Carcinogens and Genotoxicity Assessment

Protocol for assessing DNA damage levels in vivo in rodent testicular germ cells in the Alkaline Comet Assay

This paper presents a protocol to measure DNA damage in rodent testicular germ cells using a modified alkaline comet assay, enabling assessment of genotoxic effects on spermatids and primary spermatocytes.

Investigating BPDE-induced embryonic toxicity employing hiPSC-based models

This study examines BPDE toxicity in hiPSCs and NPCs, revealing hiPSCs are more sensitive to BPDE with enhanced p53-mediated DNA damage response, and NBS patient cells show reduced apoptosis.

Dose-related Mutagenic and Clastogenic Effects of Benzofluoranthene in Mouse Somatic Tissues Detected by Duplex Sequencing and the Micronucleus Assay

This study assesses the mutagenic and clastogenic effects of benzo[b]fluoranthene in mice using Duplex Sequencing and the micronucleus assay, showing dose-dependent increases in mutation frequency and cancer-related mutation spectra.

N-Acetyl-2-Aminofluorene (AAF): In vivo temporal expression patterns of growth cycle-dependent macromolecular binding constants KD are revealed by primary cultures of premalignant hepatocytes derived from a multi-cyclic hepatocarcinogenic feeding regimen

The paper investigates how macromolecular binding constants of AAF change during hepatocarcinogenesis using primary cultures of premalignant hepatocytes.

Systematic analysis of gut bacterial carcinogen metabolism and its functional consequences

The paper systematically analyzes gut bacterial metabolism of carcinogens and shows bidirectional interactions with host metabolism, highlighting the microbiome's role in tumorigenesis.

High-Throughput Screening of the Saccharomyces cerevisiae Genome for 2-Amino-3-Methylimidazo Quinoline Resistance Identifies Colon Cancer-Associated Genes

The paper screens yeast for resistance to a carcinogen, identifies genes linked to colon cancer, and suggests potential mechanisms for HAA toxicity.

Environmental carcinogens disproportionally mutate genes implicated in neurodevelopmental disorders

The paper shows environmental carcinogens like PAHs disproportionately mutate genes linked to neurodevelopmental disorders such as ASD and schizophrenia.

Adopting Duplex Sequencing Technology for Genetic Toxicity Testing: A Proof-of-Concept Mutagenesis Experiment with N-Ethyl-N-Nitrosourea (ENU)-Exposed Rats

This paper evaluates a DuplexSeq-based mutagenesis assay in ENU-exposed rats, showing early detection of mutations and reproducible results across tissues and labs.

Duplex Sequencing Provides Detailed Characterization of Mutation Frequencies and Spectra in the Bone Marrow of MutaMouse Males Exposed to Procarbazine Hydrochloride

This study uses Duplex Sequencing to assess mutation frequencies and spectra in MutaMouse bone marrow after procarbazine exposure, showing its advantages over conventional assays.

Error-corrected Duplex Sequencing enables direct detection and quantification of mutations in human TK6 cells with remarkable inter-laboratory consistency

This study demonstrates that error-corrected Duplex Sequencing reliably detects and quantifies mutations in human TK6 cells across different laboratories and time points.

Development of a versatile high-throughput mutagenesis assay with multiplexed short read NGS using DNA-barcoded supF shuttle vector library amplified in non-SOS E. coli

The paper develops a high-throughput mutagenesis assay using DNA-barcoded supF shuttle vectors and NGS to study mutation frequencies and spectra in E. coli.

Analyses of Mutational Patterns Induced by Formaldehyde and Acetaldehyde Reveal Similarity to a Common Mutational Signature

This study analyzes mutational patterns induced by formaldehyde and acetaldehyde, revealing similarities to a known mutational signature and distinguishing features between the two compounds.

Artificial Intelligence uncovers carcinogenic human metabolites

The paper develops Metabokiller, an AI model that predicts carcinogenic human metabolites by analyzing their biochemical properties and validates predictions through experimental assays.

Acetaldehyde makes a distinct mutation signature in single-stranded DNA

This study shows acetaldehyde causes strand-biased G[->]T mutations on single-stranded DNA via Polζ bypass and identifies a unique mutation signature in alcohol-related cancers.

Statistical notes on false positive and negative error rates in the evaluation of long-term carcinogenicity bioassays

The paper examines false positive and negative error rates in long-term carcinogenicity bioassays, focusing on their dependence on analysis methods and dose-response design.

Robust statistical evaluation of tumor incidences in long-term rodent carcinogenicity studies: the reformulated poly-k trend test

The paper reformulates the poly-k trend test to evaluate tumor incidences in rodent carcinogenicity studies by considering multiple dose-response shapes and statistical models.

Genotoxicity evaluation of levonorgestrel-releasing intrauterine system (LNG-IUS) in exfoliated cervical cells using the micronucleus (MN) test

This study evaluates the genotoxicity of LNG-IUS by analyzing micronuclei and other nuclear anomalies in cervical cells before and after insertion, finding no significant increase in micronuclei but increased cytotoxicity-related changes.

Direct Quantification of in vivo Mutagenesis and Carcinogenesis Using Duplex Sequencing

This paper demonstrates that Duplex Sequencing, an error-corrected NGS method, can accurately quantify in vivo mutagenesis and early signs of carcinogenesis in mice, outperforming traditional transgenic rodent assays.

SEQUENCING CHEMICALLY INDUCED MUTATIONS IN THE MUTAMOUSE LACZ REPORTER GENE IDENTIFIES HUMAN CANCER MUTATIONAL SIGNATURES

The Carcinogenome Project: In-vitro Gene Expression Profiling of Chemical Perturbations to Predict Long-Term Carcinogenicity

The paper develops an in-vitro gene expression screening method to predict chemical carcinogenicity and genotoxicity using HepG2 cells and machine learning models.