experimental assay

Parallel descending neurons differentially encode target and looming information in the hoverfly visual system

The paper identifies differences in how two types of descending neurons in hoverflies encode visual information from small targets, revealing distinct pathways for target pursuit and threat detection.

Optimal compositions of potent sliced-siRNA with DNA bases substitution and insights of Ago2 slicing actions

The paper investigates sliDRNAs, DNA nucleotide-substituted sliced-siRNAs, to understand their processing, maturation, and silencing mechanisms compared to siRNA and insights into Ago2 slicing actions.

Robust T and B cell activation is associated with rapid clearance of infectious SARS-CoV-2

The paper identifies immune cell activation patterns associated with rapid clearance of infectious SARS-CoV-2 in both unvaccinated and vaccinated individuals.

Targeting NF-κB prevents Schwann cell reprogramming and plexiform neurofibroma initiation in mouse model of Nf1

Compartment-Resolved Matrisome Shifts During Postnatal Tendon Maturation in Mice

This study uses compartment-resolved proteomics to analyze changes in the tendon matrisome during postnatal maturation in mice, revealing distinct trajectories in soluble and insoluble ECM components.

RNA-triggered Cas12a2 antibacterial activity and resistance plasmid degradation

This study demonstrates that an inducible CRISPR/Cas12a2 system can reduce bacterial growth and plasmid DNA in vitro and in vivo by targeting resistance transcripts.

Lipidic α-synuclein fibrils catalyze the formation of pathological mature fibrils

This study shows lipidic α-synuclein fibrils catalyze the formation of mature fibrils through a heterogeneous nucleation mechanism, lowering the energy barrier and following Ostwald's Rule of Stages.

Human lymphatic organoids reconstruct convergent developmental routes to functional lymphatic vessels

This study generates human lymphatic organoids from pluripotent stem cells using two mesodermal routes, showing they form functional vascular networks and support immune interactions.

Clonally Convergent Tph/Tfh-like CD4+ T Cells are Shared Across Recovery and Recurrence in Experimental Periodontitis

The paper identifies clonally convergent Tfh/Tph-like CD4+ T cells that persist in the mucosa after recovery from periodontitis and contribute to recurrence.

A hawkmoth's point of view - Innate symmetry preferences are informed by floral display and foraging function in an insect pollinator

This study shows that hawkmoths have innate symmetry preferences influenced by floral display planes, which enhance foraging success.

A Staphylococcus phage recombination cassette that enables recombineering across distant bacteria

The paper identifies a phage recombination cassette that enables recombineering across multiple bacterial species, including S. aureus, S. pyogenes, E. faecium, F. novicida, and E. coli.

Platycodin D2 Targets Vimentin to Suppress Fibroblast-Myofibroblast Transition: A Novel Therapeutic Strategy Against Pulmonary Fibrosis

This study identifies Platycodin D2 as a vimentin ligand that suppresses fibroblast-myofibroblast transition, offering a new therapeutic approach for pulmonary fibrosis.

Title: Combinatorial bnAb and T Cell Immunization Enhances Post-Infection Control of SHIVSF162p3 Viremia in Rhesus Macaques

This study shows that combining broadly neutralizing antibodies with T cell immunization improves post-infection control of SHIVSF162p3 viremia in rhesus macaques.

MAST1 is neuron-specific regulator of mitochondrial trafficking and abundance in human brain tissue

This study identifies MAST1 as a neuron-specific regulator of mitochondrial trafficking and abundance, linking its mutations to neuronal mitochondrial dysfunction and microcephaly.

BCL10 Gain-of-Function Impairs Germinal Center Participation by Naive B Cells and Cooperates with BCL6 to Drive Extrafollicular Diffuse Large B-Cell Lymphoma

This study shows BCL10 gain-of-function mutations prevent naive B cells from entering germinal centers and cooperate with BCL6 to cause extrafollicular diffuse large B-cell lymphoma.

The Pseudomonas protegens PBL3 secretome exerts antimicrobial activity against Burkholderia glumae by disrupting cell envelope homeostasis and impairing bacterial replication

Genetic Variation in Photosynthetic Maintenance and Post-Chill Recovery for Early Season Maize Plantings

This study evaluates genetic variation in maize's response to chilling stress and recovery, identifying heritable traits linked to cold resilience.

Hidden infections: Linking parasite ecology, fish health and stakeholder perceptions in Nile tilapia aquaculture in central Kenya

The paper investigates parasite infections in Nile tilapia aquaculture, finding that monopisthocotylan parasites are prevalent and linked to gill lesions, while stakeholders rarely identify parasites as a health cause.

Sex differences in cell type-specific transcriptional programs and GPCR signaling in the human subgenual anterior cingulate cortex

The paper identifies sex-specific transcriptional differences in specific cell types of the human subgenual anterior cingulate cortex and links them to GPCR signaling differences in mice.

Diversification of budding yeast polarization dynamics after compensatory experimental evolution

This study shows that evolution can restore cellular function without conserving microscopic organization, leading to novel molecular phenotypes in budding yeast.

A Binuclear Cluster Supports Functional Asymmetry in Dark-Operative Protochlorophyllide Reductase

This paper identifies a binuclear [Cu2S2] cluster in DPOR that mediates functional asymmetry between its active sites.

An open-access neuroimaging-compatible virtual town task for assessing human spatial navigation

The paper introduces an open-access virtual navigation task compatible with neuroimaging, demonstrating its use with EEG and behavioral tracking in human participants.

Structural basis for inefficient G protein coupling by Frizzled receptors

The paper identifies detergent LMNG as stabilizing a weak FZD-G protein interface, revealing that FZDs inherently weakly couple to G proteins and preferentially signal through DVL.

Genome evolution and glycosyltransferase diversification shape asperosaponin biosynthesis in Dipsacus asperoides

The paper reconstructs the asperosaponin biosynthesis pathway in Dipsacus asperoides by integrating genomics, transcriptomics, and biochemical analyses, revealing gene family diversification and glycosylation order.

Epigenetic reprogramming by promoter hypomethylation is linked to aberrant gene expression and transcriptomic plasticity in colorectal cancer

The paper identifies promoter hypomethylation as a driver of transcriptomic plasticity and aberrant gene expression in colorectal cancer, linking it to distinct cell states and metastatic behavior.

A multi-center retrospective chart analysis identifying clinical, biochemical, and radiographic signatures of short-chain enoyl-CoA hydratase (ECHS1) deficiency

This study identifies clinical, biochemical, and radiographic features of ECHS1 deficiency through a multi-center retrospective analysis of 113 patients, highlighting differences in onset and outcomes across age groups.

Prokaryotic homologs of the major vault protein form engineerable vault nanocapsules

The paper identifies prokaryotic homologs of the major vault protein that assemble into vault nanocapsules, revealing structural and size variability.

Temperature alters Culex tarsalis West Nile virus vector competence, tissue bottlenecks, and transcriptional responses

This study shows that temperature affects Culex tarsalis vector competence, tissue bottlenecks, and gene expression in response to West Nile virus infection.

Dynamic allocation of MurJ coordinates bacterial growth and division

The paper shows that MurJ, a Lipid II flippase, dynamically coordinates bacterial growth and division by transiently capturing at division sites during septal synthesis.

RNA Polymerase Backtracking Promotes Type III-A CRISPR DNA Targeting

The paper shows RNA polymerase backtracking promotes type III-A CRISPR DNA targeting by inhibiting GreA anti-backtracking factors, which suppress Cas10-Csm activity.