Innovative Microfluidic and Catalytic Techniques Innovation

Integrated microdroplet workflow for high-throughput cell-free transcription in double emulsion picoreactors

The paper introduces an integrated microfluidic workflow for high-throughput cell-free transcription using double emulsion picoreactors, enabling quantitative screening of regulatory sequences without specialized sorting equipment.

Development of a Microdroplet-Based Functional Genomic Screening pipeline by combination of DNA Nanoflowers and PURExpress Cell-Free Expression

The paper develops a microdroplet-based functional genomic screening pipeline using DNA nanoflowers and cell-free expression to identify DNA-interacting enzymes.

Scalable flow synthesis of ultrasmall inorganic nanoparticles for biomedical applications via a confined impinging jet mixer

This paper develops a scalable method to produce ultrasmall inorganic nanoparticles for biomedical use using a confined impinging jet mixer.

Fine-tuning cell-mimicking polyacrylamide microgels: Sensitivity to microscale reaction conditions in droplet microfluidics

This study shows that flow conditions in droplet microfluidics significantly affect the elasticity and size of PAAm microgels, despite identical monomer concentrations.

Post-Assay Photogelation Enables Flow-Cytometric Sorting and Recovery of Bacterial Cocultures from Microfluidic Droplets

This paper introduces post-assay photogelation to enable flow-cytometric sorting of bacterial cocultures from microfluidic droplets, achieving high-purity sorting and demonstrating its application in microbial community analysis.

Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries

This paper presents an automated microfluidic platform for generating large, precisely-defined lipid nanoparticle libraries and evaluates their physicochemical and transfection properties.

High-throughput single cell -omics using semi-permeable capsules

This paper introduces a semi-permeable capsule technology for high-throughput single-cell -omics, demonstrating its utility in scRNA-Seq of hematopoietic disorders and uncovering transcriptome changes in AML.

Deterministic co-encapsulation of microparticles in droplets via synchronized merging for single-cell genomics

The paper develops a method for deterministic co-encapsulation of microparticles in droplets for single-cell genomics, achieving accurate taxonomic profiling of microbial cells.

Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species

This study develops a droplet-based microfluidic platform for culturing plant protoplasts and evaluates its effectiveness across three species, finding tobacco protoplasts show highest viability.

Induced Droplet Ovalisation (IDO): Image-based microfluidic method for high-throughput and label-free characterization of microbial proteolytic strains from wastewater sludge.

This paper introduces a microfluidic method called IDO for high-throughput, label-free screening of microbial proteolytic activity from wastewater sludge, showing improved efficiency and sensitivity compared to traditional methods.

Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.

This paper improves the efficiency of FACS sorting of double emulsion picoreactors by optimizing surfactants and using a 3D-printed vertical mixer, achieving high accuracy and recovery in sorting.

SCO-pH: Microfluidic dynamic phenotyping platform for high-throughput screening of single cell acidification

SCO-pH enables high-throughput single-cell acidification analysis by compartmentalizing cells in microfluidic droplets and measuring extracellular pH changes to distinguish metabolic states.

Microfluidic-enabled production of DNA barcoded APC library (MEDAL) for high throughput T cell epitope screening

The paper introduces MEDAL, a microfluidic platform for high-throughput T cell epitope screening by producing DNA-barcoded APCs loaded with peptides, enabling efficient identification of MHC-binding peptides.

AI-driven high-throughput droplet screening of cell-free gene expression

This paper introduces DropAI, an AI-driven droplet-based screening method to optimize cell-free gene expression systems, achieving cost reduction and yield improvement.

Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets

The paper presents a microfluidic platform that passively generates sub-microliter volumes of purified human sperm for vitrification, preserving sperm kinematics.

Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity

This paper develops a droplet microfluidic platform to screen ACE2 variants on native substrates, identifying K187T as a variant with fourfold increased catalytic efficiency.

High-throughput Monoclonal Antibody Screening from Immunized Rabbits via Droplet Microfluidics

This study develops a droplet microfluidics-based method to efficiently discover high-affinity rabbit monoclonal antibodies by overcoming B cell enrichment challenges.

Deterministic Cell Pairing with Simultaneous Microfluidic Merging and Sorting of Droplets

This paper introduces a microfluidic platform that deterministically pairs and sorts droplets containing cells, achieving high recovery of target cell pairs for studying cell crosstalk.

inDrops-2: a flexible, versatile and cost-efficient droplet microfluidics approach for high-throughput scRNA-seq of fresh and preserved clinical samples

The paper introduces inDrops-2, a cost-efficient scRNA-seq platform that profiles clinical samples with sensitivity comparable to commercial systems and demonstrates its utility in analyzing lung carcinoma samples.

Analyzing functional heterogeneity of effector cells for enhanced adoptive cell therapy applications

The paper demonstrates the use of double-emulsion droplet technology to analyze single-cell effector function in adoptive cell therapy, enabling high-resolution study of cell-cell interactions and functional heterogeneity.

Passive droplet microfluidic platform for high-throughput screening of microbial proteolytic activity.

This paper introduces a passive droplet microfluidic platform for high-throughput screening of microbial proteolytic activity, achieving 50-fold higher throughput than existing methods.

Drop-by-drop Addition of Reagents to a Double Emulsion

This paper introduces a device for picoinjection into double emulsions, enabling multistep assays by converting double emulsions to single emulsions on chip.

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

spinDrop improves single-cell sequencing by reducing noise and increasing detection rates, enabling high-quality molecular atlases of mouse brain development and organogenesis.

User-friendly analysis of droplet array images

The paper introduces user-friendly analytical pipelines for droplet array analysis using open-source tools and a custom visualization tool.

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

This paper develops a droplet-based platform for creating multicompartment artificial cells with remotely controllable protein channels using microfluidics and acoustic levitation.

Scalable and Automated CRISPR-Based Strain Engineering Using Droplet Microfluidics

The paper describes a microfluidic system for high-throughput CRISPR-based gene editing and screening, demonstrating its ability to perform parallel genetic modifications in E. coli.

Fluorinated oil-surfactant mixtures with the density of water: artificial cells for synthetic biology

The paper develops fluorinated oils with water-like density and surfactants to create stable droplets for synthetic biology applications.

A microfluidic platform for sequential assembly and separation of synthetic cell models

The paper develops a microfluidic platform that purifies and integrates the production of GUVs with their manipulation, achieving high efficiency in separating vesicles from residues.

Investigation of Different Free Image Analysis Software for High-Throughput Droplet Detection

This paper evaluates four free image analysis software for droplet detection, finding that objective-type software is more suitable for non-experienced users, with CellProfiler offering the best user experience.

A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

The paper introduces a microfluidic system for single-cell transcriptome profiling and demonstrates its ability to identify cell cycle regulators.