B. A. Schulman

Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST

This study uses nMOST to profile proteo-lipidomics in lysosomal storage disease mutants, revealing autophagy defects and mitochondrial dysfunction.

Visualizing chaperone-mediated multistep assembly of the human 20S proteasome

The paper uses cryo-EM to visualize the assembly of the human 20S proteasome, revealing how chaperones and propeptides guide subunit incorporation and proteasome activation.

Combinatorial selective ER-phagy remodels the ER during neurogenesis

The paper quantifies ER proteome remodeling during neurogenesis using ER-phagy receptors and identifies receptor-specific ER protein clearance mechanisms.

Structural snapshots along K48-linked ubiquitin chain formation by the HECT E3 UBR5

This study uses cryo-EM to reveal the structural mechanism of K48-linked ubiquitin chain formation by the HECT E3 ligase UBR5, showing how it orchestrates ubiquitin transfer through conformational changes.

Insights into the ISG15 transfer cascade by the UBE1L activating enzyme

This study presents a cryo-EM structure of the UBE1L-UBE2L6 complex bound to ISG15, revealing mechanisms of ISG15 recognition and conjugation pathway specificity.

Activity-based profiling of cullin-RING ligase networks by conformation-specific probes

The paper develops conformation-specific probes to profile activated cullin-RING ligase networks, revealing differential regulation across cell types and activation pathways.

PARK15/FBXO7 is dispensable for PINK1/Parkin-dependent mitophagy in iNeuron and HeLa cell systems

This study shows FBXO7 is not required for PINK1/Parkin-dependent mitophagy in HeLa and iNeuron cells, as its absence does not impair key mitophagy processes.

Multifaceted N-degron recognition and ubiquitylation by GID/CTLH E3 ligases

The paper identifies novel N-terminal motifs recognized by Gid4 and Gid10 E3 ligases and shows how structural flexibility and substrate context influence ubiquitylation efficiency.

GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme

The paper reveals a supramolecular chelate assembly mechanism by which E3 ligases target oligomeric substrates, demonstrating this in yeast and human orthologs.

A selective autophagy pathway for phase separated endocytic protein deposits

The paper identifies Ede1 as a selective autophagy receptor for endocytic protein condensates, showing its role in phase separation and autophagic degradation.

A Protein Engineering Approach for Uncovering Cryptic Ubiquitin-binding Sites: from a Ubiquitin-Variant Inhibitor of APC/C to K48 Chain Binding

The paper identifies a cryptic ubiquitin-binding site on APC/C using ubiquitin variants, revealing its preference for K48-linked chains and demonstrating a method for uncovering such sites in large complexes.