David Michael Lewinsohn

Human memory CD4+ T cells recognize non-infected macrophage bystanders exposed to Mycobacterium tuberculosis-infected cells

The paper shows human memory CD4+ T cells recognize both Mtb-infected and bystander macrophages, with some TCRs specifically recognizing infected cells.

Lung Resident T Cells Across the Spectrum of TB Infection and Disease

This study maps human pulmonary T-cell immunity across TB infection/disease using single-cell transcriptomics and functional T-cell cloning, identifying protective immune features and a platform for TB vaccine development.

Antigenic stimulation in conjunction with cytokine is required for mediating IL-17A production in human MAIT cells

This study shows that IL-23 and TCR stimulation together are needed for lung MAIT cells to produce IL-17A, with distinct transcriptional changes observed.

Sorting endosomes play key roles in presentation of Mycobacterium tuberculosis-derived ligands to MAIT cells

The paper identifies sorting endosomes as critical for MR1 antigen presentation of Mycobacterium tuberculosis-derived ligands to MAIT cells, highlighting the roles of Syntaxin 12 and 16 in this process.

Synaptotagmin 1 and Synaptotagmin 7 promote MR1-mediated presentation of Mycobacterium tuberculosis antigens

This study identifies Synaptotagmin 1 and 7 as facilitators of MR1-mediated antigen presentation of Mycobacterium tuberculosis to MAIT cells.

Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens

This study shows that mutations in MR1 outside the antigen binding groove differentially affect exogenous antigen presentation by altering B2M association.

Opposing roles for SNAP23 and SNAP25 in mediating MR1 trafficking and antigen presentation

This study identifies opposing roles for SNAP23 and SNAP25 in MR1 trafficking and antigen presentation to MAIT cells, showing SNAP23 enhances while SNAP25 suppresses antigen presentation.

BCG Vaccination at Birth Shapes the TCR Usage and Functional Profile of MR1T Cells at 9 Weeks of Age

This study examines how BCG vaccination at birth affects MR1T cell TCR usage and function in infants, finding increased type I interferon response and a TCR cluster enriched in vaccinated infants.

Disruption of riboflavin biosynthesis in mycobacteria establishes 5-amino-6-D-ribitylaminouracil (5-A-RU) as key precursor of MAIT cell agonists

Deleting early riboflavin genes (ribA2 in both species, ribG in M. smegmatis) sharply reduced MAIT cell activation, while ribH deletion raised recognition of M. tuberculosis only.

Selective inhibition of MR1-restricted T cell activation by a novel MR1-targeting nanobody

This study develops a high-affinity anti-MR1 nanobody that selectively inhibits MR1-restricted T cell activation, providing insights into MR1 function and a tool for immunological research.

Two distinct cytokine response clusters identified in healthcare workers with apparent resistance to infection with Mycobacterium tuberculosis despite sustained occupational exposure

The paper identifies two distinct cytokine response clusters in healthcare workers resistant to Mtb infection despite exposure, using cytokine profiling and clustering analysis.

Human Neonatal MR1T Cells Have Diverse TCR Usage, are Less Cytotoxic and are Unable to Respond to Many Common Childhood Pathogens.

This study compares neonatal and adult MR1T cells, finding neonatal cells have diverse TCR usage, reduced cytotoxicity, and limited pathogen recognition.

Modulation of riboflavin biosynthesis and utilization in mycobacteria

This study investigates riboflavin biosynthesis and utilization in mycobacteria by creating conditional knockdowns and analyzing their impact on viability and MAIT cell recognition.