microscopy & imaging analysis

Therapeutic anti-IgE antibodies generate distinct functional immune complex signatures

This study shows that therapeutic anti-IgE antibodies form immune complexes with distinct functional properties, influenced by allergen incorporation and receptor binding patterns.

Stathmin-2 regulates microtubule dynamics and axonal resilience in human motor neurons

This study shows STMN2 regulates microtubule dynamics and axonal resilience in human motor neurons, linking cytoskeletal homeostasis to ALS pathology.

Multimodal imaging links molecular identity with nanoscale-resolved 3D cell morphology in opossum cortical cultures

This paper uses multimodal imaging to link molecular identity with nanoscale 3D cell morphology in opossum cortical cultures, showing how molecular changes correlate with morphological differences during development.

Cryo-EM structures of an Acinetobacter baumannii podovirus reveal a canonical capsid maturation

The paper reports the cryo-EM structures of an Acinetobacter baumannii podovirus, revealing its capsid maturation mechanism and providing a reference for phage engineering.

Quantitative live imaging reveals region-dependent medulloblastoma disseminationin the zebrafish brain

This study uses live imaging in zebrafish to show that hindbrain medulloblastoma cells spread more frequently than forebrain or midbrain tumors and that dasatinib reduces dissemination.

Persistent and recyclable myosin II minifilaments drive cortical contractility in the early C. elegans embryo

This study uses advanced microscopy to show that myosin II minifilaments persist and recycle in C. elegans embryos, contributing to cortical contractility through stable, static interactions with actin.

Breaking the bottleneck: semi-automated quantification of actin filament dynamics

The paper introduces SMAUG, a semi-automated tool for analyzing actin filament dynamics from TIRF microscopy images, reducing analysis time while maintaining accuracy.

Measuring neuronal input-output operations in vivo with random access projection microscopy

This paper introduces SLAP2, a high-speed microscope for measuring submicron synapses and dendritic voltage in behaving mice, revealing nonlinear input-output amplification in neurons.

siRNA/mRNA payload of lipid nanoparticles quantified using fluorescence microscopy and correlation spectroscopy

The paper quantifies mRNA and siRNA payload in lipid nanoparticles using fluorescence microscopy and FCS, showing size-dependent payload and coencapsulation dynamics.

Early Increases in Hippocampal Synchrony Predict Chronic Epileptiform Burden in a Temporal Lobe Epilepsy Model

Fluorescence microscopic evidence for intracellular silicon storage pools in diatoms and their partial separation

The paper uses fluorescence microscopy and subcellular fractionation to quantify intracellular silicon storage pools in diatoms under silicon starvation, finding concentrations up to 540 mM but lower in fractionated cytoplasm.

A Binuclear Cluster Supports Functional Asymmetry in Dark-Operative Protochlorophyllide Reductase

This paper identifies a binuclear [Cu2S2] cluster in DPOR that mediates functional asymmetry between its active sites.

Increased activity without changes in coupling between somata and apical dendrites during visual mismatches

This study examines neural responses in mouse visual cortex to natural and artificial visual stimuli, finding increased activity in response to natural mismatches but no change in dendritic-somatic coupling.

Prokaryotic homologs of the major vault protein form engineerable vault nanocapsules

The paper identifies prokaryotic homologs of the major vault protein that assemble into vault nanocapsules, revealing structural and size variability.

bL9 prevents recoding in collided ribosomes

This study shows that bL9 prevents recoding events in collided ribosomes by stabilizing their interaction and reducing frameshifting and hopping.

The Molecular Interplay of Tau and Motor Proteins on Microtubules

This study investigates how tau protein regulates motor proteins on microtubules using single-molecule imaging, cryo-EM, and MD simulations, revealing distinct mechanisms for inhibiting kinesin-1 and dynein.

Rapid Electro-optic Two-photon Bessel Light Sheet Fluorescence Lifetime Imaging for Tissue Volume Interrogation

This paper introduces a new two-photon fluorescence lifetime imaging technique for rapid volumetric imaging of biological samples with high resolution and low phototoxicity.

The Application of 3D Cryo-Volume Electron Microscopy to Visualize and Quantify the Photosynthetic Filamentous Cyanobacterium Anabaena

This paper uses cryo-vEM to visualize and quantify ultrastructural features of two Anabaena species without chemical pre-treatment.

Organ scale Three-dimensional atlas of developing pelvic innervation for the mouse lower urinary tract.

The paper creates a 3D atlas of pelvic innervation development in mice, revealing migration patterns, neurogenesis timing, and structural changes in pelvic ganglia across developmental stages.

FoxO1 and HNF1α cooperatively remodel nucleosome architecture and mechanical stability to activate transcription

This study shows FoxO1 and HNF1α work together to change nucleosome structure and stability to activate transcription.

Pain Matters: Nociceptive signaling drives neural encoding of inflammatory responses

The paper shows that nociceptive signaling is essential for the brain to represent and replay inflammatory states using human fMRI and mouse activity mapping.

Submembrane geography dictates GPCR signalling compartmentation and physiological function

This study shows that GPCR signaling varies based on submembrane location, with different functional outcomes in cardiomyocytes, and introduces submembrane-specific beta-blockers that target specific nanodomains.

Proteolytic feedback of quorum sensing promotes infection by Pseudomonas aeruginosa

This study shows that proteolytic feedback regulates quorum sensing in Pseudomonas aeruginosa by controlling RhlR stability, affecting virulence and biofilm formation.

Mapping AMPA and NMDA receptors at mouse brain hippocampal synapses by cryo-ET

The paper maps AMPA and NMDA receptors at mouse hippocampal synapses using cryo-ET, revealing their distribution and relationship to glutamate release sites.

Comparative cryo-EM structures of CGMMV and ToMMV reveal divergent coat protein packing and electrostatics indicating Tobamovirus host specificity

Peripheral Axons Form Endoplasmic Reticulum-Rich Conduits With Breast Cancer Cells

The paper identifies ER-rich conduits formed by peripheral axons and breast cancer cells, revealing a structural interface for potential intercellular communication.

Light-sheet fluorescence microscopy of optically cleared human head-and-neck organs: three-dimensional tissue assessment and histological correlation

This study uses optical clearing and light-sheet fluorescence microscopy to visualize 3D microanatomy of human head-and-neck tissues and correlate it with conventional histology.

Tissue geometry patterns epidermal stem cell fate in murine interfollicular skin

The paper shows that dermal-epidermal junction geometry influences stem cell fate in mouse skin, with concave areas promoting proliferation and convex areas promoting differentiation.

Brain-wide representations of olfactory behavior in C. elegans

The paper analyzes brain-wide neural activity in C. elegans during olfactory navigation and identifies functional circuits linking sensory input to motor behavior across different environments and behavioral states.

Open filament tracks drive Bacteroidota gliding motility

The paper uses 3D super-resolution tracking to show that Bacteroidota gliding involves multiple open filament tracks, challenging the single closed loop model.