Florian Wilfling

Quality control at the Golgi couples membrane proteostasis to lipid metabolism

The paper identifies a Golgi quality control checkpoint in yeast that links membrane proteostasis to lipid metabolism, showing that lipid imbalance from proteostasis defects is lethal.

Lipid versatility of the C2C domain of Extended Synaptotagmin 3 safeguards plasma membrane binding

The paper investigates the membrane-binding mechanism of the C2C domain of E-Syt3, revealing its lipid versatility and how it maintains ER-PM contacts during PI(4,5)P2 depletion.

Probing intracellular physical environments by rotational and translational single-particle tracking

This paper introduces a method combining rotational and translational single-particle tracking to analyze nanoscale dynamics, showing it reveals distinct biophysical features of cellular compartments.

A human lysosomal storage disorder toolkit for decoding proteome landscapes in cortical and dopaminergic-like induced neurons

The paper develops a human stem cell toolkit for studying lysosomal storage disorders by generating proteomic data from induced cortical and dopaminergic-like neurons.

Cathepsin-dependent amyloid formation drives mechanical rupture of lysosomal membranes

This study shows that LLOMe forms amyloid structures inside lysosomes that mechanically rupture the lysosomal membrane, revealing a structural mechanism for lysosomal damage.

Biomolecular condensate architecture of an autophagic cargo at molecular resolution in situ

The paper determines the molecular structure of autophagic cargo condensates using cryo-electron tomography and shows how disordered regions influence condensate properties.

The small GTPase Ran defines Nuclear Pore Complex asymmetry

This study shows that the small GTPase Ran controls the asymmetry of nuclear pore complexes by regulating their peripheral configuration in budding yeast and Drosophila.

In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment

Cryo-ET of Parkin-expressing U2OS cells shows oligomycin/antimycin depolarization moves some ATP synthase to the inner boundary membrane, abolishes matrix calcium granules, and favors closed prohibitin domes.

Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST

This study uses nMOST to profile proteo-lipidomics in lysosomal storage disease mutants, revealing autophagy defects and mitochondrial dysfunction.