Xenotransplantation and immune response

Proteomic Identification of Pig Xenoantigens for Clinical Xenotransplantation

This study identifies pig xenoantigens reactive to human preformed antibodies using proteomic and glycoproteomic methods, revealing potential targets for improving xenotransplantation.

Coordinated circulating and tissue-based T cell responses precede xenograft rejection

This study tracks T cell clonotypes across tissues in a pig-to-human kidney xenotransplantation model, showing coordinated immune responses preceding xenograft rejection.

Morula complementation restores fetal kidneys in xenocompatible SALL1 null sheep

This paper demonstrates that morula complementation with donor cells restores fetal kidney development in SALL1-null sheep, establishing sheep as a viable model for in vivo organ generation.

Development of a BM7G((TKO/hCD46/hCD55/hTHBD/hEPCR) Donor Pig with Endogenous Promoter-Driven Transgenes for Xenotransplantation

This paper develops a seven-gene modified pig model with endogenous promoter-driven transgenes to improve xenograft survival in xenotransplantation.

Xenophagocytosis blockade enhances interspecies chimerism

This study identifies xenophagocytosis as an innate immune barrier that limits interspecies chimerism and shows that blocking it enhances donor cell engraftment in mouse embryos.

Generation of cloned sheep lacking galactose-α1,3-galactose and Nglycolylneuraminic acid antigens

This paper generates cloned sheep lacking two xenoantigens, Gal and Neu5Gc, by knocking out their respective genes, creating a model that better mimics human immune status for preclinical testing.

A Haplotype-resolved Telomere-to-Telomere Pig Genome

This paper presents a haplotype-resolved telomere-to-telomere pig genome, revealing new protein-coding loci and enabling xenograft cell state analysis and interspecies chimerism compatibility scoring.

Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche

This study creates a single-cell atlas of pig-to-monkey kidney xenotransplantation, identifying macrophage chimerism and an IFN-ε-driven immune niche that protects the graft.

Specific Pathogen Free Ten Gene-Edited Pig Donor for Xenotransplantation

This study generates 10-gene-edited pigs that reduce immune rejection and zoonotic risks for xenotransplantation, showing improved survival in non-human primate recipients.

Engineering sialylation in pigs: A promising strategy for overcoming xenograft rejection and revolutionizing organ transplantation.

This paper demonstrates that expressing human sialyltransferase in pigs reduces immune rejection by altering sialic acid linkages, improving xenotransplantation outcomes.

Production and functional verification of 8-gene (GGTA1, CMAH, β4GalNT2, hCD46, hCD55, hCD59, hTBM, hCD39)-edited donor pigs for xenotransplantation

The paper edits 8 genes in pigs to reduce immune rejection and tests their effectiveness in xenotransplantation to non-human primates.

The SRG RAT supports human cell xenotransplantation through enhanced tumor microenvironment interactions

This study shows that SRG rats support better human tumor growth and microenvironment interactions compared to NSG mice, despite similar immunodeficiency levels.

Production of four-gene (GTKO/hCD55/hTBM/hCD39)-edited donor pigs and kidney xenotransplantation

The paper edits four genes in donor pigs and transplants their kidneys into monkeys, showing reduced immune rejection and successful graft survival.

Trabeculations of the porcine and human cardiac ventricles are different in number but similar in total volume.

The paper compares trabecular structures in porcine and human cardiac ventricles, finding similar total volumes but differences in number and growth patterns.

Integrative Multi-omic Profiling of Two Human Decedents Receiving Pig Heart Xenografts Reveals Strong Perturbations in Early Immune-Cell and Cellular Metabolism Responses.

The paper analyzes multi-omic data from two pig heart xenografts in human decedents to identify immune and metabolic responses associated with rejection and injury.

Xenotransplantation of porcine progenitor cells in an epileptic California sea lion (Zalophus californianus)

This study describes xenotransplantation of porcine interneuron progenitor cells into the hippocampus of an epileptic California sea lion, resulting in seizure cessation and stabilized clinical measures.

Extensive Mammalian Germline Genome Engineering

The paper modifies pigs' genomes to create organs compatible with human transplantation by inactivating PERVs and engineering immune-related genes.

Selective germline genome edited pigs and their long immune tolerance in Non Human Primates

The paper creates a genetically modified pig with specific gene edits to enable immune tolerance in non-human primates and demonstrates long-term graft survival without immunosuppression.