Microtubule and mitosis dynamics

This study identifies GMCL1 as a negative regulator of the mitotic stopwatch pathway, showing it promotes 53BP1 degradation and reduces paclitaxel sensitivity in cancer cells.

The paper presents structural studies of the archaeal CdvAB cell division machinery, showing CdvA forms helical filaments and CdvB adopts ESCRT-III-like folds, revealing membrane engagement mechanisms.

This study identifies Annexin A2 and lamin B-containing biomolecular condensates in mitotic cells that facilitate membrane recycling and partitioning of endomembranes during cell division.

This study shows that multiple clustered centrosomes in antigen-presenting cells enhance T cell activation by positioning near the cell center, unlike T cells where centrosomes polarize toward the immune synapse.

This study identifies two recruitment pathways for HJURP to centromeres via CENP-C and Mis18C, essential for CENP-A deposition and centromere maintenance in chicken cells.

This study shows tau protein binds DNA, forms condensates, and localizes to centromeres, suggesting a new role in mitosis and cell division.

This paper reports high-resolution structures of kinesin-14 motor proteins during different mechanochemical states, revealing structural transitions that drive force generation and the power stroke.

The paper determines cryo-EM structures of tubulin cofactors bound to β-tubulin, revealing a vise-like mechanism for GTP-hydrolysis dependent α/β-tubulin biogenesis.

This paper identifies Aurora B-mediated phosphorylation and acetylation of BubR1 as critical for SAC signaling, linking kinetochore attachment to checkpoint activity and offering therapeutic targets for chromosome instability cancers.

This study identifies a mechanism by which Cdc5 and Cdc14 coordinate to resolve DNA catenanes through regulation of Topoisomerase II during mitosis.

This study creates kinesin-6 and kinesin-14 chimeras in fission yeast to show that domain functions dictate motor localization, directionality, and force production, leading to aneuploidy.

The paper identifies COP9 signalosome and PRMT5 methylosome complexes as regulators of Lis1-dynein transport, showing their opposing roles in dynein activation and stalling.

This study investigates cellularization in chytrid fungi, showing it uses distinct mechanisms from conventional cytokinesis and animal yeast cellularization, involving actomyosin-driven membrane invagination and microtubule-dependent patterning.

This study identifies HMMR's role in stabilizing the SACK1D-CK1 complex at the mitotic spindle and shows how phosphorylation drives its degradation after mitosis.

The paper determines cryo-EM structures of Arabidopsis thaliana Augmin, revealing its coiled-coil assembly, antiparallel dimerization, and NEDD1 binding mechanisms.

This study shows that RB regulates centromere transcription during mitosis, and its loss leads to increased RNA Pol II activity, DNA breaks, and mitotic defects.

The paper introduces a high-throughput 3D culture system for imaging axonal processes in human iPSC-derived neurons, enabling efficient study of microtubule dynamics and cargo transport.

This study identifies BICD2 mutations linked to SMALED2 that cause dynein hyperactivity and interactome changes, contributing to disease pathology.

The paper measures microtubule mechanics in living cells using motor-generated forces and shows that polyglutamylation increases microtubule stiffness.

This study identifies a mutation in the vesicular acetylcholine transporter (UNC-17) that increases tubulin acetylation, impairing axonal transport by disrupting interactions between UNC-104, UNC-17, and MEC-17.

The paper shows that the SxIP motif binds EBH with low affinity, but full interaction requires post-SxIP residues, leading to a nanomolar affinity and enhanced recruitment to microtubule plus ends.

This study identifies SUMOylation sites in KNL2 that are critical for its centromere targeting and interaction with CENH3 in Arabidopsis.

This study shows that cytoplasmic actomyosin fluctuations enhance dynein-driven transport in C. elegans embryos by accelerating motor-cargo movement through asymmetric force responses.

This study investigates how MCAK binds to growing microtubule ends using single-molecule imaging, revealing its nucleotide-dependent binding mechanism and interaction with other end-binding regulators.

This study investigates how stable microtubules reorient during neuronal development in rat hippocampal neurons, revealing a shift from plus-end-out to minus-end-out orientation.

The paper shows synaptic vesicles from mouse brains can sequester soluble tubulin in vitro, forming tubulin-rich regions.

This study identifies BSL1 as a plant-specific phosphatase that dephosphorylates CDKB1 to promote mitotic entry by counteracting phosphorylation at T14/Y15 residues.

This study determines cryoEM structures of human separase in apo and substrate-bound forms, revealing substrate recognition mechanisms and cleavage regulation.