A. Lewis

A first insight into the developability of an IgG3: A combined computational and experimental approach

This study characterizes the developability of IgG3 mAbs using computational and experimental methods, finding poorer stability and higher viscosity compared to IgG1.

Single-genome analysis reveals heterogeneous association of the Herpes Simplex Virus genome with H3K27me2 and the reader PHF20L1 following infection of human fibroblasts

This study investigates the association of HSV-1 genomes with H3K27me2 and PHF20L1 in fibroblasts, finding that H3K27me2 is linked to genome silencing and that this association is influenced by demethylase activity and transcriptional state.

BMP antagonist CHRDL2 enhances the cancer stem cell phenotype and increases chemotherapy resistance in Colorectal Cancer

This study shows that CHRDL2 overexpression in CRC cells enhances chemotherapy resistance and stem-cell properties by boosting DNA repair and WNT signaling.

Oncogenic Ras activation in permissive somatic cells triggers rapid onset phenotypic plasticity and elicits a tumour-promoting neutrophil response

This study shows that oncogenic Ras in zebrafish skin triggers phenotypic plasticity in specific keratinocytes and promotes tumour-promoting neutrophils within 24 hours.

Candida spp. suppress neutrophil reactive nitrogen species to evade killing

This study shows that Candida spp. suppress neutrophil reactive nitrogen species to evade killing, and restoring RNS via Hif-1 stabilisation improves infection outcomes.

Tribbles1 and Cop1 cooperate to protect the host during in vivo mycobacterial infection

This study identifies Trib1 and Cop1 as immune regulators that protect against mycobacterial infection by modulating macrophage function and inflammation.

Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits

This study shows that rigidifying the TELSAM-target protein linker and removing the SUMO tag improves crystal diffraction resolution.

Small Molecule Modulation of the Archetypal UbiB protein COQ8

The paper develops small molecule inhibitors and activators for COQ8A, a UbiB family protein, using crystallography, NMR, and mass spectrometry to modulate its function in CoQ biosynthesis.

An arginase 2 promoter transgenic illuminates anti-inflammatory signalling in zebrafish

This study develops a zebrafish transgenic model to investigate anti-inflammatory signaling, showing arginase 2 is expressed in neutrophils during immune challenges.

Ion-dependent structure, dynamics, and allosteric coupling in a non-selective cation channel

This study investigates how bound Na+ and K+ ions affect the structure and dynamics of the NaK channel's selectivity filter, revealing dynamic behavior and allosteric coupling.

Hif-1alpha stabilisation is protective against infection in a zebrafish model of co-morbidity

This study shows Hif-1alpha stabilisation protects against tuberculosis infection in zebrafish even when inflammation is present.

A metagene of NRF2 expression is a prognostic biomarker in all stage colorectal cancer

The paper defines an NRF2 metagene and shows it predicts poor prognosis in all stages of colorectal cancer, with higher expression in CMS4 subtype.

Hif-1α stabilisation polarises macrophages via cyclooxygenase/prostaglandin E2 in vivo

This study shows hypoxia stabilizes Hif-1α, polarizing macrophages via COX/PGE2 to an M1 phenotype in zebrafish and human cells.

Glycan cross-feeding drives mutualism between Fusobacterium and the vaginal microbiota

This study shows that sialidase activity enables Fusobacterium to exploit sialoglycans, promoting its colonization and mutualistic interactions with the vaginal microbiota, which contributes to dysbiosis.

Hif-1alpha induced expression of Il-1beta protects against mycobacterial infection in zebrafish

The paper shows that Hif-1alpha increases Il-1beta expression in zebrafish macrophages, enhancing nitric oxide production and protecting against Mycobacterium marinum infection.