Bacteriophages and microbial interactions

Endemism shapes viral ecology and evolution in globally distributed hydrothermal vent ecosystems

This study identifies viruses from hydrothermal vents and shows that while many are endemic, some share proteins related to core functions, suggesting geology influences viral ecology and host coevolution.

Characterization of a virulent bacteriophage consortium targeting Enterobacteriaceae from inflamed preterm gut mucosa

This study isolates and characterizes virulent bacteriophages targeting Enterobacteriaceae from preterm piglets with NEC-like inflammation, showing their potential for phage therapy.

Characterization and Genomic Analysis of vB_CoeS_P1, the First Vibrio hepatarius Phage, and Definition of the New Family Estovirdae

The paper characterizes a novel Vibrio hepatarius phage, vB_CoeS_P1, and proposes a new family, Estovirdae, based on genomic and phylogenetic analysis.

Generation of a recombinant mimivirus by direct transfection of a PCR amplicon

This study develops a method to generate a recombinant mimivirus by directly transfecting a PCR amplicon into host cells, bypassing the need for plasmid construction.

Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence

This study shows that phage Seregon reduces Xcc virulence and disease symptoms in Arabidopsis by suppressing JA responses and bacterial virulence factors without increasing bacterial resistance.

Unraveling Resistance and Thresholds in Phage Therapy Using Staphylococcus aureus In Vitro Models

This study models phage-S. aureus interactions in vitro to identify resistance thresholds and optimal dosing strategies for phage therapy.

Homology-Based Enzymatic Assembly of Modular T7 Phage Genome

This paper introduces a method to assemble synthetic T7 phage genomes in vitro without restriction enzymes, enabling modular DNA insertion.

Studies in vitro e in vivo of Phage Therapy Medical Products (PTMPs) targeting clinical strains of Klebsiella pneumoniae belonging to the clone ST512

This study develops and tests phage therapy medical products (PTMPs) against carbapenem-resistant Klebsiella pneumoniae ST512 strains, showing partial efficacy and the need for antibiotic combination.

Expansion of the genomic and functional diversity of global ocean giant viruses

The paper presents 230 new GV genomes and 398 partial genomes from nine ocean datasets, revealing new functions and a functional divide between GV orders.

Role of Phage-Antibiotic Combinations in Reducing ESBL-Producing and Carbapenem-Resistant Escherichia coli

This study isolates and characterizes two phages effective against ESBL-producing E. coli and CREC, showing enhanced lysis and survival rates when combined with antibiotics.

Intraspecific bacterial competition mediated by rapidly diversifying tailocin and prophage loci

This study shows that Xenorhabdus bacteria use phage-related genes to diversify their weaponry and defense systems, enabling competitive interactions with closely related strains.

Engineering bacteriophages through deep mining of metagenomic motifs

This paper introduces Meta-SIFT, a method to discover functional sequence motifs in metagenomes to engineer bacteriophage activity, demonstrating its utility in creating variants with novel host specificity.

Stable coexistence and transport of lytic phage infections with migrating bacterial hosts

The paper shows how spatial interactions enable stable coexistence and transport of virulent phages with migrating bacteria, using theory, simulation, and experiments across multiple systems.

Phollow: Visualizing Gut Bacteriophage Transmission within Microbial Communities and Living Animals

Phollow tracks phage replication and spread in zebrafish guts with single-virion resolution, revealing rapid uptake by intestinal cells and antibiotic-induced shifts in microbial communities.

Automated Bacteriophage Evolution Studies with the Aionostat

The paper introduces Aionostat, an automated device for high-throughput bacteriophage evolution studies, demonstrating its ability to accelerate and standardize phage adaptation and recombination experiments.

Expanding the chicken gut virome: Uncovering viral diversity, host interactions, and regional variations across the intestinal tract

The paper analyzes chicken gut metagenomic data to characterize the virome, identifying novel viral sequences and their interactions with bacterial hosts across intestinal regions.

Deciphering the RNA-based regulation mechanism of the phage-encoded AbiF system in Clostridioides difficile

The paper identifies a new AbiF-like system in C. difficile and shows its regulation by a non-coding RNA through RNA-protein interactions.

Weberviruses are gut-associated phages that infect Klebsiella spp.

The paper identifies and characterizes seven new lytic phages infecting Klebsiella, confirms their classification as Webervirus, and identifies a new genus, while highlighting their potential as therapeutic agents against multidrug-resistant strains.

Diversity, structure-function relationships and evolution of cell wall-binding domains of staphylococcal phage endolysins

This study analyzes the structure-function relationships and evolutionary origins of cell wall-binding domains in staphylococcal phage endolysins, finding that CBD specificity is not strongly correlated with phylogeny or host specificity.

Environmental differences impact Acinetobacter baumannii phage isolation and infectivity

This paper discovers a novel lytic phage, Mystique, that infects a broad range of Acinetobacter baumannii strains and shows that environmental conditions significantly affect phage infectivity and isolation outcomes.

The food-gut axis: the phyllosphere virome of fresh vegetables is diverse, abundant, and transmitted to the human gut virome

The study examines the virome of fresh vegetables and its transmission to the human gut, finding that vegetable viruses contribute to gut virome diversity.

Latent infection of an active giant endogenous virus in a unicellular green alga

The paper identifies an active giant endogenous virus in Chlamydomonas reinhardtii, showing viral production and latent infection dynamics in natural isolates.

Filamentous bacteriophages induce proinflammatory responses in intestinal epithelial cells

This study shows that filamentous bacteriophages like M13 induce proinflammatory responses and inhibit bacterial internalization in intestinal epithelial cells.

Mitomycin C eliminates cyanobacterial transcription without detectable lysogen induction in a Microcystis-dominated bloom in Lake Erie

The paper tests lysogeny in Microcystis blooms using mitomycin C and finds it ineffective for inducing prophage, suggesting standard protocols are unsuitable for freshwater cyanobacterial studies.

Extreme diversity of phage amplification rates and phage-antibiotic interactions revealed by PHORCE

This paper introduces PHORCE, a method to quantify phage amplification rates and reveals wide variation in phage-antibiotic interactions across Escherichia coli phages.

Improving gut virome comparisons using predicted phage host information

This paper introduces predicted phage host families to improve cross-study comparisons of gut viromes by reducing ecological distances and detecting disease-specific signatures.

Completing the BASEL phage collection to unlock hidden diversity for systematic exploration of phage-host interactions

The paper completes the BASEL phage collection by overcoming host limitations to isolate new phages, revealing insights into viral host recognition and bacterial immunity.

Genetic modification of intractable staphylococcal clones by heat-shock facilitated phage transduction

This paper introduces a method to genetically modify intractable staphylococcal clones using heat-shock facilitated phage transduction, enabling DNA introduction into bacteria with restriction barriers.

High-content assessment of Pseudomonas aeruginosa bacteriophage efficacy reveals host genetic factors involved in phage specificity

The paper develops a high-content screen to identify effective Pseudomonas aeruginosa phages and identifies host genes influencing phage specificity.

A phage-encoded counter-defense inhibits an NAD-degrading anti-phage defense system

The paper identifies a phage-encoded counter-defense gene that inhibits a bacterial NAD-degrading anti-phage defense system, enabling phage survival.