Reproductive Biology and Fertility

This study creates a cross-species single-cell transcriptomic atlas of early ovarian development in cattle, human, and mouse, identifying shared and species-specific cell types and genes.

This study shows that inhibiting JNK in oocytes protects against chemotherapy-induced infertility and ovarian hormone loss in mice.

This study shows glucose regulates trophectoderm differentiation in bovine embryos through Hippo signaling via HBP and PPP, not glycolysis.

This study shows that chronic unpredictable mild stress induces age-dependent ovarian aging and POI in young and middle-aged female mice, with distinct endocrine and transcriptomic responses.

This study shows that adding L-proline to embryo culture media increases intracellular glutathione levels, reducing oxidative stress in preimplantation mouse embryos.

This paper validates a CUBIC-based workflow for volumetric analysis of follicles and vasculature in human ovarian tissue, showing comparable follicle density to histology and detailed vascular morphometrics.

The paper identifies GPLD1 as the enzyme mediating JUNO shedding in porcine oocytes, which is critical for preventing polyspermy and improving IVF outcomes.

This study identifies NFYA as a regulator of two sequential transcriptional activation events during oocyte-to-embryo transition, showing its role in primordial follicle activation and zygotic genome activation.

This study identifies selective transcriptomic vulnerabilities in neural tissues during vitrification, showing under-representation of membrane-integrated transcripts in the hippocampus.

This paper introduces a rapid in vitro system for analyzing maternal effect genes during mouse oocyte growth using microinjection and culture, showing effective gene manipulation without compromising developmental competence.

This study reanalyzes single-cell RNA sequencing data to show that mitochondrial gene expression changes precede oxidative phosphorylation activation and lineage specification in human pre-implantation embryos.

This study shows ovarian macrophages regulate germ cell meiotic entry, vascular growth, and germ cell clearance during oocyte reserve establishment.

This study develops a quantitative framework to distinguish between clock and domino mechanisms in embryogenesis, finding domino governs cleavage timings while a clock mechanism governs morula and blastocyst timings.

The paper challenges the notion of synchronous meiosis initiation in mouse fetal ovaries, showing asynchronous entry and progression from E12.5 to E16.5, supported by a predictive meiotic clock model.

This study uses single-cell proteomics to identify protein changes in human oocytes during in vitro aging, revealing key biological processes and potential biomarkers.

This study compares in-vivo and in-vitro mouse embryo development using 4D live imaging, finding that in-vitro culture delays development, alters morphology, and disrupts cell lineage specification.

The paper shows that lowering insulin levels in mice prevents reproductive aging and diet-induced ovarian dysfunction, linking hyperinsulinemia to subfertility.

This study shows that ZP2, a zona pellucida protein, is re-synthesized and functionally required in mouse embryos, playing an active role in blastocyst formation and cell lineage development.

This study shows that MAP hydrogel encapsulation improves revascularization and endocrine function of transplanted ovarian tissue in a mouse model of primary ovarian insufficiency.

This study examines the early transcriptomic changes in mouse ovaries after cyclophosphamide exposure, identifying p53-driven gene expression patterns linked to apoptosis and DNA damage.

This study identifies the DGAT1-PANK2-NRF2 axis as critical for lipid droplet remodeling, redox balance, and oocyte quality, with dysfunction linked to age-related decline.

The paper defines ovarian fibrosis as collagen accumulation and anisotropic organization in mouse and human ovaries using polarized light microscopy.

The paper identifies mRNA isoform switching as critical for mural and cumulus granulosa cell differentiation in mice by analyzing transcriptomes and differential transcript isoform expression.

The paper identifies newly evolved endogenous retroviruses (ERVs) as drivers of epigenetic priming in non-growing oocytes, enabling follicular activation during oogenesis.

This study maps over 85,000 oocytes in mouse ovaries across their reproductive lifespan, revealing spatial patterns and a bimodal size distribution, along with a mathematical model of oocyte activation and loss.

The paper uses nanoCT and confocal imaging to show symmetric follicle organization in mouse ovaries across lifespan, preserved despite follicle health or atresia.

This study shows that PIWIL3 is essential for rabbit oogenesis and embryogenesis by regulating transcriptome and proteome, with implications for human fertility.

This study shows that apoptosis in C. elegans maintains germline structure and fertility by preventing morphological defects and ensuring proper oocyte development.

This study screens compounds to identify those that block meiotic progression in mouse oocytes, offering potential non-hormonal contraceptive candidates.

This study analyzes ovarian aging across species using single-cell RNA-seq data to identify conserved and species-specific features and suggests drug repurposing candidates.