Advanced X-ray Imaging Techniques

Revealing multiscale architecture of the intact human spine using hierarchical phase contrast tomography

This paper uses HiP-CT to image the human spine at high resolution, revealing multiscale anatomy and microstructural features without exogenous contrast.

A method for correcting background non-uniformity while preserving specimen-level information in Fourier ptychographic microscopy images

This paper introduces a background correction method for Fourier ptychographic microscopy images that preserves specimen information and improves quantitative analysis.

In situ radiation damage tracking during scanning small-angle X-ray scattering

This paper tracks radiation damage in cells during scanning SAXS using a synchronized brightfield microscope.

Cross-scale Imaging of Live Botanical Specimens using Fourier Ptychographic Microscopy

This paper applies Fourier ptychographic microscopy to label-free imaging of large botanical specimens, achieving high-resolution imaging over a large field of view.

An imaging framework for nuclei-based three-dimensional cell quantification in intact tissue using phase-contrast X-ray CT

This paper develops an imaging framework using phase-contrast X-ray CT to quantify nuclei in intact unstained tissue, showing comparable results to histology.

Physics-aware resolution enhancement of soft X-ray tomography with measurement-supervised learning

This paper enhances the spatial resolution of soft X-ray tomography using physics-aware learning, improving structural fidelity and reducing radiation exposure.

Sampling Mismatch and Correction for Ptychographic Single-Particle Analysis

This study identifies sampling mismatch in ptychographic single-particle analysis and shows that correcting it improves resolution by ~1.5 Å.

Open Fourier Ptychographic Microscopy (OpenFPM)

OpenFPM provides an open-source, low-cost Fourier ptychographic microscopy platform for high-resolution biomedical imaging.

Synchrotron phase contrast micro-CT of prostate tissue

This study uses synchrotron phase contrast micro-CT to image prostate tissue, comparing its structural features with histology and MRI data.

Achieving Micrometer-Scale 4D X-ray tomography of Living Leaf Tissue in the Laboratory

This paper develops a method for micrometer-scale 4D X-ray tomography of living leaf tissue, demonstrating stable imaging over 22 hours with minimal radiation damage.

Histology-guided 3D virtual staining of microCT-imaged lung tissue via deep learning

This paper develops a deep learning method to virtually stain 3D microCT lung tissue, mimicking histological stains and enabling 3D pathology analysis.

Evolution of Hierarchical Phase-Contrast Tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study

This paper demonstrates the evolution of HiP-CT for whole adult human brain imaging, showing improvements in data quality, resolution, and speed through hardware and scanning technique advancements.

Label-free 3D virtual histology of human formalin-fixed paraffin-embedded (FFPE) prostate needle biopsies with propagation-based phase-contrast micro-CT (PBCT)

This paper introduces a method using PBCT to create 3D virtual histology of FFPE prostate biopsies, enabling nondestructive 3D analysis of tumor architecture and distinguishing Gleason patterns.

Correlative X-ray imaging and fluorescence microscopy

This paper presents a correlative imaging platform combining fluorescence microscopy and scanning SAXS to study cellular structures in native conditions, showing agreement between label-free SAXS and labeled fluorescence data.

Fast scanning small angle X-ray scattering of hydrated biological cells

This paper demonstrates fast scanning SAXS on biological cells in aqueous environments to reveal intracellular anisotropy and orientation and assess radiation damage.

Critical point drying of brain tissue for X-ray phase contrast imaging

This paper demonstrates that critical point drying enhances X-ray phase contrast in mouse brain tissue, improving imaging resolution and compatibility with further processing techniques.

The Human Organ Atlas

The Human Organ Atlas provides open access to multiscale 3D imaging of human organs, along with tools and resources for analysis and research.

Correlative Synchrotron X-ray Microscopy Reveals Dose- and Division-Dependent Nanoparticle Redistribution in Macrophages

This study uses synchrotron-based correlative X-ray microscopy to show how fluorescent silica nanoparticles redistribute in macrophages with dose and division, revealing perinuclear clustering and nuclear-envelope deformations.

Preserving Tissue Integrity Under the Beam: High-Energy, Low-Dose Synchrotron CT for in situ Imaging of Bovine Intervertebral Discs

This study shows that low-dose synchrotron CT can image bovine intervertebral discs repeatedly without damaging their mechanical, microstructural, or molecular properties.

High-Resolution 3D Histology of the Murine Kidney Using Synchrotron X-Ray Micro-CT

This paper introduces a synchrotron-based 3D histology method for murine kidneys, achieving high-resolution imaging and quantitative morphometric analysis.

Cryo-Correlative Light and X-ray microscopies: Expanding the Intracellular Chemical Map

This paper introduces a combined cryo-microscopy workflow that enables nanoscale elemental mapping and imaging of cellular structures using heavy element probes.

Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections

The paper describes a bending magnet beamline with capillary optics for X-ray fluorescence microscopy, comparing spatial resolution and fluence between two microscopy stations.

The Extremely Brilliant Brain: An Isotropic Microscale Human Brain Dataset

The paper presents a high-resolution isotropic human brain dataset and demonstrates its use for neuroanatomy analysis and visualization.

Hierarchical Phase-Contrast Tomography Imaging: Applicability in biomedical research

This paper demonstrates the application of Hierarchical Phase-Contrast Tomography (HiP-CT) in three biomedical case studies, revealing detailed organ structures at multiple scales.

Compact Tape-Driven Sample Delivery System for Serial Femtosecond Crystallography

The paper introduces a compact tape-driven system for serial femtosecond crystallography that delivers sample droplets and initiates reactions, enabling efficient data collection.

X-Ray contrast-adjustable 3D printing for multimodal fusion of microCT and histology

This paper introduces a method to create 3D-printed reference structures with X-ray contrast agents for precise multimodal fusion of microCT and histology.

Self-Supervised Missing Wedge Correction in Soft X-Ray Tomography: Towards Accurate Cellular Morphology and Volume Quantification

This paper introduces a self-supervised model to correct missing wedge artifacts in soft X-ray tomography, improving volume quantification and morphology accuracy in cellular structures.

Resolving near-micron scale features within a whole sheep head using Hierarchical Phase-Contrast Tomography

This paper demonstrates HiP-CT with EPR for high-resolution imaging of a whole sheep head, enabling near-micron scale visualization of anatomical features without sectioning.

Interior soft x-ray tomography with sparse global sampling

This paper develops a sparse reconstruction algorithm for soft X-ray tomography to achieve high spatial resolution and whole-cell imaging with reduced radiation dose.

Demonstrating Soft X-Ray Tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy

The paper demonstrates the SXT-100, a compact soft X-ray microscope, for correlative cryogenic biological imaging with high resolution and minimal sample damage.