A. Pinto-Fernandez

USP16 is an ISG15 cross-reactive deubiquitinase targeting a subset of metabolic pathway-related proteins

This study identifies USP16 as an ISG15 cross-reactive deubiquitinase that regulates metabolic pathway-related proteins during type I interferon responses.

Human USP18 protects diverse cancer lineages from Type I Interferon independently of its canonical catalytic function

This paper shows USP18's scaffold function, not its catalytic activity, is critical for inhibiting cancer growth and Type I interferon signaling in human cells.

Sex-specific deubiquitylation drives immune-related neurodegeneration in Drosophila

This study shows that sex-specific deubiquitylation by Trabid in Drosophila influences neurodegeneration linked to immune responses and protein accumulation.

Structural Premise of Selective Deubiquitinase USP30 Inhibition by Small-Molecule Benzosulfonamides

This study investigates how a benzosulfonamide compound inhibits USP30, a mitochondrial deubiquitylating enzyme, using biochemical and structural methods to reveal its binding mechanism and selectivity.

Structural basis for antagonism of the ubiquitin ligase BIRC6 by SMAC

This study reveals how SMAC antagonizes BIRC6 by binding multiple sites on BIRC6, displacing caspases and inhibiting its ubiquitin ligase activity.

USP18 is an essential regulator of muscle cell differentiation and maturation

This study identifies USP18 as a critical regulator of muscle cell differentiation and maturation, independent of its role in the ISG response.

ABPP-HT* - Deep meets fast for activity-based profiling of deubiquitylating enzymes using advanced DIA mass spectrometry methods

The paper introduces ABPP-HT*, an improved ABPP method combining high depth and throughput for profiling deubiquitylating enzymes using DIA MS, achieving consistent profiling of 35-40 DUBs with reduced missing values.

Ubiquitinome analysis of articular cartilage post mechanical injury reveals a differential ubiquitination pattern of a subset of DUBs and proteins linked to the ERAD cellular response

The paper identifies a unique ubiquitin signature in injured articular cartilage, linking ERAD pathway activation and DUB activity to tissue injury and osteoarthritis.

ABPP-HT - high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context

This paper introduces ABPP-HT, a high-throughput method for assessing DUB inhibitor selectivity in cells, showing improved efficiency over traditional ABPP.

USP28 deletion and small molecule inhibition destabilises c-Myc and elicits regression of squamous cell lung carcinoma

This study shows that deleting or inhibiting USP28 reduces c-Myc levels and causes regression of lung squamous cell carcinoma in mice and human xenografts.

A novel USP30 inhibitor recapitulates genetic loss of USP30 and sets the trigger for PINK1-PARKIN amplification of mitochondrial ubiquitylation.

The paper identifies a USP30 inhibitor that mimics genetic loss of USP30, revealing its role in regulating mitochondrial ubiquitylation and mitophagy through TOM complex components.

The deubiquitylase USP9X controls ribosomal stalling

This study identifies USP9X as a deubiquitylase that regulates ZNF598 stability, impacting ribosomal quality control through modulation of di-ribosome resolution.

Deep analysis of the USP18-dependent ISGylome and proteome unveils important roles for USP18 in tumour cell antigenicity and radiosensitivity

This study identifies novel ISGylation targets regulated by USP18, showing its role in enhancing tumour cell antigenicity and reducing radiosensitivity in CML cells.