Fungal and yeast genetics research

The paper screens yeast mutants to identify genes that enhance recombinant laccase secretion, finding that vesicle trafficking and chaperone proteins are critical for improved protein production.

This study identifies the maiA gene in Aspergillus fumigatus as critical for cell wall homeostasis and virulence through in vitro and in vivo experiments.

This study investigates the role of the osaA gene in Aspergillus fumigatus, showing its impact on growth, conidiation, cell wall integrity, secondary metabolism, and virulence.

This paper develops and validates a CRISPR activation system in Nakaseomyces glabrata to study gene overexpression in pathogenesis and drug resistance.

The paper introduces a method using the chitin:DNA ratio to estimate fungal growth form, validated in Zymoseptoria tritici and Fusarium oxysporum.

This study profiles the effects of SAM1 and SAM2 gene deletions on S. cerevisiae by analyzing growth and gene expression changes across multiple conditions.

This study identifies Gdh2 and its regulatory mechanisms in Candida albicans for amino acid-dependent environmental alkalization, showing its role in virulence and microbial community dynamics.

This study identifies calcineurin-dependent genes and pathways critical for fitness in Candida glabrata under various stress conditions, highlighting roles in cell wall biosynthesis and vesicular trafficking.

The paper improves the Z3EV promoter system to create the strongest yeast promoter, P36, which is 1.4 times stronger than TDH3 and can express up to 50% of total protein as a heterologous protein.

This paper expands the YESS system by creating integrative plasmids for modular and uniform activity reporting of PTM-enzymes in yeast.

This paper examines the genome of Schizosaccharomyces japonicus var. versatilis isolate CBS5679, showing it diverged from S. japonicus 25 million years ago and has genomic differences preventing viable progeny with the reference strain.

This paper introduces CReATiNG, a method to build synthetic chromosomes from natural DNA in yeast, enabling chromosome recombination, modification, and deletion of non-adjacent regions.

The paper identifies recombination hotspots in Neurospora crassa controlled by idiomorphic sequences and meiotic silencing, showing dominant suppression is due to silencing of the active allele.

This paper describes a stable linear artificial chromosome in Komagataella phaffii that can carry multiple genes for biomanufacturing without disrupting the native genome.

This paper describes a method to efficiently generate heterothallic fission yeast strains using CRISPR-Cas9 and a mating type locus alteration.

This study identifies structural variants in a domesticated Lachancea cidri yeast strain associated with improved fermentation traits compared to wild isolates.

The paper estimates heritability of gene expression traits in yeast hybrids and finds non-additive components account for 36% of phenotypic variance.

This study shows that Zta1, a quinone reductase in C. albicans, enhances resistance to oxidative stress and works with flavodoxin-like proteins to protect against quinones.

The paper expands mitochondrial genome sampling across Saccharomycotina yeasts by extracting sequences from nuclear genome datasets, revealing trends in size, GC content, and gene presence.

FUN-PROSE predicts condition-specific gene expression in fungi using promoter sequences and transcription factor data, achieving high correlation with observed expression.

The paper constructs a synthetic disomic yeast using SCRaMbLE and a newly synthesized chromosome to study aneuploidy phenotypes, finding that both copy number and specific chromosomal regions affect fitness.

The study compares heat shock responses across Saccharomyces species and hybrids to assess thermal tolerance divergence.

The paper uses CRISPR D-BUGS and chromosome swapping to identify and resolve genetic interactions in synthetic chromosomes, revealing unexpected links between transcription, inositol metabolism, and tRNA abundance.

The paper investigates how Tra1 regulates azole resistance in different yeast species, finding species-specific effects on gene expression and drug tolerance.

The paper describes an efficient PCR-based method for gene targeting in wild-type Debaryomyces hansenii isolates, achieving high integration rates through homologous recombination.

This study maps quantitative trait loci (QTL) for osmotic stress response in Zymoseptoria tritici, identifying candidate genes and QTL associated with salt tolerance.

This paper presents an optimized electroporation protocol for high-efficiency yeast transformation, enabling efficient expression of whole-genome libraries with minimal DNA input.

The paper identifies two mechanisms that restrict SBF activity during meiotic entry in budding yeast, highlighting their role in regulating gene expression and meiotic progression.

The paper shows that Cdc42 interacts with Axl2 and Bud3 to recruit septins during axial budding in yeast.