Tardigrade Biology and Ecology

Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome

This paper describes how the asexual tardigrade Hypsibius exemplaris modifies meiosis to maintain genome heterozygosity, providing insights into its reproductive mechanism and genetic inheritance.

Cytoplasmic Abundant Heat-Soluble Proteins from Tardigrades Protect Synthetic Cells Under Stress

The paper shows CAHS12 proteins protect synthetic cells under stress by forming a network that maintains membrane integrity and preserves encapsulated components.

Tardigrade Dsup: Interactions with DNA and protection of cells from oxidative stress

This study uses MD simulations and FLIM-FRET to show Dsup binds DNA in living cells and enhances cell survival under oxidative stress in mammalian and yeast cells.

Set to its place: first DNA data on freshwater tardigrades of Sakhalin (Far East Russia) shed light on the phylogenetic position of Mixibius (Eutardigrada: Hypsibioidea)

The paper presents the first DNA data on freshwater tardigrades from Sakhalin, revealing the phylogenetic position of Mixibius and transferring two species to a new genus.

Drying parameters and aging modulate protective properties of vitrified trehalose

This study examines how drying conditions, storage duration, and vitrified properties affect the stability of DNA, RNA, and enzymes in trehalose systems, finding that enzyme protection correlates with initial glass transition temperature but deteriorates with aging.

Chromosomes remain individualized through interphase in embryos of the tardigrade Hypsibius exemplaris

This study examines chromosome and nuclear architecture in early embryos of the tardigrade Hypsibius exemplaris, finding that chromosomes remain individualized and housed in separate lamin-lined compartments throughout interphase.

High resolution live imaging of tardigrade response to anoxia

This study uses high-resolution live imaging to observe tardigrade cellular changes during anoxia, revealing morphological and compositional shifts in storage cells.

Pigment-dispersing factor neuropeptides act as multifunctional hormones and modulators in tardigrades

This study identifies three PDFs in tardigrades and shows they function as multifunctional hormones and neuromodulators involved in various physiological processes.

Single-animal, single-tube RNA extraction for comparison of relative transcript levels via qRT-PCR in the tardigrade Hypsibius exemplaris.

This paper introduces a single-tardigrade RNA extraction method for qRT-PCR to compare transcript levels in Hypsibius exemplaris.

Ecdysteroid-dependent molting in tardigrades

This study shows that ecdysteroid hormones regulate molting in tardigrades, supporting an ancestral role in ecdysozoans and identifying a neural center for molting regulation.

A bacterial expression cloning screen reveals tardigrade single-stranded DNA-binding proteins as potent desicco-protectants

The paper identifies tardigrade mitochondrial single-stranded DNA-binding proteins as effective desiccation protectants through a bacterial expression cloning screen.

Comparative study of gamma radiation tolerance between desiccation-sensitive and desiccation-tolerant tardigrades

This study compares gamma radiation tolerance between desiccation-sensitive and desiccation-tolerant tardigrades, finding that desiccation-sensitive species have lower radiation tolerance.

Comparative analysis of tardigrade locomotion across life stage, species, and pharmacological treatment

This study analyzes tardigrade locomotion across life stages, species, and pharmacological treatments, revealing independent control of high-level walking parameters from leg coordination circuits.

Single-step generation of homozygous knock-out/knock-in individuals in an extremotolerant parthenogenetic tardigrade using DIPA-CRISPR

This study develops a method to generate homozygous gene-edited tardigrades using DIPA-CRISPR, enabling in vivo analysis of extreme stress tolerance mechanisms.

Tardigrade secretory proteins protect biological structures from desiccation

This study investigates SAHS proteins from tardigrades, showing they protect biological structures from desiccation by preventing liposome fusion and enhancing microbial survival.

Storage cell proliferation during somatic growth establishes that tardigrades are not eutelic organisms

This study shows that tardigrades undergo storage cell proliferation during growth, challenging the eutelic hypothesis and linking DNA replication to molting and survival.

Comparative transcriptomics reveal a novel tardigrade specific DNA binding protein induced in response to ionizing radiation

The paper identifies a novel tardigrade-specific DNA binding protein, TDR1, that enhances resistance to ionizing radiation in human cells.

Organisation of the nervous system in cysts of the freshwater tardigrade Thulinius ruffoi (Parachela, Isohypsibioidea: Doryphoribiidae)

This study examines the nervous system organisation and morphological changes in encysted Thulinius ruffoi tardigrades over 11 months.

The Embryonic Origin of Primordial Germ Cells in the Tardigrade Hypsibius exemplaris

This study identifies the embryonic origin of primordial germ cells in the tardigrade Hypsibius exemplaris using molecular markers and lineage tracing.

in vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada

The paper develops a genetic tool for tardigrades to study anhydrobiosis by enabling live imaging of protein dynamics.

LEA motifs promote desiccation tolerance in vivo

This study investigates the role of LEA-1 protein in desiccation tolerance in C. elegans, showing its protective effects against multiple stresses and identifying functional motifs that can be applied across species.

Cas9-mediated Genome Editing Reveals a Significant Contribution of Calcium Signaling Pathways to Anhydrobiosis in Pv11

The paper uses genome editing to show that calcium signaling pathways, specifically calmodulin-calcineurin-NFAT and calmodulin-calmodulin kinase-CREB, play distinct roles in anhydrobiosis in Pv11 cells.

The tardigrade Hypsibius exemplaris as an emerging model to study the mitochondrial alternative oxidase at animal organismal level

This study uses the tardigrade Hypsibius exemplaris to investigate mitochondrial alternative oxidase (AOX) activity and its role in animal physiology.

Dehydrated Caenorhabditis elegans stocks are resistant to multiple freeze-thaw cycles

This study shows that dehydrated C. elegans stocks can survive multiple freeze-thaw cycles using trehalose or glycerol.

Transcriptome analysis of Pv11 cells infers the mechanism of desiccation tolerance and recovery

This study uses CAGE-seq to analyze transcriptome changes in Pv11 cells during desiccation and rehydration, identifying DEGs and suggesting DNA repair as a recovery mechanism.

Comparative genomics of the tardigrades Hypsibius dujardini and Ramazzottius varieornatus

The paper compares the genomes of two tardigrade species to investigate their evolutionary relationships and mechanisms of extreme survival.