W. Lee Kraus

PARP16 is a Druggable Regulator of Ribosome MARylation and Protein Homeostasis in Ovarian Cancer Cells

This study investigates PARP16's role in ovarian cancer by showing that inhibiting it with DB008 disrupts ribosome MARylation, impairs protein homeostasis, and reduces tumor growth.

Systematic CRISPRi perturbation of 1,408 autism risk genes maps multilevel transcriptional convergence in human cortical neurons

This study uses CRISPRi Perturb-seq to analyze 1,408 autism risk genes in human cortical neurons, identifying 215 genes that cause global transcriptomic dysregulation and uncovering shared transcriptional pathways.

Regulatory network hubs guide dynamic human lineage specification

The paper identifies dynamic regulatory network hubs in human lineage specification using Perturb-Seq and constructs a deep learning model to predict perturbed TFs in patient transcriptomes.

Mapping the Subtype-Specific PARP1 ADP-ribosylated Proteome in Breast Cancer Cells

The paper maps PARP1 ADP-ribosylated proteomes in breast cancer subtypes, identifying subtype-specific modifications and their functional implications.

Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells

This study benchmarks and optimizes Perturb-seq in human pluripotent stem cells, identifying shared regulatory networks during cardiomyocyte differentiation.

Estrogen Receptor Enhancers Sensitive to Low Doses of Hormone Specify Distinct Molecular and Biological Outcomes

The paper examines the molecular effects of varying estradiol doses on estrogen receptor enhancers in breast cancer cells, finding distinct biological outcomes at low versus high doses.

Role of Macrophage PARP1 in the Regulation of Crosstalk between Adipose Immune Cells and Adipocytes during Diet-induced Obesity

This study shows that macrophage PARP1 depletion disrupts adipose tissue homeostasis by enhancing cell-cell crosstalk, leading to obesity and metabolic dysfunction.

Phenotypic and Functional Characterization of Oncohistone Mutations in Breast Cancers

This study identifies and characterizes 17 high frequency oncohistone mutations in breast cancer that promote cancer-related phenotypes and may act as potential cancer drivers.

NAD+ Sensing by PARP7 Regulates the C/EBPβ-Dependent Transcription Program in Adipose Tissue In Vivo

This study identifies PARP7 as a key regulator of C/EBPβ-dependent adipogenesis and shows it senses nuclear NAD+ levels to control gene expression and lipid production in mammary glands.

X-Linked Cancer-Associated Polypeptide (XCP) from lncRNA1456 Cooperates with PHF8 to Regulate Gene Expression and Cellular Pathways in Breast Cancer

This study identifies XCP, a protein from the lncRNA1456, which interacts with PHF8 to regulate gene expression and cancer pathways in breast cancer.

SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis

The paper identifies SERBP1 as a protein involved in PARylation-dependent complexes that regulate splicing, cell division, and ribosome biogenesis, with implications for neurodegenerative diseases.

Intracellular Retention of Estradiol is Mediated by GRAM Domain Containing Protein ASTER-B in Breast Cancer Cells

This study identifies ASTER-B as a mediator of intracellular estradiol retention in breast cancer cells, which supports estrogen receptor-dependent gene expression and cell growth.

Relaxin Modulates the Genomic Actions and Biological Effects of Estrogen in the Myometrium by Reducing Estrogen Receptor Alpha Phosphorylation

This study shows that relaxin reduces estrogen receptor alpha phosphorylation and alters estrogen-dependent gene expression in myometrial cells.

RBN-2397, a PARP7 Inhibitor, Synergizes with Paclitaxel to Inhibit Proliferation and Migration of Ovarian Cancer Cells

This study shows that RBN-2397, a PARP7 inhibitor, reduces ovarian cancer cell proliferation and migration, and synergizes with paclitaxel to enhance these effects.

Development and Characterization of Recombinant ADP-Ribose Binding Reagents that Allow Simultaneous Detection of Mono and Poly ADP-Ribose

The paper develops and characterizes new recombinant ADP-ribose binding reagents that enable species-specific detection of mono and poly ADP-ribose in antibody-based assays.

KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription

The paper shows that KAP1 negatively regulates RNA polymerase II elongation kinetics to enhance signal-induced transcription of Immediate Early Genes.

Development and Characterization of PAR-Trackers: New Tools for Detecting Poly(ADP-ribose) In Vitro and In Vivo

The paper develops and characterizes PAR-Trackers, new tools for detecting poly(ADP-ribose) in vitro and in vivo, showing improved sensitivity and spatial-temporal resolution.

Natural Selection has Shaped Coding and Non-coding Transcription in Primate CD4+ T-cells

The paper compares nascent transcription in primates to identify evolutionary patterns in coding and non-coding transcription, finding that transcription factor binding sites and adaptive substitutions drive species-specific differences.

Accurate Identification of Active Transcriptional Regulatory Elements from Global Run-On and Sequencing Data

The paper introduces dREG, a machine-learning method to identify active transcriptional regulatory elements from GRO-seq data, showing strong enrichment for functional marks.