Microtubule and mitosis dynamics

Shepherding by biased diffusion organizes diffusible proteins on microtubules

The paper demonstrates that motors can shepherd diffusive proteins on microtubules through biased diffusion, revealing a mechanism for spatial patterning without direct binding.

Yra2 regulates proteolysis of Cse4 to prevent its mislocalization to non-centromeric regions for chromosomal stability in budding yeast

This paper identifies Yra2's role in ubiquitin-mediated proteolysis of Cse4 to prevent its mislocalization and maintain chromosomal stability in budding yeast.

Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity

The paper shows that Eg5 organizes antiparallel microtubule bundles in human metaphase spindles through density-driven steric interactions, independent of spindle bipolarity.

Kinase interplay centered on two regulatory elements compartmentalizes the checkpoint protein Lte1 in S. cerevisiae

The paper identifies two regulatory elements in Lte1 that coordinate kinase activity to compartmentalize Lte1 at the bud cortex in S. cerevisiae, ensuring proper mitotic exit.

Divergence of Cortical Force-Generating Mechanisms Underlies Differences in Spindle Behavior between C. elegans and C. inopinata

This study compares spindle behavior in C. elegans and C. inopinata embryos, finding differences in pronuclear migration and spindle oscillations linked to changes in cortical force generation.

Microtubules sustain the fidelity of cellularization in a coenocytic relative of animals

This study investigates cellularization in Sphaeroforma arctica, showing microtubules help guide plasma membrane furrows to ensure equal partitioning of nuclei and cytoplasm during division.

Septins regulate cytokinesis and multicellular development in the closest living relatives of animals

This study shows that septins regulate cytokinesis and multicellular development in Salpingoeca rosetta, a choanoflagellate closely related to animals.

A Distinct Interphase Microtubule State Marks Host Cell Permissiveness to Chlamydia pneumoniae Entry

Auxin-inducible degron-mediated protein depletion in Diplonema papillatum

This study introduces an auxin-inducible degron system in Diplonema papillatum to enable conditional protein depletion and assesses its effects on key cellular processes.

Higher frequency of homologous chromosome pairing in adult endothelial cells as compared to neonatal endothelial cells

The paper compares homologous chromosome pairing in adult and neonatal endothelial cells, finding higher pairing frequency in adults.

Differential contributions of β tubulin isotypes to acentrosomal oocyte meiosis in C. elegans

The paper investigates how β-tubulin isotypes TBB-1 and TBB-2 contribute to acentrosomal spindle bipolarity and function in C. elegans oocytes.

Multiple evolutionary routes to cytoskeletal arborization revealed by the rhizarian amoeba Filoreta ramosa

This study investigates cytoskeletal arborization in the rhizarian amoeba Filoreta ramosa, revealing multiple evolutionary routes to similar branched cellular architectures.

Therapeutic Stress-induced Activation of PGCC Life Cycle Drives the Resistance Acquisition and Structured Tissue Differentiation

This study tracks how therapeutic stress activates a PGCC life cycle, leading to resistance and tissue differentiation in cancer cells.

Greatwall Kinase regulates Acute Myeloid Leukaemia Cell Division through a Non-Canonical Mechanism

This study shows Greatwall kinase regulates AML cell division through a non-canonical pathway distinct from its role in solid tumors.

Excess p50 induces Arp1-dependent dynactin clusters containing the assembly factor VezA

The paper shows that excess p50 induces Arp1-dependent dynactin clusters containing VezA in A. nidulans, revealing a role for p50-Arp1 interactions in trapping VezA.

Tubulin C-terminal tails are pH sensors that regulate microtubule function

The paper investigates how tubulin C-terminal tails respond to pH changes and shows their role in regulating microtubule interactions through protonation of glutamate residues.

Whole genome duplication through mitotic slippage causes nuclear instability

The paper investigates how different routes to whole-genome duplication (WGD) affect nuclear stability, finding that mitotic slippage uniquely causes nuclear instability.

Distinct Tubulin C-Terminal Tails Control the Efficiency of a Microtubule Severing Machine

This study investigates how different tubulin CTT isotypes influence katanin structure and dynamics, revealing that inhibitory CTTs enhance pore narrowing and flexibility, while non-inhibitory CTTs disrupt coordination.

Live dynamics of induced cell-cell fusion between mitotic and interphasic cells

This study uses cell-cell fusion to investigate how mitotic and interphase nuclei interact in shared cytoplasm, revealing checkpoint override events and plasticity in cell cycle control.

Paclitaxel induces NM2-dependent cellular contraction through GEF-H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

This study shows paclitaxel induces cancer cell contraction via GEF-H1 dissociation from microtubules and RhoA/ROCK activation, with tubulin acetylation regulating this process.

Nim1-related kinases regulate septin organization and cytokinesis by modulating Hof1 at the cell division site

This study identifies roles for Elm1 and Gin4 in septin organization and cytokinesis, revealing their interactions with Hof1 and kinase-independent functions of Hsl1 in yeast.

The CENP-A chaperone complex spatially organizes centromeres

The paper shows that the CENP-A chaperone complex forms shell-like structures around centromeres in interphase, essential for centromere architecture and genome stability.

The native structure of the Trichonympha centriole cartwheel reveals a zigzag stacking pattern

The paper resolves the native structure of the Trichonympha centriole cartwheel using cryo-electron tomography, revealing a zigzag stacking pattern of SAS-6 tetramers and their role in structural stability and polarity.

Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division in C. elegans

The paper investigates the regulation of cortical dynein and its interaction with microtubules during mitosis in C. elegans by analyzing the effects of depleting complex components and regulators.

Spindle Assembly Checkpoint Competency Determines Sensitivity to KIF18A Inhibition in Small-Cell Lung Cancer

This study identifies SAC competency as the determinant of sensitivity to KIF18A inhibition in SCLC, offering a predictive biomarker for targeted therapies.

Stepping dynamics of dynein characterized by MINFLUX

This study uses MINFLUX to track yeast dynein stepping dynamics at high resolution, revealing ATP hydrolysis per step and 8 nm steps with transient plus-end movements.

How Phosphorylation of alpha/beta-Tubulin Perturbs Microtubule Structure: A Computational Study

This study uses molecular dynamics simulations to show how phosphorylation of alpha or beta-tubulin alters microtubule structure and affects GTP hydrolysis and dynamic instability.

Prefoldin function links meiotic chromosome segregation with cellular remodeling and reveals tubulin sensitivity of the meiotic spindle

This study identifies Gim3 as a critical regulator of meiotic chromosome segregation in budding yeast, revealing its role in spindle sensitivity to tubulin levels and meiotic cellular remodeling.

Polo-like kinase phosphorylation of the orphan kinesin KIN-G negatively regulates centrin arm biogenesis in Trypanosoma brucei

This study identifies TbPLK-mediated phosphorylation of KIN-G as a negative regulator of centrin arm biogenesis in Trypanosoma brucei.

Lamin B1-dependent regulation of human separase in mitosis

This study identifies lamin B1 as a regulator of separase activity during mitosis, showing that lamin B1 depletion or overexpression disrupts chromosome separation and centrosome stability.