Discover, rate, and discuss biology preprints.

This study shows that Greatwall kinase coordinates meiotic divisions by regulating PP2A-B55, which controls APC/C and Mos/MAPK pathways in Xenopus oocytes.

This study engineers HUH-tags to form sequence-directed covalent bonds with RNA, enhancing their reactivity through directed evolution.

The paper develops dsRADAR, a method using ADAR3-derived dsRNA-binding domains to detect and quantify dsRNA with higher sensitivity and specificity than existing antibodies.

The paper characterizes Cas12a-MG29-1, a metagenomics-derived nuclease, revealing structural and kinetic features that enhance its specificity and activity.

This study identifies the evolutionary emergence of redox sensitivity in vertebrate glucokinases through conformational expansion and cysteine residue acquisition.

This study reveals how APE1 uses a dynamic search mechanism combining 1D and 3D diffusion to efficiently locate and repair AP-sites in DNA.

This paper introduces Enriched-GF, a method for high-yield growth factor recovery from autologous blood, showing it outperforms existing methods in growth factor release and consistency.

The paper reports the structural and functional characterization of QatB and QatC proteins in the QatABCD anti-phage defense system, revealing their complex formation and potential regulatory roles.

This paper introduces a Sortase A-based method to farnesylate Cdc42 in vitro, enabling efficient and reproducible prenylation for biochemical studies.

This study identifies TBK1 as a regulator of IRGQ-mediated autophagy by disrupting the IRGQ-GABARAPL2 complex through phosphorylation of GABARAPL2.

This paper evaluates the potency and selectivity of MYST KAT inhibitors using chemoproteomic profiling and identifies new complex members, highlighting dose-dependent inhibition patterns and potential therapeutic applications.

This study evaluates β-glucanases as cell wall-permeabilising agents against Phytophthora agathidicida oospores, showing synergistic enzyme combinations enhance biocide effectiveness.

This paper develops a method to quantify chondroitin sulphate disaccharides from 25 microliters of human milk, enabling analysis of small-volume samples previously inaccessible to GAG profiling.

This paper introduces a high-throughput method for screening protein solubility using aqueous solvents, demonstrating its effectiveness with challenging de novo proteins.

This study shows HRS forms 2D condensates that drive flat clathrin lattice assembly on endosomes, with cholesterol enhancing this process.

This study investigates the impact of dysregulated lipid metabolism on the gut-brain axis in patients with intracerebral hemorrhage, finding associations between BPA exposure, oxidative stress, and increased risk of hypertensive ICH.

This study shows that substrate specificity in glucokinases is not linked to conformational diversity, as similar specificity ratios are observed across ancestral and extant forms with varying structural features.

This paper shows that combining proteasome inhibitors with lysosome-targeted degradation enhances cancer cell death by exploiting an adaptive autophagy response.

This study evaluates heterogeneous reconstruction algorithms in cryoEM using benchmark datasets with ground-truth labels, finding limited accuracy in particle classification despite resolving structural heterogeneity.

This study identifies HPCAL1 as a Ca2+-dependent regulator of AC8, revealing a new mechanism for cAMP signaling regulation.