J. T. Huiskonen

Native spike flexibility revealed by BSL3 Cryo-ET of active SARS-CoV-2 virions

This study uses cryo-ET to analyze native, active SARS-CoV-2 virions, revealing a transient open-trimer prefusion state and hidden spike flexibility with implications for vaccine design.

Structure and Interactions of the Endogenous Human Commander Complex

This study determines the structure of the endogenous human Commander complex using cryo-EM and proteomics, revealing its asymmetric organization and novel interactions involved in cellular processes.

Global analysis of aging-related protein structural changes uncovers enzyme polymerization-based control of longevity

The paper identifies age-related protein structural changes in yeast and shows that inhibiting polymerization of glutamate synthase extends lifespan.

DNA origami directed virus capsid polymorphism

This paper demonstrates DNA origami-directed polymorphism of viral capsids, showing control over shape, size, and topology through programmable assembly platforms.

Molecular view of ER membrane remodeling by the Sec61/TRAP translocon

The paper determines the structure of the Sec61/TRAP complex bound to a ribosome using Cryo-EM, revealing how TRAP facilitates ER membrane remodeling and polypeptide translocation.

Nanobody engineering for SARS-CoV-2 neutralization and detection

This study engineers nanobodies to neutralize SARS-CoV-2 variants and develop a detection assay, showing high potency against some variants but limitations against others.

Signal peptide mimicry primes Sec61 for client-selective inhibition

This study describes KZR-8445, a cyclic depsipeptide that selectively inhibits Sec61-mediated protein biogenesis by mimicking signal peptides, showing efficacy in a rheumatoid arthritis model.

Mechanistic insights into the activation of lecithin-cholesterol acyltransferase in therapeutic nanodiscs composed of apolipoprotein A-I mimetic peptides and phospholipids

This study investigates how apoA-I mimetic peptides modulate LCAT activity in nanodiscs through molecular dynamics simulations and experiments, revealing structural and functional insights.

New Vista into Origins of Viruses from a Prototypic ssDNA Phage

The paper characterizes a lipid-containing ssDNA phage, {Phi}CjT23, and suggests it represents a model for the last common ancestor of ssDNA and dsDNA viruses in the Bamfordvirae.

Structural basis underlying specific biochemical activities of non-muscle tropomyosin isoforms

The paper determines cryo-EM structures of actin filaments with two non-muscle tropomyosin isoforms, revealing structural differences that explain their distinct functional roles.

Intranasal inhibitor broadly blocks SARS-CoV-2 including recent highly immunoevasive Omicron subvariants

This paper describes a novel intranasal inhibitor, TriSb92, that effectively neutralizes SARS-CoV-2 and recent Omicron subvariants, offering protection against infection in mice.

Molecular rationale for hantavirus neutralization by a reservoir host-derived monoclonal antibody

This study investigates how a monoclonal antibody neutralizes hantaviruses by targeting the glycoprotein lattice, revealing structural insights into antibody binding and its impact on viral entry.

The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K

This study identifies TMEM16K as an ER-resident calcium-regulated lipid scramblase and elucidates its structural basis for lipid scrambling and inactivation.