Steven Lotz

Large-scale single-molecule analysis of tau proteoforms

This paper introduces IMaP, a method to analyze tau proteoforms at the single-molecule level, revealing ordered phosphorylation patterns and disease-specific modifications.

Multiomic Profiling of 85 iPSC Lines from Familial Dementia Reveals Cellular Diversity and Regulators of Organoid Development

The paper characterizes 85 MAPT mutation-bearing iPSC lines using multiomic approaches, revealing cellular diversity and factors influencing organoid development.

Single Cell Transcriptomics and Surface Protein Expression Reveal Distinct Cellular and Molecular Phenotypes in Human RPESC-RPE and PSC-RPE

The paper compares single-cell transcriptomic and surface protein profiles of RPESC-RPE and PSC-RPE, finding differences in gene expression and surface markers that suggest functional and immunomodulatory differences.

Inosine misincorporation into mRNA triggers the integrated stress response and activates an innate immune gene expression signature

This study shows that inosine misincorporation into mRNA activates the integrated stress response and an innate immune gene expression signature, linking ITPase deficiency to cellular stress.

MAPT Splicing Modulators as a Therapeutic Strategy for Tauopathies

Discovery of SMCs that correct MAPT splicing, reduce 4R tau, and rescue pathology in patient-derived neuronal and in vivo models of 4R tauopathies.

Fully Human Bifunctional Intrabodies Achieve Graded Reduction of Intracellular Tau and Rescue Survival of MAPT Mutation iPSC-derived Neurons

The paper develops fully human bifunctional anti-tau intrabodies that reduce intracellular tau and improve neuronal survival in disease-associated mutations.

Integrated Manufacturing Platform for Cortical Organoids Reveals Early Phosphatase and Prefoldin Dysregulation in MAPT V337M Neurons

The paper develops a scalable cortical organoid platform with quality control metrics and identifies early dysregulation of phosphatase and prefoldin genes in FTD-tau neurons.

Single Cell Profiling of CD45+ Spinal Cord Cells Reveals Microglial and B Cell Heterogeneity and Crosstalk Following Spinal Cord Injury

The paper uses single-cell RNA sequencing to identify immune cell populations and interactions in mouse spinal cord injury, revealing microglial and B cell heterogeneity and crosstalk.

Glutamatergic dysfunction precedes neuron loss in cerebral organoids with MAPT mutation.

This study shows that glutamatergic dysfunction occurs before neuron loss in cerebral organoids with MAPT mutations, suggesting therapeutic targets for FTD.

Cell-type specific in vitro gene expression profiling of stem-cell derived neural models

The paper extends the RiboTag system for cell-type specific RNA depletion in vitro, showing variable efficiency across replicates.