N. E. Lewis

LeGenD: determining N-glycoprofiles using an explainable AI-leveraged model with lectin profiling

LeGenD predicts N-glycan structures and their abundance using lectin-binding patterns and AI, trained on glycoprofiles from recombinant proteins.

Glycoengineered recombinant alpha1-antitrypsin results in comparable in vitro and in vivo activities to human plasma-derived protein

This study generates glycoengineered recombinant alpha1-antitrypsin with in vitro and in vivo activities comparable to plasma-derived A1AT, offering a sustainable therapeutic alternative.

Dissecting the properties of circulating IgG against Group A Streptococcus through a combined systems antigenomics-serology workflow

This paper develops a combined systems antigenomics-serology workflow to characterize circulating IgG against Group A Streptococcus, revealing antigen repertoire and functional properties of antibodies in healthy and infected individuals.

Identification of hyperosmotic stress-responsive genes in Chinese hamster ovary cells via genome-wide virus-free CRISPR/Cas9 screening

The paper identifies hyperosmotic stress-responsive genes in CHO cells using CRISPR/Cas9 screening and validates their role in stress resistance and protein production.

Vascular proteome responses precede organ dysfunction in sepsis

This study identifies vascular proteome changes preceding organ dysfunction in sepsis using a murine model and integrative analysis.

The Human Milk Oligosaccharide 3-Sialyllactose Promotes Inflammation Resolution and Reduces Atherosclerosis Development in Mice.

This study shows that 3-sialyllactose reduces inflammation and atherosclerosis in mice by modulating transcriptional activity in macrophages.

What are housekeeping genes?

The paper defines housekeeping genes using experimental data and identifies conserved gene lists across multiple species.

Restoration of deficient DNA Repair Genes Mitigates Genome Instability and Increases Productivity of Chinese Hamster Ovary Cells

This study restores DNA repair genes in CHO cells, improving genome stability and protein titer.

Bacterial modification of the host glycosaminoglycan heparan sulfate modulates SARS-CoV-2 infectivity

This study shows that commensal bacteria modify heparan sulfate, reducing SARS-CoV-2 spike protein binding and that this bacterial presence decreases with age, increasing COVID-19 susceptibility.

Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor

The paper characterizes glycosylation of SARS-CoV-2 Spike and ACE2 using glycomics and simulations to understand their interactions and guide immunogen design.

A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes

The paper uses a CRISPR-Cas9 screen in Chinese hamster ovary cells to identify glutamine-sensitive genes, revealing a novel lipase involved in TCA cycle regulation.

Combating viral contaminants in CHO cells by engineering STAT1 mediated innate immunity

The paper identifies Gfi1 and Trim24 as repressors of STAT1 in CHO cells and shows that knocking them out increases resistance to RNA viruses.