Planarian Biology and Electrostimulation

Tig1 regulates proximo-distal identity during salamander limb regeneration

The paper identifies Tig1 as a regulator of proximo-distal identity during salamander limb regeneration through single-cell RNAseq analysis.

Somatic regulation of female germ cell regeneration and development in planarians

The paper identifies genes and factors involved in female germ cell development and regeneration in planarians, revealing shared molecular signatures with pluripotent stem cells and distinct somatic roles.

Manipulating the microbiome alters regenerative outcomes in Xenopus laevis tadpoles via lipopolysaccharide signalling

This study shows that the Xenopus laevis tadpole microbiome and lipopolysaccharide signaling influence tail regeneration outcomes, with TLR4 playing a partial role.

Acoel single-cell atlas reveals expression dynamics and heterogeneity of a pluripotent stem cell population

The paper characterizes stem cell dynamics and heterogeneity in the acoel Hofstenia miamia using single-cell transcriptomics, revealing lineage-primed neoblast subpopulations and their response to regeneration.

RNAi screen of RING/U-box domain ubiquitin ligases identifies critical regulators of tissue regeneration in planarians

The paper identifies critical RING/U-box E3 ubiquitin ligases involved in planarian tissue regeneration through RNAi screening and functional analysis.

Variable Shh and Fgf8 positioning in regenerating axolotl limb guarantees consistent limb morphogenesis in different limb sizes

The paper investigates how variable Shh and Fgf8 positioning in axolotl limb blastemas ensures consistent limb morphogenesis across different sizes.

Proliferation maintains the undifferentiated status of stem cells: the role of the planarian cell cycle regulator Cdh1

The paper shows that cell cycle arrest is necessary for stem cell differentiation in planarians, as inhibiting Cdh1 disrupts tissue homeostasis and regeneration.

Hox genes regulate asexual reproductive behavior and tissue segmentation in adult animals

This study identifies Hox gene functions in adult tissue segmentation and asexual reproduction in planarians, showing distinct roles for hox3 and post2b.

Neurotrophic control of size regulation during axolotl limb regeneration

This study identifies that limb nerves provide essential cues for regulating the final size of regenerating axolotl limbs during the 'tiny-limb' stage.

A powerful and versatile new fixation protocol for immunohistology and in situ hybridization that preserves delicate tissues in planaria

The paper introduces a new fixation protocol for immunohistology and in situ hybridization in planarians that preserves tissue structure and allows for successful signal development.

Transgenesis in the acoel Hofstenia miamia

This paper develops a transgenesis protocol for Hofstenia miamia, enabling stable transgene integration and tissue-specific fluorescent expression, revealing new insights into regeneration and cell biology.

Bacteria are required for regeneration of the Xenopus tadpole tail

The paper shows that bacteria are necessary for initiating tadpole tail regeneration by activating TLR pathways and sustaining NF-κB activity, which drives ROS production and regeneration.

Fluctuation of cellular differentiation in limb regeneration is regulated by Pde4b in urodele amphibians

This study shows that Pde4b regulates cellular differentiation during limb regeneration in urodele amphibians by modulating B2FF-induced plasticity in dermal fibroblasts.

Addiction-Related Memory Transfer and Retention in Planaria

This study investigates memory retention and transfer of addictive behavior in planaria, finding that addiction persists in both brain-containing and brainless posterior segments.

m6A-mediated Cell-cell Communication Controls Planarian Regeneration

This study shows that m6A-mediated cell-cell communication is essential for planarian regeneration, as disrupting m6A methyltransferase leads to regeneration failure.

TRiC activates the unfolded protein response and protects starved stem cells by modulating energy and lipid metabolism during planarian regeneration

This study identifies TRiC's role in maintaining stem cell function during planarian starvation by activating the UPR and regulating energy and lipid metabolism.

Signaling gradients in surface dynamics as basis for planarian regeneration

This paper develops a mathematical model to explain planarian regeneration by incorporating positional control genes and wnt-related signaling gradients, showing improved polarity preservation in regeneration.

Planarian stem cells sense the identity of missing tissues to launch targeted regeneration

This study shows planarian stem cells adjust their behavior based on missing tissue type, with pharynx regeneration requiring specific proliferation and ERK signaling, while eye regeneration does not.

Secreted inhibitors drive the loss of regeneration competence in Xenopus limbs

The paper identifies extrinsic cues from chondrogenic progression that inhibit AER cell formation and limit limb regeneration in Xenopus tadpoles.

Apoptosis is a generator of Wnt-dependent regeneration and homeostatic cell renewal in the ascidian Ciona

This study shows apoptosis and Wnt signaling are required for regeneration and homeostatic growth in Ciona, with apoptosis promoting progenitor cell survival.

A constitutively expressed fluorescence ubiquitin cell cycle indicator (FUCCI) in axolotls for studying tissue regeneration

The paper generates a transgenic axolotl line expressing FUCCI to study cell cycle dynamics during tissue regeneration.

Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiation

This study identifies and functionally characterizes five CBP genes in planarians, showing their roles in stem cell maintenance and differentiation.

CBP/p300 orthologs CBP2 and CBP3 play distinct roles in planarian stem cell function

The paper identifies two planarian CBP family members, CBP2 and CBP3, with distinct roles in stem cell maintenance and function.

DDX24, a D-E-A-D box RNA helicase, is required for muscle fiber organization and anterior pole specification essential for head regeneration in planarians.

This study identifies DDX24 as essential for planarian head regeneration by regulating muscle organization, pole specification, and Wnt signaling.

Single-cell analysis of Schistosoma mansoni reveals a conserved genetic program controlling germline stem cell fate

Formation and Spontaneous Long-Term Repatterning of Headless Planarian Flatworms

This study shows that ERK inhibition delays regeneration in headless planarians, but headless animals can later remodel to regain a single-headed morphology through Wnt/β-catenin signaling.

Hydrogen peroxide as regeneration-initiation signal that activates pERK to trigger planarian regeneration

The paper identifies hydrogen peroxide as an upstream signal initiating planarian regeneration by activating pERK and linking it to EGFR-MAPK/ERK pathway activation.

Decellularization enables functional analysis of ECM remodeling in planarian regeneration

This study uses decellularization, proteomics, and RNAi in planarians to identify ECM components essential for tissue homeostasis and regeneration.

A comprehensive conceptual and computational dynamics framework for Autonomous Regeneration Systems

This paper introduces a computational framework for damage detection and regeneration in multicellular systems, demonstrating its ability to regenerate tissues and stem cells in simulated organisms.

Single-Cell RNA-Seq Reveals Novel Mitochondria-related Musculoskeletal Cell Populations during Adult Axolotl Limb Regeneration Process

This study uses single-cell RNA sequencing to identify novel mitochondria-related musculoskeletal cell populations during axolotl limb regeneration.