Bacterial Genetics and Biotechnology

The paper identifies an antisense RNA, ArrA, that inhibits DnaA production in Bacillus subtilis and affects sporulation timing.

This study shows that ciprofloxacin induces DNA supercompaction in E. coli, requiring RecN and dependent on RecA, as part of a DNA damage response.

This study shows that cyanoexosortase B (CrtB) is essential for motility, biofilm formation, and scytonemin production in Nostoc punctiforme.

The paper identifies regulatory elements and transcription factors in E. coli genes across diverse environments using experimental and computational methods.

This study identifies MutT4 in Mycobacterium tuberculosis as the RppH homolog, showing it forms condensates that facilitate mRNA degradation and enhance stress resistance.

This study links DnaA activity oscillations to DNA replication initiation in E. coli, showing they persist independently of dnaA expression and proposing an extrusion model for regulation.

This study identifies a novel non-catalytic function of PcrP, a PA2803 subfamily protein, which contributes to polymyxin B resistance in P. aeruginosa through protein interactions and Ca2+-dependent mechanisms.

The paper identifies and validates novel plasmid origins of replication from an environmental Pantoea strain, enabling expression of heterologous proteins and pathways in both model and non-model bacteria.

This study identifies dihydropyridines as inhibitors of Brucella ovis growth and shows that pseudogenes like bepE can confer stress resistance through functional restoration.

This study shows that acetylation of HBsu at K41 regulates gene expression during sporulation in Bacillus subtilis, affecting spore resistance and timing.

This paper identifies a dual-binding mechanism by which ZapC crosslinks FtsZ filaments, with the FtsZ C-terminal linker modulating interactions with ZapC and other proteins.

This study identifies YohP as a small membrane protein in E. coli that induces membrane depolarization and (p)ppGpp accumulation, leading to metabolic silencing under stress.

This study combines live cell fluorescence imaging and in situ cryo electron tomography to investigate the septation process in Deinococcus radiodurans, revealing insights into cell envelope architecture and molecular mechanisms of septal growth and closure.

The paper develops Swarm-seq to assess CsrA homologs' ability to regulate swarming motility, revealing functional divergence in RNA-binding specificity across bacterial species.

The paper identifies rapidly evolving orphan immunity genes in human gut bacteria that protect against T6SS intoxication through high-affinity protein interactions and shows their evolutionary dynamics across microbiomes.

This study presents cryo-EM structures of Mor-TAC and C-TAC complexes, revealing a tetrameric binding mode and interactions with RNAP, elucidating the transcription activation mechanism of phage Mor/C family activators.

This paper shows that a single amino acid change in the EnvZ-OmpR system improves ethanol tolerance in E. coli by stabilizing the outer membrane and altering iron homeostasis.

This study shows Pseudomonas aeruginosa chemotactically responds to serotonin, dopamine, epinephrine, and norepinephrine, with TlpQ chemoreceptor involvement, and highlights their role in virulence.

This study identifies SanA as an inner membrane protein in Salmonella that influences antibiotic resistance and infection phenotype by regulating SPI-I expression during growth phases and host cell entry.

The paper develops a biophysical model to elucidate how Spo0A[~]P binding to its regulatory boxes affects spo0A transcription in Bacillus subtilis, revealing repressive effects and the role of RNAP holoenzyme levels in expression increase.

This paper investigates how DNA binding proteins evolved sequence specificity by analyzing the IHF/HU family and reconstructing ancestral sequences.

This study uses Tn-seq to identify metal resistance genes in Pseudomonas putida, revealing both known and novel genes involved in cadmium, copper, and cobalt resistance.

This study uses RNA-sequencing to analyze E. coli's transcriptional response to hypothiocyanite, identifying rclB and rclC as potential regulators of membrane permeability.

This study identifies a SNARE-like mechanism in Myxococcus xanthus that mediates outer membrane exchange through TraA and TraB proteins, revealing parallels to eukaryotic SNAREs.

The paper shows that faster drug concentration changes reduce persister cells in E. coli, suggesting that pharmacokinetics affects persister formation.

The paper identifies cis-regulatory elements and transcription factors regulating the cps locus in Neisseria meningitidis, revealing the role of HTH_XRE in promoter function.

This study elucidates the mechanism of MBOAT-SGNH acyltransferases in Gram-negative bacteria, showing that a tyrosine-containing peptide shuttles acyl groups from MBOAT to SGNH active sites for peptidoglycan O-acetylation.

This paper investigates the role of teichoic acids in maintaining the periplasmic space in Streptococcus pneumoniae using cryo-electron microscopy and metabolic labeling, revealing new insights into TA composition and function.

This study investigates the unique signaling mechanisms of the RT-HK kinase in the GSR network of Rubellimicrobium thermophilum, revealing inverted logic and specific downstream interactions.

The paper measures translation rates and transcriptomes in single bacterial cells to identify a subpopulation with increased protein translation and specific gene overexpression under nutrient limitation.