Seth Blackshaw

The paper identifies mechanisms that generate M/L cone dominance and suppress S cones in the human foveola using retinal organoids and signaling pathway regulators.

This study shows Lhx2 modulates Sonic Hedgehog signaling in retinal progenitors through direct and indirect regulation of pathway components, establishing competence for signaling during early neurogenesis.

This study identifies Pantr2 as a trans-acting lncRNA that regulates gene expression and chromatin accessibility, affecting neural progenitor differentiation through Notch and Wnt signaling disruption.

This paper introduces SLED, a splicing-based method to control gene expression in specific cell types, demonstrating its utility in targeting neurons, photoreceptors, and cancer cells.

This study shows that GFAP promoter-driven AAV constructs in adult mouse retina show insert-dependent expression in non-glial cells without evidence of glia-to-neuron conversion.

This study transplants human retinal organoids into mice and identifies two classes of graft-derived cells with distinct migration and maturation behaviors.

This study shows that NFkB signaling suppresses Müller glia-mediated neuron regeneration in the mammalian retina by promoting immune cell accumulation and inhibiting neurogenic potential.

The paper identifies cell-class-specific cis-regulatory elements in human retina and organoids, showing their correlation and function in development and disease.

This study investigates the role of fatty acid-binding proteins (FABPs) and fatty acid synthase (FASN) in glial reactivity and Müller glia-derived progenitor cell formation in the chick retina using scRNA-seq.

LRLoop identifies bidirectional ligand-receptor feedback loops in cell-cell communication, reducing false positives and revealing new interactions in retinal development.

IReNA integrates scRNA-seq and scATAC-seq data to infer regulatory networks, outperforming existing methods in identifying known regulators.

This study uses single-cell RNA-Seq to analyze early chick hypothalamic development, revealing that hypothalamic cells originate from prethalamic-like progenitors and identifying novel molecular regulators.

This study shows that NrCAM regulates adult hypothalamic tanycytes, affecting their numbers and marker expression, but with only subtle metabolic consequences.

This study identifies gene regulatory networks controlling retinal development by integrating single-cell RNA and ATAC-seq data from mouse and human retinas, revealing transcription factors that regulate temporal patterning and cell fate.

The paper identifies stage-specific microglia subtypes in Alzheimer's disease using single-cell RNA-sequencing, showing both amyloid-beta and tau pathologies are required for their induction.

This study shows that deleting Ptbp1 in adult retinal Muller glia and brain astrocytes does not convert glia into neurons, using genetic lineage tracing, scRNA-Seq, and electrophysiology.

The paper uses single-cell RNA-Seq to map transcriptional programs in synovial joint development, identifying a progenitor population and the role of FGF signaling in chondrocyte fate.

This study identifies NFI factors as repressors of tanycyte-derived neurogenesis and shows their disruption promotes proliferation and neurogenesis in the hypothalamus.

The paper uses transcriptomic analysis to compare gene expression in control and TAO patients' orbital fat and differentiating orbital fibroblasts, identifying IGF-1R and Wnt signaling pathway differences.

This study investigates the role of the endocannabinoid system in reprogramming Muller glia into progenitor cells in the retina, showing that eCB signaling promotes this process while suppressing NFkB activity in mouse Muller glia.

This study shows that Atoh7 is essential for RGC survival but not for their specification, as RGCs can develop without Atoh7 when apoptosis is inhibited.

This study investigates MDK's role in retinal development and regeneration in chick and mouse, showing its impact on neuronal survival, glial reactivity, and Müller glia-derived progenitor cell formation.

The paper identifies two developmental origins of thalamic inhibitory interneurons in mice and shows their distribution in the marmoset thalamus.

This study identifies molecular mechanisms controlling the development of hypothalamic GABAergic neurons expressing Lhx6, showing their distinct regulatory networks compared to cortical interneurons.

The paper characterizes mWake expression in the adult mouse brain, identifying its presence in neurons and non-neuronal cells across multiple brain regions and providing single-cell RNA sequencing analysis of hypothalamic mWake+ cells.

This study identifies gene regulatory networks controlling proliferative and neurogenic competence in retinal Muller glia.

The paper investigates the role of somatostatin signaling via Sstr2 in retinal neurogenesis using scRNA-Seq and in vivo experiments, finding it inhibits photoreceptor generation and affects progenitor dynamics.

The paper constructs a single-cell transcriptional atlas of synovial joint progenitors from E12.5 to E15.5 in mice to uncover transcriptional programs and cell interactions during joint development.

The paper uses scRNA-Seq to analyze mouse hypothalamic development, identifying gene expression trajectories and regional subdivisions, and reveals Nkx2.1 as a negative regulator of prethalamic identity.