R. J. Coffey

miR-100 and miR-125b Contribute to Enhanced 3D Growth and Invasiveness and can be Functionally Transferred to Silence Target Genes in Recipient Cells

This study identifies miR-100 and miR-125b as regulators of 3D growth and invasiveness in cancer cells, showing they can be transferred extracellularly to silence target genes in recipient cells.

Transcriptomic Profiling Reveals Claudin 18.2 as a Diagnostic Biomarker of Menetrier Disease and the Role of Hedgehog Signaling in Pathogenesis

This study identifies CLDN18.2 as a diagnostic marker for Menetrier Disease and shows that Hedgehog signaling is upregulated and therapeutically targetable in the disease.

Detection of EGFR and its Activity State in Plasma CD63-EVs from Glioblastoma Patients: Rapid Profiling using an Anion Exchange Membrane Sensor

This paper introduces a novel immunoassay for detecting EGFR and its activity state in plasma CD63-EVs from glioblastoma patients, achieving high sensitivity and specificity for early detection.

Golgi organization is a determinant of stem cell function in the small intestine

This study shows that Lgr5+ stem cells in the small intestine adjust their Golgi apparatus orientation to enhance niche signal reception and tissue regeneration, which is impaired with age.

Inhibition of the BMP pathway suppresses tumor growth via downregulation of EGFR in MEK/ERK-dependent colorectal cancer

This study shows that inhibiting the BMP pathway suppresses colorectal cancer growth by reducing EGFR, and combining BMP and MEK inhibition enhances therapeutic effects in patient-derived organoids.

Lrig3 restricts the size of the colon stem cell compartment

This study shows that Lrig3 limits the size of the colon stem cell compartment by regulating cell number and crypt structure in mice.

Human colorectal pre-cancer atlas identifies distinct molecular programs underlying two major subclasses of pre-malignant tumors

The paper creates a single-cell atlas of colorectal pre-cancerous polyps, identifying distinct molecular programs in adenomas and sessile serrated lesions and their associations with CRC subtypes.

Murine intrarectal instillation of purified recombinant C. difficile toxins enables mechanistic studies of pathogenesis

This study uses purified recombinant C. difficile toxins in a mouse model to investigate their pathogenic mechanisms and tissue effects.

Clinically adaptable polymer enables simultaneous spatial analysis of colonic tissues and biofilms

This paper introduces a clinically adaptable polymer that preserves spatial compartments of colonic tissues and biofilms for simultaneous analysis.