enhanced Furin-mediated Spike Cleavage

Y.-W. Cheng, T.-L. Chao, C.-L. Li, S.-H. Wang, H.-C. Kao, Y.-M. Tsai, H.-Y. Wang, C.-L. Hsieh, P.-J. Chen, S.-Y. Chang, S.-H. Yeh

This study shows the D614G substitution in SARS-CoV-2 spike protein increases viral transmission by enhancing furin-mediated cleavage, leading to more syncytium formation and higher virus titers.

bioRxiv · Jan 27, 2021Microbiology

Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes

C.-L. Hsieh, J. A. Goldsmith, J. M. Schaub, A. M. DiVenere, H.-C. Kuo, K. Javanmardi, K. C. Le, D. Wrapp, A. G.-W. Lee, Y. Liu, C.-W. Chou, P. O. Byrne, C. K. Hjorth, N. V. Johnson, J. Ludes-Meyers, , J. S. McLellan

This study designs and characterizes prefusion-stabilized SARS-CoV-2 spike variants to improve protein yield and stability for vaccine and diagnostic development.

bioRxiv · May 30, 2020Microbiology

Broad sarbecovirus neutralizing antibodies define a key site of vulnerability on the SARS-CoV-2 spike protein

A. Z. Wec, D. Wrapp, A. S. Herbert, D. P. Maurer, D. Haslwanter, M. Sakharkar, R. K. Jangra, M. E. Dieterle, A. Lilov, D. Huang, L. V. Tse, N. V. Johnson, C.-L. Hsieh, N. Wang, J. H. Nett, , L. M. Walker

The paper identifies broadly neutralizing antibodies against SARS-CoV-2 and related sarbecoviruses, highlighting a conserved epitope on the spike protein as a potential vaccine target.

bioRxiv · May 15, 2020 · PreprintImmunology

Cryo-EM Structure of the 2019-nCoV Spike in the Prefusion Conformation

D. Wrapp, N. Wang, K. S. Corbett, J. A. Goldsmith, C.-L. Hsieh, O. Abiona, B. S. Graham, J. S. McLellan

The paper determines the cryo-EM structure of the 2019-nCoV Spike protein in the prefusion conformation and shows higher ACE2 binding affinity compared to SARS-CoV.

Science · Mar 13, 2020Peer-reviewedMicrobiology

Characterization of Single Protein Dynamics in Cell Plasma Membrane Derived Polymer Cushioned Lipid Bilayers

W. C. Wong, J.-Y. Juo, Y.-H. Liao, C.-Y. Cheng, C.-H. Lin, C.-L. Hsieh

This study characterizes single membrane protein dynamics in polymer-cushioned lipid bilayers and finds that increasing polymer length enhances protein mobility and reduces transient confinement.

bioRxiv · May 17, 2019 · PreprintBiophysics

The Organization of Membranes Below 100 nm: Experimental Breakthroughs Occasion a Modeling Manifesto

E. Lyman, C. Eggeling, C.-L. Hsieh

The paper reviews experimental advances revealing membrane heterogeneity and scale dependence, and calls for simulation methods that can capture these features to better understand membrane organization.

bioRxiv · Mar 31, 2018Biophysics