Neal M. Alto

Targeting of ELMO2 by the Shigella E3 Ligase IpaH4.5 Reveals Key Differences Between ELMO Paralogs

The paper identifies ELMO2 as a specific target of Shigella IpaH4.5 ubiquitin ligase, revealing divergence in ELMO paralogs and their functional roles in bacterial infection.

Intracellular pathogen targeting by IL32 elicits cell-autonomous immunity

The paper identifies IL32 as a cytokine that targets intracellular pathogens via the N-degron pathway and autophagy, revealing a mechanism by which IFN-gamma signaling mediates host defense against pathogens like Chlamydia.

Phosphoinositide regulation by the CCC complex promotes phagosome maturation and host defense

This study shows that the CCC complex regulates PI(3)P levels on phagosomal membranes, which is critical for phagosome maturation and antibacterial immunity.

Bacterial and human exonucleases mediate interkingdom antiviral immunity

This study identifies ISG20 and its bacterial homologs as interkingdom antiviral factors that restrict both bacterial RNA phages and human RNA viruses through UTR trimming.

Bacterial ubiquitin ligase engineered for small molecule and protein target identification

This paper introduces SidBait, a modular proximity ligation approach to identify small molecule and protein targets, validated with known interactions and applied to discover new targets like CaMKII and CapZ.

Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization

The paper uses multimutant Salmonella strains to identify SPI-2 effector functions in gut inflammation and systemic colonization, finding SopD2 and GtgE critical for mucosal pathology.

Single-cell tracking of genetically minimized Salmonella reveals mechanisms of effector gene cooperation.

This study uses genome minimization and single-cell mass cytometry to identify effector genes in Salmonella that drive pathogen dissemination between immune cells.

Bacterial Effector Screening Reveals RNF214 as a Virus Restriction Factor in Mammals

This study identifies RNF214 as a mammalian antiviral factor using bacterial effectors, showing its role in restricting arbovirus infection through ubiquitination-mediated degradation.

Shigella flexneri evades LPS ubiquitylation through IpaH1.4-mediated degradation of RNF213

This study shows Shigella flexneri uses IpaH1.4 to degrade RNF213 and prevent LPS ubiquitylation, evading host antibacterial autophagy.

The Shigella flexneri effector IpaH1.4 facilitates RNF213 degradation and protects cytosolic bacteria against interferon-induced ubiquitylation

This study identifies IpaH1.4 as a Shigella effector that degrades RNF213, preventing ubiquitylation of intracellular bacteria and evading host immunity.