Advanced Fluorescence Microscopy Techniques

The paper demonstrates prolonged single-neuron voltage imaging in behaving mice using ElectraOFF, revealing plasticity changes during neuromodulation.

The paper introduces Vega, a photostable GEVI with high sensitivity and fast response, enabling high-fidelity voltage imaging in tissue and live animals.

DeepTRACE is a machine learning tool that analyzes single-molecule tracks in living cells, uncovering molecular processes and relationships not present in training data.

The paper develops IntAct-U-ExM to visualize actin networks with super-resolution, showing improved resolution of actin structures in yeast and mammalian cells.

This paper introduces a spectral CSD-ISM system for high-resolution, multi-color imaging with improved speed and resolution.

This paper introduces SiLM, a chip-based 3D single-molecule localization microscopy method that achieves high axial precision with a single-objective lens, comparable to 4Pi methods.

The paper introduces four new fluorescent protein fusions for Dorsal to study its dynamics in fruit fly embryos.

The paper introduces CoSiDeX, a tool for selecting and decoding fluorescent protein palettes in multiplexed imaging experiments using hyperspectral data.

This paper introduces VLM, a super-resolution microscopy method that combines hardware and algorithmic improvements to achieve high-fidelity 3D single-molecule imaging with 10 nm and 25 nm localization precision.

This study uses FRAP-SR to reveal subcompartments within 53BP1 foci, showing differential protein mobility and function in different foci types.

The paper introduces GEQO biosensors for absolute analyte quantification in single cells without requiring specialized equipment, showing they account for buffering and retain time trace information.

This paper introduces a hierarchical adaptive optics strategy to improve three-photon imaging in the mouse brain during behavior by correcting optical aberrations at multiple levels.

This paper develops a framework for simultaneous particle tracking, phase retrieval, and PSF reconstruction to improve 3D tracking of lytic granules in T cells.

This paper introduces a mesoscopic SCAPE microscope with a super-oblique light sheet and rescanning strategy, achieving high-resolution volumetric imaging over large fields of view.

The paper develops high-performance green and red fluorescent biosensors for pyruvate with large fluorescence intensity changes and varying affinities, enabling multicolor imaging in mammalian cells.

The paper introduces ABDS, an inexpensive open-source dye for eukaryotic cell visualization, and provides its preparation method and usage examples.

The paper introduces AutoDS and AutoDS3D, deep learning tools for single-molecule super-resolution microscopy that reduce manual intervention and computation time.

Alpha-BET uses AlphaFold to identify optimal sites for labeling viral glycoproteins with nanobodies, enabling high-resolution imaging of viral structures without disrupting their function.

The paper improves single-molecule localization microscopy by extending temporal context using a hybrid U-Net and Transformer model, achieving better resolution than existing methods.

This paper provides a practical guide for Single-Particle Tracking in live-cell imaging, covering sample preparation, microscope settings, and analysis software comparison.

The paper improves a GABA sensor, iGABASnFR2, with higher sensitivity and faster kinetics, enabling better in vivo imaging of GABAergic transmission.

The paper introduces a LIFT microscope for three-photon imaging with a large field of view, enabling high-resolution calcium imaging in deep brain regions of mice and rats.

The paper introduces a mouse line for labeling blood and interstitial fluid with fluorescent proteins to study vascular structure and permeability in vivo.

This paper develops ATOM biosensors using mStayGold and mBaoJin to detect PSD95, Gephyrin, and HOMER1 with high specificity and turn-on ratios over 100-fold.

TranSIM is a high-speed volumetric imaging system that captures large-scale neural activity with reduced bandwidth constraints and improved spatial resolution.

This paper introduces SIMCODE, a method combining modulation patterns and deep learning to enhance single-molecule localization microscopy, achieving better resolution and performance in high-density samples.

This paper introduces 3D-FUDIP, a method that improves spatial resolution in fluorescence imaging by decoupling deblurring and denoising, achieving 8-fold resolution improvement and 6-fold SNR increase with reduced photon usage.

This paper introduces a new workflow for FLIM live-cell imaging using lifetime unmixing to distinguish fluorophores and demonstrates its application in tracking proteins and reducing autofluorescence in C. elegans.

This paper introduces IFCA, a method using independent component analysis to separate fluorescence signals from diffusing single molecules, demonstrating its effectiveness in analyzing complex molecular systems.

This paper introduces two multiplexed miniature two-photon microscopes (M-MINI2Ps) that enable high-speed neural imaging in freely moving mice with improved frame rates and spatial resolution.