Otto Berninghausen

Dynamic architecture of the Rixosome reveals mechanism of activation and ITS2 processing

The paper presents cryo-EM structures of the Rixosome and pre-60S particles to elucidate the dynamic architecture and activation mechanism of the Rixosome for ITS2 processing.

Yeast Dhx29 promotes translation progression by unwinding structured mRNA in the ribosomal A-site

This study shows that yeast Dhx29 unwinds structured mRNA in the ribosomal A-site during translation elongation, resolving hairpin structures and preventing ribosome collisions.

Exopolysaccharide export complex PelBC of Pseudomonas aeruginosa attenuates the dynamics of the surrounding outer membrane

The paper examines the structure and function of the PelBC export complex in Pseudomonas aeruginosa, showing how it stabilizes outer membrane lipids and guides polysaccharide export.

Assembly and dynamics of the outer membrane exopolysaccharide transporter PelBC of Pseudomonas aeruginosa

This study determines the structure of the PelBC exopolysaccharide transporter in Pseudomonas aeruginosa and identifies its gating mechanism for exopolysaccharide transport.

Substrate-induced assembly and functional mechanism of the bacterial membrane protein insertase SecYEG-YidC

The paper visualizes the biogenesis of a bacterial membrane protein using cryo-EM and cell-free synthesis, revealing a gate-independent insertion mechanism involving SecYEG and YidC.

H/ACA snoRNP guides ribosomal RNA subdomain folding in a satellite particle before joining the core 90S pre-ribosome

The paper shows that the H/ACA snoRNP snR30 forms a satellite particle to guide 18S rRNA subdomain folding before integrating into the 90S pre-ribosome, enhancing ribosome assembly accuracy.

The RNA helicase HrpA rescues collided ribosomes in E. coli

The paper shows that HrpA, an RNA helicase in E. coli, rescues stalled ribosomes by splitting collided ribosomes into subunits using ATP hydrolysis.

B. subtilis MutS2 splits stalled ribosomesinto subunits without mRNA cleavage

This study shows that MutS2 in B. subtilis splits stalled ribosomes into subunits without cleaving mRNA, differing from the E. coli SmrB mechanism.

Recognition and deubiquitination of free 40S for translational reset by Otu2

The paper identifies Otu2's role in deubiquitinating eS7 on recycled 40S ribosomal subunits to reset translation, revealing structural mechanisms for this process.

Ribosome collisions in bacteria promote ribosome rescue by triggering mRNA cleavage by SmrB

The paper identifies SmrB as a factor that cleaves mRNAs upstream of stalled ribosomes in bacteria, promoting ribosome rescue through ribosome collisions.

Structural inventory of native ribosomal ABCE1-43S pre-initiation complexes

The paper uses cryo-EM to characterize native ABCE1-containing pre-initiation complexes in yeast and human cells, revealing their structural roles in translation initiation.

Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.

The paper presents cryo-EM structures of hibernating yeast and human ribosomes, showing Lso2/CCDC124 binds in a non-rotated state, enabling rapid translation reactivation.

Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2

This study reveals how SARS-CoV-2 Nsp1 protein inhibits host translation and immune responses by binding ribosomes and blocking mRNA entry.

The Ccr4-Not complex monitors the translating ribosome for codon optimality

The paper shows the Ccr4-Not complex interacts with the ribosome's E-site when codon optimality is low, linking translation efficiency to mRNA stability.

Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1

The paper presents cryo-EM structures of hibernating 70S and 100S ribosomes from E. coli, revealing the roles of HPF and RMF in ribosome inactivation and dimerization.