Reproductive Biology and Fertility

The paper investigates PADI6's role in oocyte cytoplasmic lattices using single-cell proteomics and transcriptomics, revealing its critical scaffolding function and connections to other oocyte compartments.

The paper generates an ovarian-specific FLAG-ICER-II(C) transgenic mouse model and shows it results in hyper-ovulation upon gonadotropin stimulation.

The paper shows that growing mouse oocytes receive mRNAs from granulosa cells, which are enriched for transcripts important for oocyte maturation and early embryo development.

The paper uses live imaging and single-cell sequencing to show that oocyte competition in mice enhances quality through autophagy-driven debris absorption, essential for fertility.

This study shows that TAF4b is essential for coordinating meiotic prophase I progression and early oocyte differentiation in mouse fetal oocytes.

This study compares blastocyst formation rates of human embryos cultured in media with varying pH and composition, finding similar rates between uterine-mimicking and standard media.

This study measures uterine temperature, pH, and fluid composition in humans three days after a positive LH test or ovum pick-up, finding lower pH and specific metabolite concentrations compared to in vitro culture conditions.

This study identifies eIF4ET's role in maintaining oocyte proteome stability during long-term storage by forming condensates, with implications for age-related infertility.

This study shows that knocking out RGS2 in CD4+ T cells increases oocyte numbers and alters immune cell populations in the reproductive organs of mice.

This paper uses Raman imaging and cluster analysis to study cytochrome c's role in mitochondrial metabolism of human oocytes across different maturation stages, finding it essential for respiration and apoptosis.

This study uses single-cell RNA-seq to analyze transcriptomic changes during the maternal to zygote transition in mice and rats, revealing similar but distinct transcriptional programs in both species.

This study shows that the microbiota influences reproductive lifespan by maintaining the ovarian reserve through short chain fatty acids and gene regulatory networks.

The paper shows that some oocytes from the first juvenile wave pause growth and later resume, contributing to fertility beyond puberty.

This study identifies dysregulated epigenetic regulators and transcription factors in ErβKO rat ovaries during postnatal development, leading to altered follicle activation and downstream gene expression.

The paper shows that asexual haploid blastocyst fitness varies across mouse strains, with selectively-inbred mice showing higher success rates in generating haploid blastocysts compared to outbred mice.

This study uses single-cell transcriptomics to analyze mouse ovarian aging and identifies molecular changes associated with the estropausal transition.

This study shows that dry incubators with time-lapse monitoring support bovine embryo culture with comparable morphokinetics and transcriptomic profiles to humidified incubators.

This study identifies RNA polymerase II degradation as the driver of chromatin reorganization from non-surrounded nucleolus to surrounded nucleolus in mammalian oocytes.

This study compares gene expression in human embryos cultured in time-lapse systems versus conventional incubators and identifies molecular mechanisms linking morphokinetic parameters to embryo development.

This study shows that theca cells regulate ovarian follicle growth through mechanical signaling and tissue pressure, affecting granulosa cell behavior and oocyte development.

The study shows that step-by-step autologous ovarian transplantation improves longevity in mice more effectively than hormonal replacement therapy.

The paper identifies 3,033 potential new genes in cattle oocytes and embryos using transcriptome sequencing and validates one gene's role in blastocyst development through CRISPR-Cas9.

This study investigates the dose-response effects of FSH on mouse folliculogenesis using single-cell RNA sequencing, revealing gene expression patterns and potential molecular mechanisms of FSH-induced ovarian disorders.

This study investigates how kidney tonifying and liver soothing methods affect mouse oocyte quality by regulating BMP-6 and Smads pathways through protein and mRNA expression analysis.

This study identifies CDK1/MAPK and AURKA/PLK1 pathways as converging on CPEB1 phosphorylation during mouse oocyte meiosis, showing CDK1 is critical for translation activation.

The paper shows that the orientation of the first cleavage in mouse oocytes follows the shorter diameter of the unfertilized oocyte, leading to segregation of animal and vegetal hemispheres and explaining differences in monozygotic twins.

This study shows that age-related structural changes in the zona pellucida reduce fertilization rates in mice by impairing cumulus-oocyte interactions and sperm binding.

This study develops a low-invasive electroporation method for mRNA delivery into mouse oocytes, achieving high survival rates and enabling chromosomal dynamics visualization.