Keitaro Yamashita

Coordinated RNA- and protein-templated synthesis ofdouble-stranded DNA by a dual reverse transcriptase immune system

The paper shows how two reverse transcriptases with different templating strategies work together to produce complementary DNA for antiviral defense.

Structural mechanism of the type IX Retron-Kva2 anti-phage defense system

This study reveals the structural mechanism of the type IX Retron-Kva2 anti-phage defense system in Klebsiella variicola, showing how it cleaves host RNAs to inhibit phage infection.

Atomic resolution cryo-EM at 200 keV

The paper demonstrates atomic resolution cryo-EM at 200 kV using upgraded hardware, achieving 1.24 Å resolution comparable to 300 kV systems.

Measurement of atomic scattering factors by cryo-electron microscopy

This paper introduces a Bayesian method to estimate atomic scattering factors from cryo-EM data, improving accuracy by incorporating molecular environment effects.

Insights from aquaporin structures into drug-resistant sleeping sickness

This study determines the structure of TbAQP2 bound to drugs and glycerol, revealing how mutations in the channel lead to drug resistance in Trypanosoma brucei.

Neutron crystallographic refinement with REFMAC5 of the CCP4 suite

This paper extends the REFMAC5 program in the CCP4 suite to refine macromolecular models using neutron crystallographic data, improving the accuracy of hydrogen atom localization.

Abundant Aβ fibrils in ultracentrifugal supernatants of aqueous extracts from Alzheimer's disease brains

The paper reports the presence of abundant Aβ fibrils in aqueous extracts from Alzheimer's disease brains, with structures identical to those in amyloid plaques.

Structural insights into human excitatory amino acid transporter EAAT2

This study reports cryo-EM structures of human EAAT2 in substrate-free and inhibitor-bound states, revealing structural features and the mechanism of selective inhibition.

Cryo-EM structures of calcium homeostasis modulator channels in diverse oligomeric assemblies

The paper presents cryo-EM structures of CALHM channels from different species, revealing their ATP conduction and oligomerization mechanisms.

X-ray structures of human ETB receptor provide mechanistic insight into receptor activation and partial activation

The paper reports X-ray structures of the human ETB receptor bound to agonists, revealing molecular mechanisms of activation and partial agonism.