Innovative Microfluidic and Catalytic Techniques Innovation

Microfluidic-enabled production of DNA barcoded APC library (MEDAL) for high throughput T cell epitope screening

The paper introduces MEDAL, a microfluidic platform for high-throughput T cell epitope screening by producing DNA-barcoded APCs loaded with peptides, enabling efficient identification of MHC-binding peptides.

Instant preparative droplet reaction assisted by shaking

The paper demonstrates a shake reaction method that significantly reduces reaction times for both organic and biological processes by using micro-nano droplets formed through shaking.

Label-free droplet image analysis with Cellprofiler

The paper presents a label-free image analysis pipeline using Cellprofiler to identify microfluidic droplets, microplastic particles, and bacterial growth without fluorescent labels.

AI-driven high-throughput droplet screening of cell-free gene expression

This paper introduces DropAI, an AI-driven droplet-based screening method to optimize cell-free gene expression systems, achieving cost reduction and yield improvement.

Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets

The paper presents a microfluidic platform that passively generates sub-microliter volumes of purified human sperm for vitrification, preserving sperm kinematics.

Systematic Investigation of Double Emulsion Dewetting Dynamics for the Droplet Microfluidic Production of Giant Unilamellar Vesicles (GUVs) under Biocompatible Conditions

This study investigates the factors affecting GUV production via double emulsion microfluidics and shows that high glycerol and P188 concentrations are unnecessary, enabling more reliable and accessible GUV production.

Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity

This paper develops a droplet microfluidic platform to screen ACE2 variants on native substrates, identifying K187T as a variant with fourfold increased catalytic efficiency.

Highly multiplexed community profiling of sub-nanoliter droplet-based co-cultures

The paper introduces Cocoa-seq, a microfluidic workflow for high-throughput analysis of microbial co-cultures, and benchmarks it using model communities and fluorescence microscopy.

High-throughput Monoclonal Antibody Screening from Immunized Rabbits via Droplet Microfluidics

This study develops a droplet microfluidics-based method to efficiently discover high-affinity rabbit monoclonal antibodies by overcoming B cell enrichment challenges.

Deterministic Cell Pairing with Simultaneous Microfluidic Merging and Sorting of Droplets

This paper introduces a microfluidic platform that deterministically pairs and sorts droplets containing cells, achieving high recovery of target cell pairs for studying cell crosstalk.

inDrops-2: a flexible, versatile and cost-efficient droplet microfluidics approach for high-throughput scRNA-seq of fresh and preserved clinical samples

The paper introduces inDrops-2, a cost-efficient scRNA-seq platform that profiles clinical samples with sensitivity comparable to commercial systems and demonstrates its utility in analyzing lung carcinoma samples.

Analyzing functional heterogeneity of effector cells for enhanced adoptive cell therapy applications

The paper demonstrates the use of double-emulsion droplet technology to analyze single-cell effector function in adoptive cell therapy, enabling high-resolution study of cell-cell interactions and functional heterogeneity.

Passive droplet microfluidic platform for high-throughput screening of microbial proteolytic activity.

This paper introduces a passive droplet microfluidic platform for high-throughput screening of microbial proteolytic activity, achieving 50-fold higher throughput than existing methods.

Drop-by-drop Addition of Reagents to a Double Emulsion

This paper introduces a device for picoinjection into double emulsions, enabling multistep assays by converting double emulsions to single emulsions on chip.

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

spinDrop improves single-cell sequencing by reducing noise and increasing detection rates, enabling high-quality molecular atlases of mouse brain development and organogenesis.

User-friendly analysis of droplet array images

The paper introduces user-friendly analytical pipelines for droplet array analysis using open-source tools and a custom visualization tool.

Models of Throughput for Multi-Cell, Multi-Type Droplet Microfluidics

The paper develops a statistical model to predict data throughput in multi-cell, multi-type droplet microfluidics experiments and identifies factors affecting its accuracy.

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

This paper develops a droplet-based platform for creating multicompartment artificial cells with remotely controllable protein channels using microfluidics and acoustic levitation.

Scalable and Automated CRISPR-Based Strain Engineering Using Droplet Microfluidics

The paper describes a microfluidic system for high-throughput CRISPR-based gene editing and screening, demonstrating its ability to perform parallel genetic modifications in E. coli.

Fluorinated oil-surfactant mixtures with the density of water: artificial cells for synthetic biology

The paper develops fluorinated oils with water-like density and surfactants to create stable droplets for synthetic biology applications.

A microfluidic platform for sequential assembly and separation of synthetic cell models

The paper develops a microfluidic platform that purifies and integrates the production of GUVs with their manipulation, achieving high efficiency in separating vesicles from residues.

Investigation of Different Free Image Analysis Software for High-Throughput Droplet Detection

This paper evaluates four free image analysis software for droplet detection, finding that objective-type software is more suitable for non-experienced users, with CellProfiler offering the best user experience.

Microfluidic Streptomyces Cultivation for Whole Lifecycle Characterization and Phenotypic Assays Enabled by Nanogap-stabilized Air-Water Interface

This paper introduces a microfluidic device to study Streptomyces lifecycle at the single-cell level, enabling observation of aerial hyphae development and phenotypic assays.

A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

The paper introduces a microfluidic system for single-cell transcriptome profiling and demonstrates its ability to identify cell cycle regulators.

CellProfiler: A fit tool for image analysis in droplet microfluidics

CellProfiler is a free open-source software tool for analyzing droplet microfluidic assays, demonstrated through droplet digital quantification of viable fluorescent bacteria.

Digital polymerase chain reaction in an array of microfluidic printed droplets

This paper introduces a cost-effective digital PCR method using microfluidic droplet printing on glass substrates, enabling reliable and affordable quantitative results.

Rapid microbial interaction network inference in microfluidic droplets

The paper introduces MINI-Drop, a method to infer microbial interactions in microfluidic droplets, showing it can accurately determine pairwise and higher-order interactions.

Microfluidic device for on-chip mixing and encapsulation of lysates

The paper introduces a microfluidic device with pneumatic valves for precise reagent injection and mixing in emulsion drops, demonstrating its use in DNA titration experiments for green fluorescent protein synthesis.

Droplet-based microfluidic analysis and screening of single plant cells

This paper demonstrates a droplet-based microfluidic system for analyzing plant protoplasts, enabling high-throughput phenotyping and gene expression analysis.