Aryeh Warmflash

Optogenetic BMP4 models symmetry breaking and primitive streak formation in hPSCs

This paper introduces LightBMP, an optogenetic tool to control BMP4 in hPSCs, showing spatial BMP4 patterns can drive symmetry breaking and primitive streak formation without mechanical cues.

Primate lineage specification requires suppression of Alu hyperediting

The paper identifies ILF2/3 as a regulator of primate cell fate by suppressing Alu element editing, showing its role in preventing aberrant splicing and maintaining epigenetic stability.

Comparison between the activities of canonical Wnt ligands in human pluripotent stem cell differentiation

The paper compares the activities of canonical Wnt ligands in human pluripotent stem cell differentiation and finds differences in their ability to induce mesodermal and ectodermal markers.

Juxtaposition of human pluripotent stem cells with amnion-like cells is sufficient to trigger primitive streak formation

This study shows that juxtaposing amnion-like cells with human pluripotent stem cells initiates gastrulation behaviors, including primitive streak formation and mesendodermal differentiation.

A novel self-organizing human pluripotent stem cell system reveals the role of Wnt signaling parameters in anterior-posterior patterning of the nervous system

This study develops an in vitro model to investigate how Wnt signaling dynamics influence anterior-posterior patterning in human neuroectoderm.

Dependence of cell fate potential and cadherin switching on primitive streak coordinate during differentiation of human pluripotent stem cells

This study shows that cadherin switching during mesendoderm differentiation from human pluripotent stem cells is independent of cell fate commitment, with distinct signaling dependencies.

Endogenous Nodal switches Wnt interpretation from posteriorization to germ layer differentiation in geometrically constrained human pluripotent cells

This study shows that endogenous Nodal signaling switches Wnt's effect from posteriorization to germ layer differentiation in human pluripotent cells under geometric constraints.

Combinatorial interpretation of BMP and WNT allows BMP to act as a morphogen in time but not in concentration

The paper shows that BMP signaling in human pluripotent stem cells induces mesodermal differentiation through timed pulses rather than concentration, involving combinatorial interactions with WNT signaling.

Nodal is a short-range morphogen with activity that spreads through a relay mechanism in human gastruloids

The paper shows Nodal is a short-range morphogen in human gastruloids, spreading via a relay mechanism and regulated by Lefty.

Self-organized morphogenesis of a human neural tube in vitro by geometric constraints

This paper describes an in vitro method to self-organize a human neural tube using geometric constraints, showing that neural and epidermal tissues are sufficient for morphogenesis.

BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

The paper compares BMP-treated human embryonic stem cells to amnion cells in monkey embryos, finding they are more transcriptionally similar to amnion cells than trophectoderm cells.

Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning

The paper introduces a rapid hydrogel micropatterning method using projection stereolithography to study self-organized developmental patterning of human embryonic stem cells.

Quantifying cell transitions in C. elegans with data-fitted landscape models

The paper develops a data-fitted landscape model for C. elegans vulval development using Catastrophe Theory and ABC, revealing a two-step decision process controlled by EGF and Notch-Delta signals.

Mesenchymal-Epithelial Transition Regulates Initiation of Pluripotency Exit before Gastrulation

The paper shows that pluripotent stem cells undergo a mesenchymal-epithelial transition (MET) before losing pluripotency, which is distinct from the gastrulation EMT and regulated by integrin-mediated cell-matrix interactions.

Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids.

The paper shows BMP signaling initiates WNT, which initiates NODAL in human gastruloids, revealing dynamic signaling waves that mediate epiblast patterning without a signaling gradient.

Synergy with TGFβ ligands switches WNT pathway dynamics from transient to sustained during human pluripotent cell differentiation

The paper investigates how TGFβ ligands alter WNT/{beta}-catenin signaling dynamics during human pluripotent cell differentiation, showing a shift from transient to sustained responses.

A novel self-organizing embryonic stem cell system reveals signaling logic underlying the patterning of human ectoderm

This paper develops an in vitro model of human ectodermal patterning using hESCs that self-organize into patterns mimicking the dorsal-ventral axis, revealing the role of WNT and BMP signaling in fate decisions.

Morphogen dynamics control patterning in a stem cell model of the human embryo

This study shows that morphogen dynamics, not just concentration, control cell fate decisions in human embryonic stem cells during gastrulation.

Morphogen And Community Effects Determine Cell Fates In Response To BMP4 Signaling In Human Embryonic Stem Cells

The paper shows that BMP4 signaling in hESCs leads to single cell fates above a threshold in colonies, while requiring secondary signals and specific densities in standard conditions, with community effects enhancing response to external signals.