Extracellular vesicles in disease

The paper develops a protocol combining Tangential Flow Filtration and Size Exclusion Chromatography to isolate high-purity extracellular vesicles from muscle tissue, separating Wnt7a-bound EVs from free Wnt7a.

The paper identifies a network involving DIP-2, SAX-2, PAD-1, and TAT-5 that regulates neuronal morphology by controlling extracellular vesicle release in C. elegans.

This paper introduces CAD-LB, a microfluidic technique for rapid EV biomarker assessment, validated on nanoparticles, purified EVs, and clinical plasma samples.

This study identifies exosomal hemoglobin transfer from non-neuronal cells to neurons under hypoxia as a mechanism to maintain mitochondrial homeostasis and protect against neurological injury.

The paper identifies small RNAs in plasma extracellular vesicles as biomarkers for premanifest changes in Huntington's disease, showing their association with cognitive performance and longitudinal progression.

This paper introduces a label-free method for measuring extracellular vesicle size and integrates it with fluorescence imaging using interferometric scattering.

This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.

This study shows that mouse serum-derived small extracellular vesicles can reduce inflammatory pain through opioid signaling and immunomodulation.

This study investigates the immunomodulatory roles of platelet-derived extracellular vesicles by comparing their functional effects and omics profiles across different activation methods.

This paper introduces a method to rapidly and efficiently isolate extracellular vesicles with high purity and recovery, enabling downstream analyses for cancer diagnostics.

SPIRFISH detects protein and RNA in single nanoparticles, revealing variability in structure and RNA packaging efficiency.

This study compares homotypic and heterotypic EV interactions on uptake in BECs and macrophages, finding membrane fusion dominates uptake in low-pinocytic cells.

The paper identifies SRRM2 as a surface-exposed cancer target and shows its utility in CAR-T cell therapy.

This study shows that 27-hydroxycholesterol increases EV secretion by impairing lysosomal function through mitochondrial ROS, and antioxidant treatment reverses this effect.

The paper shows that mechanical uterine distention enhances exopher extrusion from proteostressed C. elegans neurons, linking mechanical force to extracellular vesicle production and neurodegenerative disease mechanisms.

This paper introduces a rapid and high-yield method for isolating extracellular vesicles with high purity, demonstrating its effectiveness across multiple sources and applications.

This study profiles miRNA in extracellular vesicles secreted during the maturation of human embryonic stem cell-derived RPE cells to inform EV-based therapeutics.

This paper develops an engineered extracellular vesicles-based vaccine platform for combined mRNA and protein delivery, showing tumor regression and immune responses in preclinical models.

The paper identifies hepatocyte-derived extracellular vesicles (Hep-EVs) as critical regulators of liver regeneration, showing they promote hepatocyte proliferation through Cdk1 activity and offer therapeutic potential.

This study identifies the Rubicon-WIPI axis as a regulator of exosome biogenesis, showing its role in age-dependent exosome release and microRNA fate in mice.

This study characterizes extracellular vesicles (EVs) from cyanobacteria, showing they contain chlorophyll intermediates and may help manage their accumulation.

This paper develops HiA Zippersomes to enhance EV accumulation and retention in the infarcted myocardium, improving cardiac function and reducing infarct size.

This study identifies sEV-RNAs that distinguish T1a stage colorectal cancer and advanced adenoma from normal controls using liquid biopsy.

This study compares the therapeutic efficacy of mitochondria-containing extracellular vesicles from mouse and human brain endothelial cells in a mouse stroke model, finding that mouse-derived vesicles are more effective.

This paper introduces a method for isolating surface-engineered extracellular vesicles using TFF-SEC, showing improved purity and efficiency compared to conventional methods.

This study shows that pneumolysin-loaded host extracellular vesicles contribute to pneumococcal pathogenesis by inducing cytotoxicity and inflammation.

The paper develops DETECTOR-seq to analyze fragmented cell-free RNAs in plasma and extracellular vesicles, revealing distinct RNA distributions and potential biomarkers for cancer detection.

The paper develops a HaloTag-based system for quantitatively characterizing engineered proteins on extracellular vesicles and compares it to antibody labeling.

This study shows that mesenchymal stem cell-derived extracellular vesicles reduce hippocampal NLRP3 inflammasome activation and inflammation in adolescent mice after binge ethanol treatment.

This paper introduces a fast and sensitive size-exclusion chromatography method for plasma extracellular vesicle proteomic analysis, demonstrating its utility in identifying EV proteins related to physical activity.