Advanced X-ray Imaging Techniques

An imaging framework for nuclei-based three-dimensional cell quantification in intact tissue using phase-contrast X-ray CT

This paper develops an imaging framework using phase-contrast X-ray CT to quantify nuclei in intact unstained tissue, showing comparable results to histology.

Sampling Mismatch and Correction for Ptychographic Single-Particle Analysis

This study identifies sampling mismatch in ptychographic single-particle analysis and shows that correcting it improves resolution by ~1.5 Å.

Open Fourier Ptychographic Microscopy (OpenFPM)

OpenFPM provides an open-source, low-cost Fourier ptychographic microscopy platform for high-resolution biomedical imaging.

Achieving Micrometer-Scale 4D X-ray tomography of Living Leaf Tissue in the Laboratory

This paper develops a method for micrometer-scale 4D X-ray tomography of living leaf tissue, demonstrating stable imaging over 22 hours with minimal radiation damage.

Histology-guided 3D virtual staining of microCT-imaged lung tissue via deep learning

This paper develops a deep learning method to virtually stain 3D microCT lung tissue, mimicking histological stains and enabling 3D pathology analysis.

Evolution of Hierarchical Phase-Contrast Tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study

This paper demonstrates the evolution of HiP-CT for whole adult human brain imaging, showing improvements in data quality, resolution, and speed through hardware and scanning technique advancements.

Fast scanning small angle X-ray scattering of hydrated biological cells

This paper demonstrates fast scanning SAXS on biological cells in aqueous environments to reveal intracellular anisotropy and orientation and assess radiation damage.

Critical point drying of brain tissue for X-ray phase contrast imaging

This paper demonstrates that critical point drying enhances X-ray phase contrast in mouse brain tissue, improving imaging resolution and compatibility with further processing techniques.

The Human Organ Atlas

The Human Organ Atlas provides open access to multiscale 3D imaging of human organs, along with tools and resources for analysis and research.

Preserving Tissue Integrity Under the Beam: High-Energy, Low-Dose Synchrotron CT for in situ Imaging of Bovine Intervertebral Discs

This study shows that low-dose synchrotron CT can image bovine intervertebral discs repeatedly without damaging their mechanical, microstructural, or molecular properties.

Cryo-Correlative Light and X-ray microscopies: Expanding the Intracellular Chemical Map

This paper introduces a combined cryo-microscopy workflow that enables nanoscale elemental mapping and imaging of cellular structures using heavy element probes.

Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections

The paper describes a bending magnet beamline with capillary optics for X-ray fluorescence microscopy, comparing spatial resolution and fluence between two microscopy stations.

Compact Tape-Driven Sample Delivery System for Serial Femtosecond Crystallography

The paper introduces a compact tape-driven system for serial femtosecond crystallography that delivers sample droplets and initiates reactions, enabling efficient data collection.

X-Ray contrast-adjustable 3D printing for multimodal fusion of microCT and histology

This paper introduces a method to create 3D-printed reference structures with X-ray contrast agents for precise multimodal fusion of microCT and histology.

Interior soft x-ray tomography with sparse global sampling

This paper develops a sparse reconstruction algorithm for soft X-ray tomography to achieve high spatial resolution and whole-cell imaging with reduced radiation dose.

Demonstrating Soft X-Ray Tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy

The paper demonstrates the SXT-100, a compact soft X-ray microscope, for correlative cryogenic biological imaging with high resolution and minimal sample damage.

3D X-ray microscopy lights up nanoparticles in plants

This paper introduces a 3D X-ray microscopy method to visualize nanoparticle transport in living plant tissue with sub-cellular resolution.

Integrative Imaging of Lung Micro Structure: Amplifying Classical Histology by Paraffin Block μCT and same-slide Scanning Electron Microscopy

The paper develops an integrative imaging pipeline combining micro-CT and scanning electron microscopy with histology to enhance lung tissue analysis by adding 3D context and ultrastructural details.

Micro to macro scale anatomical analysis of the human hippocampal arteries with synchrotron Hierarchical Phase-Contrast Tomography

This study uses synchrotron-based imaging to visualize and quantify the arterial microvasculature of the human hippocampus at high resolution.

Visualisation of gene expression within the context of tissues: an X-ray computed tomography-based multimodal approach

This paper develops a multimodal approach combining X-ray computed tomography and light-sheet fluorescence microscopy to visualize gene expression in 3D tissue context, applicable to in vitro models and model organisms.

Protocol for 3D Virtual Histology of Unstained Human Brain Tissue using Synchrotron Radiation Phase-Contrast Microtomography

This paper provides a protocol for 3D imaging of unstained human brain tissue using synchrotron radiation phase-contrast microtomography, enabling detailed anatomical analysis at the cellular level.

Gene Expression Detection in Developing Mouse Tissue Using In Situ Hybridization and microCT Imaging

The paper introduces GECT, a method combining in situ hybridization with microCT to detect gene expression in developing mouse tissues, showing compatibility with existing imaging techniques.

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

The paper demonstrates the use of a femtosecond laser to prepare resin-embedded samples for correlative microscopy workflows, enabling precise targeting and trimming for high-resolution imaging techniques.

A Wide-Field Micro-Computed Tomography Detector: Micron Resolution at Half-centimeter Scale

This paper introduces a micro-CT detector with 0.5 µm pixel size and 6 mm field-of-view, achieving 1.182 µm spatial resolution for 3D imaging of micron-scale structures in biological and non-biological samples.

Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models

This paper introduces an X-ray phase-contrast tomography method to map myelin in whole rodent brains without staining, showing it can detect and quantify white-matter injuries in disease models.

Brain virtual histology with X-ray phase-contrast tomography Part II: 3D morphologies of amyloid-β plaques in Alzheimer's disease models

This paper introduces a method using X-ray phase-contrast tomography to analyze 3D morphology of amyloid-β plaques in Alzheimer's disease mouse models and identifies differences in plaque characteristics across strains.

FPM app: an open-source application for simple and intuitive Fourier ptychographic reconstruction

The paper introduces an open-source FPM app for intuitive and efficient Fourier ptychographic microscopy reconstruction.

Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants

This paper introduces a scalable method for micro-CT analysis to quantitatively characterize brain lesions and implants while preserving the skull.

A Simple Nuclear Contrast Staining Method for MicroCT-Based 3D Histology Using Lead(II) Acetate

The paper introduces a simple lead(II) acetate staining method for microCT-based 3D histology that enhances nuclear contrast without requiring traditional staining or drying.

Dense neuronal reconstruction through X-ray holographic nano-tomography

This paper introduces X-ray holographic nano-tomography for imaging dense neuronal structures in Drosophila and mammals, using convolutional neural networks for automated segmentation.