R. A. Bonomo

{Omega}-Loop mutations control dynamics of the active site by modulating the hydrogen-bonding network in PDC-3 β-lactamase

This study investigates how {Omega}-loop mutations in PDC-3 β-lactamase affect its active site dynamics and catalytic activity through molecular simulations.

Hetero-antagonism of avibactam and sulbactam with cefiderocol in carbapenem-resistant Acinetobacter spp.

This study investigates the molecular mechanisms of cefiderocol resistance and antagonism with avibactam and sulbactam in carbapenem-resistant Acinetobacter strains.

Deciphering the co-evolutionary dynamics of L2 β-lactamases via Deep learning

This paper uses deep learning to analyze the co-evolutionary dynamics of L2 β-lactamases and their functional mechanisms.

Synergistic Effects of Sulopenem in Combination with Cefuroxime or Durlobactam against Mycobacterium abscessus

This study shows that sulopenem combined with cefuroxime or durlobactam synergistically inhibits Mycobacterium abscessus, with BLIs enhancing antibacterial effects.

Evolution under vancomycin selection drives divergent collateral sensitivity patterns in Staphylococcus aureus

This study evolves Staphylococcus aureus under vancomycin selection, revealing two distinct adaptive pathways that lead to divergent collateral sensitivity patterns to first-line antibiotics.

The Structural Role of N170 in Substrate-assisted Deacylation in KPC-2 β-Lactamase

This study investigates the structural role of N170 in substrate-assisted deacylation in KPC-2 β-lactamase, revealing its impact on drug binding and catalytic activity.

Gating interactions steer loop conformational changes in the active site of the L1 metallo-β-lactamase

The paper investigates how gating interactions between loops in the L1 metallo-β-lactamase affect active site conformational changes and identifies key residues critical for enzyme activity.

A low-cost, open-source evolutionary bioreactor and its educational use

The paper introduces a low-cost, open-source morbidostat for educational use, validated through bacterial resistance experiments and student engagement.

The Iron Content of Human Serum Albumin Modulates the Susceptibility of Acinetobacter baumannii to Cefiderocol

This paper shows that human serum albumin (HSA) increases Acinetobacter baumannii resistance to cefiderocol by modulating iron uptake and beta-lactam resistance gene expression.

Carbapenem-Resistant Acinetobacter baumannii in US hospitals: diversification of circulating lineages and antimicrobial resistance

This study analyzes CRAb isolates from US hospitals using WGS, identifying new sub-lineages and resistance patterns, highlighting shifts in CRAb population structure and clinical impact.

Impact of Human Serum Proteins on Susceptibility of Acinetobacter baumannii to Cefiderocol: role of iron transport

The paper shows that human serum and its components increase Acinetobacter baumannii resistance to cefiderocol by altering iron uptake and beta-lactam resistance genes.

Interplay between meropenem and human serum albumin on expression of carbapenem resistance genes and natural competence in Acinetobacter baumannii

This study shows that human serum albumin and meropenem together enhance natural transformation and gene expression in Acinetobacter baumannii, potentially promoting resistance and evolution.

Development of antibiotic resistance reveals diverse evolutionary pathways to face the complex and dynamic environment of a long-term treated patient

This study tracks the long-term evolution of antibiotic-resistant Pseudomonas aeruginosa in a cystic fibrosis patient, revealing diverse genetic changes that enhance resistance to multiple cephalosporins.

Cerebrospinal fluid (CSF) boosts metabolism and virulence expression factors in Acinetobacter baumannii

This study shows that cerebrospinal fluid (CSF) enhances virulence factor expression and metabolic processes in low-virulence Acinetobacter baumannii strains.

Allosteric communication in Class A β-lactamases occurs via Cooperative Coupling of Loop Dynamics

This study identifies allosteric communication pathways in Class A β-lactamases using molecular dynamics simulations, showing how amino acid substitutions affect enzyme activity and antibiotic resistance.

The H-NS regulator plays a role in the stress induced by carbapenemase expression in Acinetobacter baumannii

This study shows H-NS alleviates envelope stress from MBL expression and DNA-damaging agents in A. baumannii, linking it to stress response and carbapenem resistance.

A non-canonical metal center drives activity of the Sediminispirochaeta smaragdinae metallo-β- lactamase SPS-1

This study characterizes the metallo-β-lactamase SPS-1 from Sediminispirochaeta smaragdinae, revealing a novel metal binding site that challenges inhibitor design.

Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain

This study shows that natural transformation enables Acinetobacter baumannii to acquire antibiotic resistance genes from Klebsiella pneumoniae, contributing to the emergence of multi-drug-resistant strains.