B. M. Kessler

SCF cyclin F - EXO1 axis controls cell cycle dependent execution of double strand break repair

The paper identifies SCF cyclin F's role in regulating EXO1 degradation to control cell cycle-dependent DSB repair pathways.

Proximity proteomics reveals UCH-L1 as an NLRP3 interactor that modulates IL-1β production in human macrophages and microglia

The paper identifies UCH-L1 as an NLRP3 interactor that modulates IL-1β production in human macrophages and microglia through proximity proteomics and functional assays.

USP16 is an ISG15 cross-reactive deubiquitinase targeting a subset of metabolic pathway-related proteins

This study identifies USP16 as an ISG15 cross-reactive deubiquitinase that regulates metabolic pathway-related proteins during type I interferon responses.

Novel protein interaction network of human calcitonin receptor-like receptor revealed by label-free quantitative proteomics

The paper identifies 46 novel proteins interacting with CLR in primary human dermal lymphatic endothelial cells using immunoprecipitation and mass spectrometry, confirmed by proximity ligation assay.

Human USP18 protects diverse cancer lineages from Type I Interferon independently of its canonical catalytic function

This paper shows USP18's scaffold function, not its catalytic activity, is critical for inhibiting cancer growth and Type I interferon signaling in human cells.

Sex-specific deubiquitylation drives immune-related neurodegeneration in Drosophila

This study shows that sex-specific deubiquitylation by Trabid in Drosophila influences neurodegeneration linked to immune responses and protein accumulation.

Structural Premise of Selective Deubiquitinase USP30 Inhibition by Small-Molecule Benzosulfonamides

This study investigates how a benzosulfonamide compound inhibits USP30, a mitochondrial deubiquitylating enzyme, using biochemical and structural methods to reveal its binding mechanism and selectivity.

Structural basis for antagonism of the ubiquitin ligase BIRC6 by SMAC

This study reveals how SMAC antagonizes BIRC6 by binding multiple sites on BIRC6, displacing caspases and inhibiting its ubiquitin ligase activity.

USP18 is an essential regulator of muscle cell differentiation and maturation

This study identifies USP18 as a critical regulator of muscle cell differentiation and maturation, independent of its role in the ISG response.

Targeted delivery of galunisertib attenuates fibrogenesis in an integrated ex vivo renal transplant and fibrosis model

Galunisertib supplementation during normothermic machine perfusion reduces fibrogenesis without harming renal function.

Deep topographic proteomics of a human brain tumour

This paper develops a workflow for spatially-resolved proteomics in human brain tumours, identifying spatial markers and immune-related protein networks in tissue slices.

ABPP-HT* - Deep meets fast for activity-based profiling of deubiquitylating enzymes using advanced DIA mass spectrometry methods

The paper introduces ABPP-HT*, an improved ABPP method combining high depth and throughput for profiling deubiquitylating enzymes using DIA MS, achieving consistent profiling of 35-40 DUBs with reduced missing values.

Ubiquitinome analysis of articular cartilage post mechanical injury reveals a differential ubiquitination pattern of a subset of DUBs and proteins linked to the ERAD cellular response

The paper identifies a unique ubiquitin signature in injured articular cartilage, linking ERAD pathway activation and DUB activity to tissue injury and osteoarthritis.

Integrative Omics Reveals Subtle Molecular Perturbations Following Ischemic Conditioning in a Porcine Kidney Transplant Model

This study uses integrative omics to detect subtle molecular changes in porcine kidneys after remote ischemic conditioning, finding reduced inflammation and potential fibrosis markers.

ABPP-HT - high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context

This paper introduces ABPP-HT, a high-throughput method for assessing DUB inhibitor selectivity in cells, showing improved efficiency over traditional ABPP.

USP28 deletion and small molecule inhibition destabilises c-Myc and elicits regression of squamous cell lung carcinoma

This study shows that deleting or inhibiting USP28 reduces c-Myc levels and causes regression of lung squamous cell carcinoma in mice and human xenografts.

The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function

This study shows that the chaperonin CCT8 is essential for T cell maturation, selection, and function by maintaining proteostasis, and its absence impairs immune protection against helminths.

A novel USP30 inhibitor recapitulates genetic loss of USP30 and sets the trigger for PINK1-PARKIN amplification of mitochondrial ubiquitylation.

The paper identifies a USP30 inhibitor that mimics genetic loss of USP30, revealing its role in regulating mitochondrial ubiquitylation and mitophagy through TOM complex components.

Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.

This study examines T cell immunity in UK COVID-19 patients, finding stronger and broader memory T cell responses in severe cases, with implications for vaccine design.

PYK2 controls intestinal inflammation via activation of IRF5 in macrophages

This study identifies PYK2 as a key regulator of intestinal inflammation by activating IRF5 in macrophages, showing reduced inflammation in colitis models and human ulcerative colitis biopsies.

The deubiquitylase USP9X controls ribosomal stalling

This study identifies USP9X as a deubiquitylase that regulates ZNF598 stability, impacting ribosomal quality control through modulation of di-ribosome resolution.

Deep analysis of the USP18-dependent ISGylome and proteome unveils important roles for USP18 in tumour cell antigenicity and radiosensitivity

This study identifies novel ISGylation targets regulated by USP18, showing its role in enhancing tumour cell antigenicity and reducing radiosensitivity in CML cells.

Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling

The paper maps interactions of ER ubiquitin ligases and identifies a complex involving RNF26 that modulates innate immune signaling via the cGAS-STING pathway.

Diagnostic Cerebrospinal Fluid Biomarker Discovery and Validation in Patients with Central Nervous System Infections

This study identifies and validates lipocalin 2 (LCN2) as a sensitive and specific biomarker for bacterial meningitis in cerebrospinal fluid.

BMP-signalling inhibition in Drosophila secondary cells remodels the seminal proteome, and self and rival ejaculate functions

This study shows that inhibiting BMP-signalling in Drosophila secondary cells alters seminal proteome composition and ejaculate function, revealing cross-talk between secretory cell types.

Ejaculate deterioration with male age, and its amelioration in Drosophila

The paper shows ejaculate performance declines with male age in Drosophila due to sperm and seminal protein deterioration, but this decline can be ameliorated by targeting insulin-like peptide-producing cells.

Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T-cells

The paper identifies synaptic ectosomes released by helper T cells that contain TCR and CD40L, enabling dendritic cell maturation and cytokine production.

Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

This study identifies ataxin-3 as a mediator of NOD2 and TLR2 signaling in myeloid cells, linking innate immune sensing to metabolic processes and mitochondrial function.