Kathy K. Niakan

The paper uses cryo-ET to reveal that cytoplasmic lattices in mouse embryos are ubiquitin-charged E2-E3 ligase assemblies involved in protein modification during development.

This study shows basement membrane mechanics regulate the spatial response to BMP4 in hPSCs and mouse embryos, disrupting gastrulation patterning when mechanics are altered.

This study identifies VGLL1 as a key regulator of trophoblast development by integrating BMP, WNT, and HIPPO signaling through chromatin remodeling and transcriptional control in human stem cells.

This study shows that XIST is required for XCI in human extraembryonic cells, which is essential for their survival and development.

This study identifies cell-type-specific DNA methylation dynamics in the human cortex across development, highlighting prenatal periods as critical for epigenomic plasticity and linking changes to neurodevelopmental disorders.

The paper shows that inhibiting ERK signaling expands epiblast cells capable of becoming naive pluripotent stem cells in humans, while FGF stimulation expands hypoblast markers.

This study generates the first single-cell transcriptomic atlas of marsupial gastrulation and early organogenesis, revealing accelerated transcriptional programs and novel tissues associated with developmental heterochrony.

This study shows that ETV4 and ETV5 regulate lineage specification and embryo patterning in mice by affecting primitive endoderm formation and pluripotency exit.

The paper investigates PADI6's role in oocyte cytoplasmic lattices using single-cell proteomics and transcriptomics, revealing its critical scaffolding function and connections to other oocyte compartments.

The paper examines sex differences in brain structure at birth in newborns, finding larger brain volumes in males and specific regional differences in gray and white matter volumes between sexes.

This study uses live imaging to show mitotic errors and lineage plasticity in human embryos before implantation, revealing implications for aneuploidy mosaicism.

The paper identifies transcription factors that can convert human embryonic stem cells into trophoblast stem cells, revealing their role in early placental development.

The paper shows Smad4 is not required before implantation but is essential for epiblast morphogenesis after implantation in mice.

The paper evaluates gene regulatory network prediction methods in early human embryos, finding that MICA, combining mutual information with chromatin accessibility, provides more reproducible and biologically meaningful results.

This study shows that human blastoids and pluripotent stem cells can enter a dormant state via mTOR inhibition, with distinct temporal dynamics compared to mice.

The paper investigates the role of YAP1 and TEAD1 in lineage segregation during human pre-implantation embryos, showing their involvement in trophectoderm and primitive endoderm formation independent of polarity.

The paper investigates the conservation of Hippo signaling in the first lineage specification event across four mammalian species, finding that inhibiting the Hippo pathway drives ectopic trophectoderm initiation in all species.

GIANI is an open-source software for automated analysis of 3D microscopy images, enabling segmentation, morphology quantification, and protein expression analysis in various imaging contexts.

The paper shows KLF17 can induce a naive-like transcriptome in primed hESCs but is not required for naive pluripotency establishment in vitro.

The paper analyzes single-cell genomics and transcriptomics data from CRISPR-Cas9-edited human embryos to assess unintended editing outcomes and loss-of-heterozygosity.